实时荧光定量PCR对结核病潜伏感染快速诊断的研究

发布时间：2018-07-07 17:30

  本文选题：结核病 + 潜伏感染　； 参考：《中国病原生物学杂志》2016年04期

【摘要】：目的探讨7种细胞因子的mRNA表达水平作为诊断结核分枝杆菌潜伏感染生物标志物的可能性。方法健康对照64人,活动性肺结核50人,结核分枝杆菌潜伏感染60人,采用实时荧光定量PCR(qRT-PCR)检测结核特异性抗原刺激的外周血中TNF-α、IFN-γ、IL-2、IL-10、IFI35、CXCL10及Foxp3的mRNA表达水平。结果 7种因子相对表达量在活动性肺结核组分别为2.33±0.09、4.94±0.45、150±17.82、2.02±0.55、9.53±5.96、5.56±0.97、1.24±0.17,潜伏感染组分别为3.2±0.61、10.04±1.89、202±17.6、21.89±10.25、29.17±11.19、41.35±11.46、2.14±0.67,健康组分别为1.2±0.08、2.92±0.03、53±17.82、1.48±0.17、1.28±0.77、1.63±0.25、1.03±0.67。潜伏感染组与健康组相比7种细胞因子的mRNA表达水平,差异均有统计学意义(P0.01),相应细胞因子检测的敏感性、特异性分别为95.1%、82.5%,92.5%、88.7%,91.9%、90.1%,88.2%、51.5%,74.2%、61.2%,81.8%、73.4%和80.4%、51.3%;潜伏感染组和活动性结核组比较除IFI35因子外,其余6种细胞因子的mRNA表达水平差异均有统计学意义(P0.05),相应细胞因子检测的特异性、敏感性分别为90.6%、80%,88.6%、92.7%,91.9%、86.2%,57.6%、60%,50%、58%,76%、72.2%和66.7%、67.1%;健康对照组和活动性结核组相比较,7种细胞因子的mRNA表达水平差异均有统计学意义(P0.01),相应细胞因子检测的敏感性、特异性分别为92.3%、90%,88.6%、86.7%,94.4%、86.7%,89.7%、65.8%,82.4%、63.3%,88.2%、70%和92.9%、72.1%。结核病密切接触者潜伏感染率为48.97%,健康组潜伏感染率为36%。结论结核病患者血清TNF-α、IFN-γ、IL-2mRNA水平升高,以潜伏感染者升高更显著,因此可作为诊断结核分枝杆菌潜伏感染的生物标志物。
[Abstract]:Objective to explore the possibility of mRNA expression of seven cytokines as biomarkers for the diagnosis of latent infection of Mycobacterium tuberculosis. Methods A total of 64 healthy controls, 50 active pulmonary tuberculosis patients and 60 patients with latent infection of Mycobacterium tuberculosis were used to detect the mRNA expression of TNF- 伪, IFN- 纬, IL-2IL-10, IL-10, IFI35, CXCL10 and Foxp3 mRNA in peripheral blood stimulated by specific antigen of tuberculosis (QRT-PCR). Results the relative expression levels of seven factors in active pulmonary tuberculosis group were 2.33 卤0.09 卤4.94 卤0.45150 卤17.82 卤2.02 卤0.559.53 卤5.965.56 卤0.971.24 卤0.17, respectively, and those in latent infection group were 3.2 卤0.61n 10.04 卤1.89202 卤17.629.17 卤10.2529.17 卤11.1929.17 卤11.466.2.14 卤0.67and 1.2 卤0.082.92 卤0.0353 卤17.821.48 卤0.171.28 卤0.771.63 卤0.251.03 respectively. The mRNA expression levels of 7 cytokines in latent infection group were significantly higher than those in healthy group (P0.01). The sensitivity and specificity of the corresponding cytokine detection were 95.1, 82.5 and 92.5, respectively. The sensitivity of the corresponding cytokines in the latent infection group was 73.4% and 80.4% and 51.3%, respectively. The sensitivity of the corresponding cytokines was 95.1% and 82.5%, respectively. The sensitivity of the corresponding cytokines in the latent infection group was higher than that in the active group, and the specificity was 91.9%. The sensitivity of the corresponding cytokines in the latent infection group was 73.4% and 80.4% in the active group, except IFI35 in the latent infection group and the active group, in addition to IFI35, the difference between the latent infection group and the active group was significant. The mRNA expression levels of the other six cytokines were significantly different (P0.05). 鏁忔劅鎬у垎鍒�涓�90.6%,80%,88.6%,92.7%,91.9%,86.2%,57.6%,60%,50%,58%,76%,72.2%鍜，

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