白城地区动物源链球菌耐药性检测及优化临床用药方案的研究
发布时间：2018-11-24 12:51
【摘要】：链球菌是引起人畜共患病的主要病原之一,常可引起猪脑膜炎、败血症,犊牛菌血症、心内膜炎,奶牛乳房炎,羊急性或慢性呼吸道传染病等疾病。链球菌引发的疾病主要依赖抗生素的治疗,但近年来链球菌逐渐出现了耐药株,因耐药造成的抗感染失败屡见不鲜。为此,本研究对吉林省白城地区养殖场内患有仔猪脑膜炎、败血症,奶牛乳房炎的病畜进行了病原菌的分离鉴定,并对其分离获得的链球菌进行耐药性检测、耐药基因检测并筛选能减少链球菌耐药突变选择窗的中西药合剂,以期优化临床用药方案,减少因耐药菌产生造成的经济损失。本试验从我省白城地区的患病猪场、牛场采集病料121头份,通过选择鉴别培养基、革兰氏染色、分子生物学方法对分离菌株进行鉴定,获得链球菌33株。采用琼脂二倍稀释法对分离菌株进行耐药性检测,结果显示,分离菌株均为多重耐药株,对6类常见抗菌药物(β-内酰胺类、氟喹诺酮类、四环素类、氯霉素类、磺胺类、大环内酯类)呈不同程度的交叉耐药,12%的分离菌对以上6类抗菌药物均耐药,61%以上对其中4类或5类药物耐药,而对强力霉素和氟苯尼考的交叉耐药率最低,为24.2%。对33株动物源链球菌进行了29个主要耐药基因的检测,其中P8、P9株携带耐药基因最多;P20、C2株携带耐药基因最少;57.1%的菌株携带7个以上的耐药基因。.采用平皿二倍稀释法测分离菌防耐药突变浓度(MPC),确定分离菌株对抗菌药物的耐药突变选择窗(MSW),并测定链球菌对瓜蒌、佩兰醇提物的最小抑菌浓度(MIC),采用棋盘法测定其联合抑菌指数(FIC),结果表明瓜蒌醇提物与环丙沙星,瓜蒌醇提物与氟苯尼考联合抑制链球菌呈现相加作用;佩兰醇提物与环丙沙星联合抑制链球菌呈现协同作用;佩兰醇提物与强力霉素联合抑制链球菌呈现相加作用,采用微量稀释法进而确定MSW。其结果显示,中西药联合可以缩小链球菌对单药的MSW,能够有效抑制耐药菌株的出现。
[Abstract]:Streptococcus is one of the main pathogens of zoonosis, which can cause swine meningitis, septicemia, calves bacteremia, endocarditis, cow mastitis, sheep acute or chronic respiratory infectious diseases and so on. The disease caused by streptococcus mainly depends on the treatment of antibiotics, but in recent years, streptococcus has gradually appeared drug-resistant strains, because of drug resistance caused by the failure of anti-infection is common. Therefore, in this study, the pathogenic bacteria were isolated and identified from piglet meningitis, septicemia and cow mastitis in the breeding farm of Baicheng, Jilin Province, and the drug resistance of streptococcus isolated from them was detected. In order to optimize the clinical drug use plan and reduce the economic loss caused by drug-resistant bacteria, the drug resistance gene detection and screening can reduce the mutation window of streptococcus drug resistance in Chinese and western medicine mixture. In this experiment, 121 samples of diseased materials were collected from the diseased pig farm in Baicheng area of our province. 33 strains of streptococcus were obtained by selecting identification medium, Gram staining and molecular biology method. Agar dilution method was used to detect the drug resistance of the isolated strains. The results showed that the isolates were multidrug resistant, and were resistant to 6 common antibiotics (尾 -lactam, fluoroquinolones, tetracyclines, chloramphenicol, sulfonamides). Among them, 12% isolates were all resistant to the above 6 kinds of antimicrobial agents, and more than 61% were resistant to 4 or 5 of them, while the cross resistance rates to doxycycline and florfenicol were the lowest. For 24.2. Thirty three strains of Streptococcus from animals were tested for drug resistance genes. Among them, P8 P9 strain carried the most drug resistance genes, P20C 2 strain carried the least drug resistance genes, and 57.1% strains carried more than 7 drug resistance genes. Double dilution method was used to determine the antimicrobial resistance mutagenesis window (MSW),) and the minimal inhibitory concentration (MIC),) of Streptococcus to Trichosanthes and Peracetamol extracts (MIC),). The combined bacteriostatic index (FIC),) was determined by chessboard method. The results showed that the alcohol extract of Trichosanthes and ciprofloxacin, the alcohol extract of Trichosanthes and florfenicol combined with florfenicol could inhibit streptococcus. The synergistic effect of perlain alcohol extract and ciprofloxacin on the inhibition of streptococcus was observed, and the synergistic effect of perlain alcohol extract and doxycycline on the inhibition of streptococcus was found. The microdilution method was used to determine MSW.. The results showed that the combination of Chinese and western medicine could effectively inhibit the emergence of drug-resistant strains by reducing the MSW, of streptococcus to a single drug.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.5
