耐碳青霉烯类肺炎克雷伯菌的耐药性及同源性分析

发布时间：2018-11-14 12:19

【摘要】：目的:研究本院耐碳青霉烯类肺炎克雷伯菌(CRKP)耐药性以及菌株间的同源性。方法:全自动微生物分析仪进行细菌鉴定及药敏试验,采用改良Hodge试验和EDTA协同试验检测耐药表型,PCR检测碳青霉烯酶基因,脉冲场凝胶电泳(PFGE)分析菌株同源性。结果:26株肺炎克雷伯菌对头孢菌素类、单环β-内酰胺类、氨基糖苷类、喹喏酮类、呋喃妥因等多种抗菌药物耐药;经PCR检测后25株携带KPC基因,1株携带IMP基因,未检测出VIM、NDM-1、OXA-48基因;PFGE电泳结果显示,26株肺炎克雷伯菌分为4型。其中Ⅰ、Ⅱ为主要流行克隆型。结论:本院存在肺炎克雷伯菌耐药株的传播,携带的耐药基因以KPC-2为主,主要以ICU与MICU的2株耐药株流行,而且耐药菌株间存在着不同程度的亲缘关系。
[Abstract]:Objective: to study the (CRKP) resistance of Klebsiella pneumoniae resistant to carbapenem and its homology. Methods: the bacterial identification and drug sensitivity test were carried out by automatic microbiological analyzer. The phenotype of drug resistance was detected by modified Hodge test and EDTA co-assay. The carbapenase gene was detected by PCR and the homology of the strains was analyzed by pulsed field gel electrophoresis (PFGE). Results: 26 strains of Klebsiella pneumoniae were resistant to cephalosporins, monocyclic 尾 -lactams, aminoglycosides, quinolones and furantoin. After PCR detection, 25 strains carried KPC gene, 1 strain carried IMP gene, and no VIM,NDM-1,OXA-48 gene was detected. The results of PFGE electrophoresis showed that 26 strains of Klebsiella pneumoniae were classified into 4 types. Among them, 鈪，

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