河南省德尔卑沙门菌和阿贡纳沙门菌耐药与分子分型研究

发布时间：2018-07-10 03:30

  本文选题：德尔卑沙门菌 + 阿贡纳沙门菌　； 参考：《中国病原生物学杂志》2016年06期

【摘要】：目的分析2009-2012年河南省德尔卑沙门菌和阿贡纳沙门菌临床分离株的耐药状况与脉冲场凝胶电泳(PFGE)带型,为以德尔卑沙门菌、阿贡纳沙门菌等非伤寒沙门菌为代表的食源性疾病暴发预警、调查、溯源及公共卫生意义上的抗生素使用策略提供基线与参考数据。方法根据国际PulseNet细菌性传染病分子分型监测网络公布的非伤寒沙门菌PFGE分型技术与美国临床与实验室标准协会(CLSI)沙门菌K-B法药敏测试方案,对2009-2012年分离自河南省5个哨点医院的德尔卑沙门菌与阿贡纳沙门菌进行13种抗生素的药敏测试及PFGE脉冲场凝胶电泳分子分型分析。结果 76株德尔卑沙门菌与84株阿贡纳沙门菌对8类13种抗生素均有不同程度的耐药,耐3种以上抗生素的有141株(88.13%)。其中耐3~5种抗生素38株(占23.75%),耐6~8种抗生素66株(占41.25%),耐9~10种抗生素22株(占13.75%),耐11~12种抗生素15株(占9.38%)。84株阿贡纳沙门菌经XbaⅠ酶切与脉冲场凝胶电泳后获得36种带型,每种带型包含1~9个菌株,相似度50.77%~100%,以AGN27与AGN33为主要优势带型。76株德尔卑沙门菌经XbaⅠ酶切与脉冲场凝胶电泳后获得41种带型,每种带型包含菌株数1~12个菌株,相似度43.25%~100%,以DER17与DER34为主要优势带型。结论河南省临床分离株德尔卑沙门菌和阿贡纳沙门菌耐药状况较严重,PFGE带型呈现出多样性的同时又具有较显著的优势带型特点,部分带型与其对应的耐药谱具有一定的关联性及相同的聚集性。
[Abstract]:Objective to analyze the drug resistance and pulsed field gel electrophoresis (PFGE) pattern of clinical isolates of Salmonella del Pei and Salmonella Argonella from 2009 to 2012 in Henan Province. Non-typhoid Salmonella, such as Salmonella Argonella, is the representative of food-borne disease outbreak early warning, investigation, traceability and public health sense of antibiotic use strategies to provide baseline and reference data. Methods according to the PFGE typing technique of non-typhoid Salmonella and the K-B drug sensitivity test program of the American Association of Clinical and Laboratory Standards (CLSI) published by the International PulseNet Monitoring Network for Bacteriological Infectious Diseases. Thirteen antibiotics of Salmonella delpezii and Salmonella Argonella isolated from 5 sentinel hospitals in Henan Province from 2009 to 2012 were tested for their drug sensitivity and molecular typing by PFGE pulsed field gel electrophoresis (PFGE). Results 76 strains of Salmonella delpezii and 84 strains of Argonella were resistant to 8 kinds of 13 kinds of antibiotics, 141 strains (88.13%) were resistant to more than 3 kinds of antibiotics. Among them, 38 strains (23.75%) were resistant to 5 kinds of antibiotics, 66 strains (41.25%) were resistant to 68 kinds of antibiotics, 22 strains (13.75%) were resistant to 9 or 10 kinds of antibiotics, 15 strains (9.38%) were resistant to 1112 kinds of antibiotics. Each band type consisted of 1 ~ 9 strains, and the similarity was 50.77100. With AGN27 and AGN33 as the main dominant bands, 41 bands were obtained by XBA 鈪，

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