3种检测肺炎支原体感染方法的比较分析
发布时间：2018-08-26 12:29
【摘要】：目的:比较3种检测方法对肺炎支原体(MP)感染的临床诊断效果及应用价值。方法:对疑为呼吸系统MP感染的494例住院患儿留取痰液,用PCR荧光探针法进行MP DNA检测,用被动凝集法和间接免疫荧光法进行血清抗MP抗体检测。结果:PCR荧光探针法阳性299例(60.53%),被动凝集法阳性290例(58.70%),间接免疫荧光法阳性242例(48.99%),PCR荧光探针法与被动凝集法的阳性率无差别(χ2=0.341,P0.012 5),间接免疫荧光法与PCR荧光探针法(χ2=13.274,P0.012 5)、被动凝集法(χ2=9.383,P0.012 5)阳性率相比,差异均有统计学意义。被动凝集法阳性结果中滴度为40的患儿共77例,其中70例的另2种方法结果均为阳性。结论:间接免疫荧光法的检测阳性率低于PCR荧光探针法与被动凝集法。对于儿童,被动凝集法静脉血标本的采集较PCR荧光探针法的痰标本采集更为简便,可将被动凝集法作为MP感染的门诊筛查项目;被动凝集法应将滴度≥40的结果判定为阳性。
[Abstract]:Objective: to compare the clinical diagnostic effect and application value of three detection methods for mycoplasma pneumoniae (MP) infection. Methods: sputum was collected from 494 hospitalized children with suspected respiratory MP infection, MP DNA was detected by PCR fluorescence probe method, serum anti-MP antibody was detected by passive agglutination method and indirect immunofluorescence method. Results there were 299 cases (60.53%) positive by PCR fluorescence probe method, 290 cases (58.70%) positive by passive agglutination method, and 242cases (48.99%) positive by indirect immunofluorescence method. There was no difference between PCR fluorescence probe method and passive agglutination method (蠂 20.341U P 0.0125), indirect immunofluorescence assay and PCR fluorescence probe method (蠂 213.274U P 0.012). 5) the positive rate of the passive agglutination method (蠂 29.383 P 0.012 5) was higher than that of the control group. The difference was statistically significant. There were 77 children with 40 titers in the positive results of the passive agglutination method, of which the other two methods were all positive. Conclusion: the positive rate of indirect immunofluorescence assay is lower than that of PCR fluorescence probe method and passive agglutination method. For children, the collection of venous blood samples by passive agglutination method is more convenient than that of sputum samples collected by PCR fluorescence probe method. Passive agglutination method can be used as an outpatient screening item for MP infection, and the results of titer 鈮，
本文编号：2204874
[Abstract]:Objective: to compare the clinical diagnostic effect and application value of three detection methods for mycoplasma pneumoniae (MP) infection. Methods: sputum was collected from 494 hospitalized children with suspected respiratory MP infection, MP DNA was detected by PCR fluorescence probe method, serum anti-MP antibody was detected by passive agglutination method and indirect immunofluorescence method. Results there were 299 cases (60.53%) positive by PCR fluorescence probe method, 290 cases (58.70%) positive by passive agglutination method, and 242cases (48.99%) positive by indirect immunofluorescence method. There was no difference between PCR fluorescence probe method and passive agglutination method (蠂 20.341U P 0.0125), indirect immunofluorescence assay and PCR fluorescence probe method (蠂 213.274U P 0.012). 5) the positive rate of the passive agglutination method (蠂 29.383 P 0.012 5) was higher than that of the control group. The difference was statistically significant. There were 77 children with 40 titers in the positive results of the passive agglutination method, of which the other two methods were all positive. Conclusion: the positive rate of indirect immunofluorescence assay is lower than that of PCR fluorescence probe method and passive agglutination method. For children, the collection of venous blood samples by passive agglutination method is more convenient than that of sputum samples collected by PCR fluorescence probe method. Passive agglutination method can be used as an outpatient screening item for MP infection, and the results of titer 鈮，
本文编号：2204874
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