低压低氧暴露条件下肺动脉高压大鼠循环microRNA表达及其意义

发布时间：2018-07-06 08:10

本文选题：低氧性肺动脉高压 + 循环miRNA　；参考：《西安交通大学学报(医学版)》2016年04期

【摘要】：目的研究低压低氧暴露条件下肺动脉高压(HPH)模型大鼠循环microRNA(miRNA)表达的变化。方法利用基因芯片技术定量检测、分析HPH组及对照组大鼠血清中潜在的循环miRNA的表达及其差异。基于病例-对照设计,采用荧光定量PCR技术对差异化表达miRNA进行验证。受试者工作特征曲线(ROC)分析测试4种差异化表达miRNA对HPH组及对照组的鉴别效能。结果与对照组比较,HPH组大鼠13种miRNA表达上调,10种miRNA表达下调,其中,荧光定量PCR初步证实miR-451、miR-505及let-7d表达上调,而miR-214表达下调。ROC分析结果显示,miR-451、miR-505及let-7d用于鉴别HPH组大鼠和对照组大鼠的曲线下面积(AUC)分别为0.979、0.938和0.993。结论低压低氧暴露条件下HPH组大鼠与对照组大鼠循环miRNA的表达存在显著差异,提示血清miRNA差异化表达可能与HPH病理变化相关。
[Abstract]:Objective to study the changes of microRNA (miRNA) expression in rats with pulmonary hypertension (HPH) exposed to hypobaric hypoxia. Methods the expression and difference of potential circulating miRNA in serum of HPH group and control group were analyzed quantitatively by gene chip technique. Based on the case-control design, the differential expression of miRNA was verified by fluorescence quantitative PCR. The differential expression of miRNA in HPH group and control group was evaluated by operating characteristic curve (ROC). Results compared with the control group, the expression of 13 miRNAs was up-regulated and 10 miRNAs were down-regulated in HPH group. The expression of miR-451miR-505 and let-7d was preliminarily confirmed by fluorescence quantitative polymerase chain reaction (FQ-PCR). The results of down-regulation of miR-214 expression showed that the area under curve (AUC) of miR-451mmiR-505 and let-7d in differentiating HPH group and control group were 0.999 and 0.993, respectively. Conclusion there is a significant difference in the expression of circulating miRNA between HPH group and control group under hypobaric hypoxia, suggesting that the differential expression of miRNA in serum may be related to the pathological changes of HPH.
【作者单位】：开封市中心医院检验科;兰州军区乌鲁木齐总医院;
【基金】：新疆维吾尔自治区自然科学基金资助项目(No.2015211C233)~~
【分类号】：R544.1;R440

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