运动干预对实验性高同型半胱氨酸血症大鼠过氧化及血管内皮功能的影响

发布时间：2018-12-06 08:35

【摘要】：目的:探讨运动干预对实验性高同型半胱氨酸血症(hyperhomocysteinemia,HHcy)大鼠过氧化及血管内皮功能的影响。方法:将48只大鼠随机分为6组,每组各8只。对照组(n=8只)给予普通饲料喂养;高蛋氨酸组(n=40只)在此基础上加3%的蛋氨酸,再根据运动强度分为模型组、叶酸组、HHcy运动组(低强度组、中强度组、高强度组),8只/组,持续16周。检测各组大鼠血清同型半胱氨酸(Hcy)、血浆丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性,测定一氧化氮(NO)、一氧化氮合酶(NOS)、内皮素1(ET-1)水平。结果:干预后8周,HHcy运动组血清Hcy水平明显下降,且显著低于模型组(16.09±4.85 vs.24.24±3.11μmol/L)(P0.05);干预后16周,HHcy运动组血清Hcy水平与对照组无显著性差异(7.81±0.64 vs.6.63±1.27μmol/L)(P0.05),各组血清Hcy水平依次为叶酸组(6.63±1.27μmol/L)低强度组(7.62±0.73μmol/L)中强度组(6.91±0.37μmol/L)高强度组(6.19±0.34μmol/L)(P0.05);干预后16周,HHcy运动组MDA均显著下降,SOD活力和GSH-PX含量明显升高(P0.05),中、高强度运动组各过氧化指标与对照组无显著性差异(P0.05);与对照组比较,干预后16周模型组、叶酸组和低强度组ET-1明显升高(61.64±12.29、58.86±10.32、59.11±9.09 vs 49.48±11.09pg/ml),NOS(8.52±2.09、18.13±4.65、17.08±4.20 vs 33.98±6.67U/ml)、NO(1.19±0.24、1.30±0.41、1.30±0.41 vs 2.29±0.28μg/L)明显下降(P0.05),而中强度组、高强度组中ET-1、NOS与对照组比较,差异无显著性意义(P0.05);HHcy运动组、叶酸组NO均明显高于模型组(P0.05)。结论:高蛋氨酸饮食可诱发大鼠HHcy,而中、高强度运动干预有助于降低HHcy大鼠血清Hcy水平,改善氧化应激状态,减轻内皮功能损伤。
[Abstract]:Aim: to investigate the effects of exercise intervention on peroxidation and vascular endothelial function in experimental hyperhomocysteinemia (hyperhomocysteinemia,HHcy) rats. Methods: 48 rats were randomly divided into 6 groups with 8 rats in each group. The control group (n = 8) was fed with common feed. High methionine group (n = 40) added 3% methionine on this basis. According to exercise intensity, it was divided into three groups: model group, folic acid group, HHcy exercise group (low intensity group, moderate intensity group, high intensity group), 8 rats in each group for 16 weeks. The contents of plasma malondialdehyde (MDA), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and nitric oxide (NO), nitric oxide synthase (NOS),) were measured in serum homocysteine (Hcy), of rats in each group. Endothelin 1 (ET-1) level. Results: at 8 weeks after intervention, the serum Hcy level in HHcy exercise group was significantly lower than that in model group (16.09 卤4.85 vs.24.24 卤3.11 渭 mol/L) (P0.05). At 16 weeks after intervention, there was no significant difference in serum Hcy level between HHcy exercise group and control group (7.81 卤0.64 vs.6.63 卤1.27 渭 mol/L) (P0.05). The serum Hcy levels of folic acid group (6.63 卤1.27 渭 mol/L), low intensity group (7.62 卤0.73 渭 mol/L), moderate intensity group (6.91 卤0.37 渭 mol/L), high intensity group (6.19 卤0.34 渭 mol/L) (P0.05); At 16 weeks after intervention, MDA decreased significantly in HHcy exercise group, SOD activity and GSH-PX content increased significantly (P0.05), and there was no significant difference in peroxidation index between high intensity exercise group and control group (P0.05). Compared with the control group, ET-1 in the folic acid group and low intensity group increased significantly (61.64 卤12.2958.86 卤10.32 vs 59.11 卤9.09 vs 49.48 卤11.09pg/ml) 16 weeks after intervention. NOS (8.52 卤2.09 卤4.65 卤17.08 卤4.20 vs 33.98 卤0.24 6.67U/ml), NO (1.30 卤0.41 vs 2.29 卤0.28 渭 g / L) decreased significantly (P0.05), while in the medium intensity group, ET-1, in the high intensity group decreased significantly (P0.05). There was no significant difference between NOS and control group (P0.05). NO in HHcy exercise group and folic acid group were significantly higher than that in model group (P0.05). Conclusion: high methionine diet can induce HHcy, in rats. High intensity exercise intervention can reduce the level of serum Hcy in HHcy rats, improve the state of oxidative stress, and alleviate the injury of endothelial function.
【作者单位】：浙江医院心脏康复中心;
【基金】：浙江省自然科学基金资助项目(LY12H17001)
【分类号】：R54;R493

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