甲状腺术后切口耐甲氧西林金黄色葡萄球菌耐药基因研究
发布时间：2018-08-09 15:35
【摘要】：目的 了解甲状腺术后切口感染耐甲氧西林金黄色葡萄球菌(MRSA)耐药及耐药基因检测情况。方法 收集2011-2014年长春地区医院甲状腺术后切口感染标本,采用西门子公司MicroScan WalkAway-40全自动微生物鉴定系统鉴定细菌,采用K-B法对MRSA进行药敏试验;提取细菌DNA,设计特异性基因进行PCR扩增。结果 2011-2014年共分离21株MRSA,对15种临床常用抗生素耐药率分别为:青霉素100%,阿莫西林95.24%,苯唑西林95.24%,氨卡西林100%,四环素71.43%,克林霉素80.95%,庆大霉素85.71%,头孢唑林100%,左氧氟沙星71.43%,莫西沙星38.1%,磺胺甲VA唑/甲氧苄啶42.86%,喹奴普汀/达福普汀4.76%,利福平38.10%,利奈唑胺4.76%,未检出万古霉素耐药菌株。21株MRSA中mecA、femA、16SrRNA和orfX基因检出率均为100%,hla阳性率为85.71%(18/21),icaA和clfB阳性率均为80.95%(17/21),aac(6′)/aph(2′′)阳性率为57.14%(12/21),hlg-2和pvl阳性率均为76.19%(16/21),clfA阳性率为71.43%(15/21),lukE阳性率为66.67%(14/21),ermC阳性率为52.38%(11/21),aac(3′)-Ⅲ阳性率为47.62%(10/21),ermA阳性率为28.57%(6/21),SasX和hlb阳性率均为23.81%(5/21),未检测到ermB基因。讨论MRSA是医院院内感染常见致病菌,喹奴普汀/达福普汀、利奈唑胺和万古霉素可用于长春地区MRSA感染的治疗。
[Abstract]:Objective to investigate the detection of methicillin-resistant Staphylococcus aureus (MRSA) resistance and drug resistance genes in incision infection after thyroid surgery. Methods the specimens of incision infection after thyroid surgery in Changchun region from 2011 to 2014 were collected. The bacteria were identified by MicroScan WalkAway-40 automatic microbiological identification system of Siemens Company. The drug sensitivity test of MRSA was carried out by K-B method. Bacterial DNA was extracted and specific genes were designed for PCR amplification. Results A total of 21 MRSAs were isolated from 2011 to 2014. The resistance rates to 15 commonly used antibiotics were as follows: penicillin 100, amoxicillin 95.2424, oxacillin 95.24m, amoxicillin 100, tetracycline 71.43, clindamycin 80.955, gentamicin 85.71, cefazolin 100, levofloxacin 100. 71.43, moxifloxacin 38.1, sulfamethoxazole / trimethoprim 42.86, quinoplatin / dafopentin 4.76, rifampicin 38.1010, linazolamine 4.76. the positive rates of mecAfemAfemAmefen 16s rRNA and orfX gene in MRSA were 85.71% (18 / 21) and 85.71% (18 / 21), respectively. The positive rates of MecAFEMA 16s rRNA and orfX gene were 85.71% (18 / 21), 85.71% (18 / 21), and 85.71% (18 / 21), respectively. 闃虫，
本文编号：2174541
[Abstract]:Objective to investigate the detection of methicillin-resistant Staphylococcus aureus (MRSA) resistance and drug resistance genes in incision infection after thyroid surgery. Methods the specimens of incision infection after thyroid surgery in Changchun region from 2011 to 2014 were collected. The bacteria were identified by MicroScan WalkAway-40 automatic microbiological identification system of Siemens Company. The drug sensitivity test of MRSA was carried out by K-B method. Bacterial DNA was extracted and specific genes were designed for PCR amplification. Results A total of 21 MRSAs were isolated from 2011 to 2014. The resistance rates to 15 commonly used antibiotics were as follows: penicillin 100, amoxicillin 95.2424, oxacillin 95.24m, amoxicillin 100, tetracycline 71.43, clindamycin 80.955, gentamicin 85.71, cefazolin 100, levofloxacin 100. 71.43, moxifloxacin 38.1, sulfamethoxazole / trimethoprim 42.86, quinoplatin / dafopentin 4.76, rifampicin 38.1010, linazolamine 4.76. the positive rates of mecAfemAfemAmefen 16s rRNA and orfX gene in MRSA were 85.71% (18 / 21) and 85.71% (18 / 21), respectively. The positive rates of MecAFEMA 16s rRNA and orfX gene were 85.71% (18 / 21), 85.71% (18 / 21), and 85.71% (18 / 21), respectively. 闃虫，
本文编号：2174541
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