新型蛋白质芯片与荧光酶联免疫法检测过敏原特异性IgE的对比研究

发布时间：2018-09-02 05:32

【摘要】：目的:为确认新型过敏原特异性Ig E抗体检测试剂盒(芯片法)的可信度,将芯片法与荧光酶联免疫法进行对比分析。方法:芯片法运用荧光免疫吸附分析的原理,针对6种过敏原的特异性Ig E进行检测,并对比与荧光酶联免疫法检测结果的阳性一致率、阴性一致率、整体一致率,探讨kappa值。共有456例样本用于对比分析。结果:芯片法所检测的阳性率分别为屋尘螨88.6%、猫毛皮屑92.9%、鸡蛋白92.5%、虾91.5%、蚌99.3%、蟹92.2%;而荧光酶联免疫法的阳性率分别为屋尘螨88.6%、猫毛皮屑92.9%、鸡蛋白92.5%、虾85.2%、蚌93.1%、蟹92.2%。6种过敏原的kappa值分别为屋尘螨1.000、猫皮屑1.000、鸡蛋白1.000、虾0.695、蚌0.172与蟹1.000。对于4种常见过敏原屋尘螨、猫皮屑、鸡蛋白与蟹,芯片法与荧光酶联免疫法的测定结果完全一致。虾与蚌的检测一致性偏低,可能因为阴性样本数偏低、检测值位于阈值附近及芯片法敏感度较高所导致。结论:芯片法与荧光酶联免疫法检测变应原特异性Ig E具有高度一致性,芯片法的检测结果敏感性高,是临床上过敏原特异性Ig E检测的可靠工具。
[Abstract]:Aim: to confirm the reliability of the new allergen specific Ig E antibody detection kit (chip method), the chip assay and the fluorescence enzyme linked immunosorbent assay (FIA) were compared and analyzed. Methods: the principle of fluorescence immunosorbent assay (FIA) was used to detect the specific Ig E of 6 allergens. The positive, negative and overall kappa values were compared with the results of fluorescence enzyme-linked immunosorbent assay (FIA). A total of 456 samples were used for comparative analysis. Results: the positive rates of the microarray method were 88.6m, 92.9cm, 92.5, 91.5, 99.3and 92.2respectively, while the positive rates of fluorescence enzyme-linked immunosorbent assay were 88.6m for house dust mite, 92.9for cat hair dander, 92.5 for egg white, 85.2for shrimp, 93.1for mussel, 92.2for crab. The kappa values of allergen were 1.000 for house dust mite, 1.000 for cat dander, 1.000 for egg white, 0.695 for shrimp, 0.172 for mussel and 1.000for crab. For four common allergen house dust mites, cat dandruff, egg white and crab, the results of microarray and fluorescence enzyme linked immunosorbent assay were in good agreement. The low consistency between shrimp and clam may be due to the low number of negative samples, the detection value located near the threshold value and the high sensitivity of microarray method. Conclusion: the detection of allergen-specific Ig E by microarray and fluorescence enzyme-linked immunosorbent assay is highly consistent, and the sensitivity of microarray assay is high. It is a reliable tool for the detection of allergen specific Ig E in clinic.
【作者单位】： 北京大学第三医院皮肤科;
【分类号】：R446.6
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本文编号：2218396