褪色沙雷菌耐药率与多位点序列分析研究

发布时间：2018-11-28 12:49

【摘要】：目的研究褪色沙雷菌的耐药率与分子流行病学规律,建立多位点序列分析方法,明确其进化特征,为临床针对性使用抗菌药物和防控此致病菌感染提供依据。方法对2013年9月-2014年12月分离的40株褪色沙雷菌进行体外药物敏感性试验与多位点序列分析(MLSA),并用Splits tree与CLUSTALW软件进行生物信息学分析,揭示其流行趋势。结果体外药敏试验结果表明,40株褪色沙雷菌对13种抗菌药物的耐药率在10.0%~52.5%,对亚胺培南耐药率为32.5%;MLSA设计4个管家基因,GC含量约为60.0%;各等位基因数分布于14~16,各多态性位点数分布于33~74,gyrB的多态性位点数最多(74个);Splits tree软件分裂分解分析结果显示,大部分褪色沙雷菌聚在一起,形成一个克隆复合体;CLUSTALW软件聚类分析结果显示,40株褪色沙雷菌形成19个ST型,其中ST8型12株,且ST8型是耐碳青霉烯类抗菌药物褪色沙雷菌的流行与暴发型,ST8、ST9、ST10与ST11形成一个克隆复合体CC8。结论褪色沙雷菌对碳青霉烯类抗菌药物耐药率较高,且多为泛耐药菌株;褪色沙雷菌的分子流行病学趋势相对较慢,在基因组上高度保守,目前需要重点监测以ST8型为代表褪色沙雷菌CC8克隆复合体的流行,以防其在医院的大规模流行与暴发。
[Abstract]:Objective to study the drug resistance rate and molecular epidemiology of Shareh discoloration, to establish a multilocus sequence analysis method, to identify its evolutionary characteristics, and to provide evidence for clinical use of antimicrobial agents and prevention and control of this pathogen infection. Methods 40 strains of Shareh discoloration isolated from September 2013 to December 2014 were tested for in vitro drug sensitivity and multisite sequence analysis (MLSA),) and bioinformatics analysis with Splits tree and CLUSTALW software were carried out to reveal the epidemic trend. Results the results of in vitro drug sensitivity test showed that the resistance rate of 40 strains of Shareh discoloration to 13 antimicrobial agents was 10.0% and 32.5%, respectively. The resistance rate to imipenem was 32.5% and 4 housekeeping genes were designed, and the GC content was about 60.0%. The number of alleles was 14 / 16, and the number of polymorphic loci was the highest among the polymorphic loci of 3374gyrB (74); Splits tree software cleavage analysis showed that the majority of Shareh discoloring bacteria gathered together to form a clone complex; The results of cluster analysis by CLUSTALW software showed that 40 strains of Shareh discoloration bacteria formed 19 ST type, of which 12 were ST8 type, and ST8 type was the epidemic and violent hairstyle of Shareh bacillus resistant to carbapenem antibiotics. Formation of a clone complex CC8. by ST10 and ST11 Conclusion the resistance rate of Shareh discoloration to carbapenem antibiotics is high, and most of them are pan-resistant strains. The molecular epidemiological trend of Shareh fading bacteria is relatively slow and highly conserved in the genome. It is necessary to monitor the prevalence of the CC8 clone complex of Shareh discoloring bacteria represented by ST8 type in order to prevent its large-scale epidemic and outbreak in hospitals.
【作者单位】：苏州大学附属第一医院检验科;
【基金】：国家自然科学青年基金资助项目(81501425) 江苏省卫生厅医学科研基金资助项目(Q201401) 江苏省研究生培养创新工程基金资助项目(KYLX-1261) 苏州市“科教兴卫”青年基金资助项目(kjxw2014008)
【分类号】：R446.5;R440

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