耐碳青霉烯类鲍氏不动杆菌基因群检测研究
发布时间：2018-10-22 07:23
【摘要】：目的探讨耐碳青霉烯类鲍氏不动杆菌(CRAB)基因群和合理用药的相关性,为临床用药提供参考依据。方法选取2014年1月-2015年7月无锡市第二人民医院,耐碳青霉烯类鲍氏不动杆菌感染阳性患者217例,进行PCR基因序列的分析;记录用药情况和预后,对基因群和联合用药做相关性分析。结果 67株扩增出OXA-23型引物预期长度产物;169株扩增出OXA-51(66)型引物预期长度产物;预后与是否联合用药做交叉表显示,预后和联合用药差异有统计学意义(P0.05)。结论耐碳青霉烯类鲍氏不动杆菌基因群OXA-51(66)的阳性与阴性表达,检测其基因序列对于临床联合用药具有一定的提示价值。
[Abstract]:Objective to investigate the correlation between (CRAB) gene group of acinetobacter baumannii and rational drug use, and to provide reference for clinical use. Methods 217 patients with acinetobacter baumannii infection were selected from Wuxi second people's Hospital from January 2014 to July 2015. The sequence of PCR gene was analyzed and the drug use and prognosis were recorded. The correlation between gene group and combined drug use was analyzed. Results the expected length product of OXA-23 primer was amplified from 67 strains and the expected length product of OXA-51 (66) type primer from 169 strains was amplified. Conclusion the positive and negative expression of OXA-51 _ (66) in acinetobacter baumannii gene group is positive and negative, and the detection of its gene sequence may be helpful for clinical use of acinetobacter baumannii.
【作者单位】： 江苏大学医学院;南京中医药大学无锡附属医院无锡市中医医院检验科;江苏大学医学院学院办;
【基金】：江苏省无锡市科技指导性资助项目(CSZ00N1212)
【分类号】：R440
，
本文编号：2286490
[Abstract]:Objective to investigate the correlation between (CRAB) gene group of acinetobacter baumannii and rational drug use, and to provide reference for clinical use. Methods 217 patients with acinetobacter baumannii infection were selected from Wuxi second people's Hospital from January 2014 to July 2015. The sequence of PCR gene was analyzed and the drug use and prognosis were recorded. The correlation between gene group and combined drug use was analyzed. Results the expected length product of OXA-23 primer was amplified from 67 strains and the expected length product of OXA-51 (66) type primer from 169 strains was amplified. Conclusion the positive and negative expression of OXA-51 _ (66) in acinetobacter baumannii gene group is positive and negative, and the detection of its gene sequence may be helpful for clinical use of acinetobacter baumannii.
【作者单位】： 江苏大学医学院;南京中医药大学无锡附属医院无锡市中医医院检验科;江苏大学医学院学院办;
【基金】：江苏省无锡市科技指导性资助项目(CSZ00N1212)
【分类号】：R440
，
本文编号：2286490
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