本文编号：2353783
[Abstract]:Streptococcus is one of the main pathogens of zoonosis, which can cause swine meningitis, septicemia, calves bacteremia, endocarditis, cow mastitis, sheep acute or chronic respiratory infectious diseases and so on. The disease caused by streptococcus mainly depends on the treatment of antibiotics, but in recent years, streptococcus has gradually appeared drug-resistant strains, because of drug resistance caused by the failure of anti-infection is common. Therefore, in this study, the pathogenic bacteria were isolated and identified from piglet meningitis, septicemia and cow mastitis in the breeding farm of Baicheng, Jilin Province, and the drug resistance of streptococcus isolated from them was detected. In order to optimize the clinical drug use plan and reduce the economic loss caused by drug-resistant bacteria, the drug resistance gene detection and screening can reduce the mutation window of streptococcus drug resistance in Chinese and western medicine mixture. In this experiment, 121 samples of diseased materials were collected from the diseased pig farm in Baicheng area of our province. 33 strains of streptococcus were obtained by selecting identification medium, Gram staining and molecular biology method. Agar dilution method was used to detect the drug resistance of the isolated strains. The results showed that the isolates were multidrug resistant, and were resistant to 6 common antibiotics (尾 -lactam, fluoroquinolones, tetracyclines, chloramphenicol, sulfonamides). Among them, 12% isolates were all resistant to the above 6 kinds of antimicrobial agents, and more than 61% were resistant to 4 or 5 of them, while the cross resistance rates to doxycycline and florfenicol were the lowest. For 24.2. Thirty three strains of Streptococcus from animals were tested for drug resistance genes. Among them, P8 P9 strain carried the most drug resistance genes, P20C 2 strain carried the least drug resistance genes, and 57.1% strains carried more than 7 drug resistance genes. Double dilution method was used to determine the antimicrobial resistance mutagenesis window (MSW),) and the minimal inhibitory concentration (MIC),) of Streptococcus to Trichosanthes and Peracetamol extracts (MIC),). The combined bacteriostatic index (FIC),) was determined by chessboard method. The results showed that the alcohol extract of Trichosanthes and ciprofloxacin, the alcohol extract of Trichosanthes and florfenicol combined with florfenicol could inhibit streptococcus. The synergistic effect of perlain alcohol extract and ciprofloxacin on the inhibition of streptococcus was observed, and the synergistic effect of perlain alcohol extract and doxycycline on the inhibition of streptococcus was found. The microdilution method was used to determine MSW.. The results showed that the combination of Chinese and western medicine could effectively inhibit the emergence of drug-resistant strains by reducing the MSW, of streptococcus to a single drug.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.5
【参考文献】
相关期刊论文 前1条
1 芮萍;马增军;段玲欣;刘谢荣;倪静;沈建忠;;猪链球菌对氟喹诺酮类药物的耐药性与靶位突变相关性[J];畜牧兽医学报;2011年04期
，本文编号：2353783
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