Carba NP法对产碳青霉烯酶菌株快速检测的研究

发布时间：2018-12-16 18:13

【摘要】：目的探讨生化试验Carba NP法用于产碳青霉烯酶肠杆菌属和假单胞菌属细菌的快速检测,以及鉴别区分产碳青霉烯酶A、B、D类型的准确程度与适用性。方法对2014年10月-2015年3月从医院临床标本分离的23株亚胺培南耐药菌株进行Carba NP法试验,并将阳性结果与PCR结果进行比对。结果 Carba NP法检测的23株胺培南耐药菌株中4株显色结果呈阳性,说明其产生碳青霉烯酶,并可将其进一步区分为2株A型、2株B型;对该4株耐药菌进行PCR检测产碳青霉烯酶基因,1株含基因VIM,1株含基因NDM(B型),2株含基因KPC(A型),该结果与Carba NP法结果相符。结论 Carba NP法简单易行、成本低廉、快速方便,特异性和灵敏度均较高,不仅能快速检测出产碳青霉烯酶菌株,并且能进一步判定产碳青霉烯酶菌株的类型,节省了检测时间,有助于提高产碳青霉烯酶菌株的检出率,结合PCR及测序能有效控制医院获得性感染。
[Abstract]:Objective to study the rapid detection of carbapenem producing Enterobacter spp and Pseudomonas by biochemical test Carba NP, and to identify the accuracy and applicability of the identification and differentiation of carbapeninase Acarbendase D type. Methods from October 2014 to March 2015, 23 strains of imipenem resistant strains were tested by Carba NP, and the positive results were compared with the results of PCR. Results four of the 23 amponem-resistant strains detected by Carba NP showed positive staining, which indicated that they produced carbapenem, and could be further divided into two strains of type A and two strains of type B. The four strains were tested for carbapenase gene production by PCR, one strain containing gene VIM,1 strain containing gene NDM (B type, and two strains containing gene KPC (A type). The results were consistent with Carba NP method. Conclusion Carba NP method is simple, cheap, rapid, convenient, specific and sensitive. It can not only detect carbapenem producing strains quickly, but also further determine the types of carbapenem producing strains, thus saving the detection time. It is helpful to improve the detection rate of carbapenem producing strains. Combined with PCR and sequencing, nosocomial infection can be effectively controlled.
【作者单位】：天津市第三中心医院检验科;
【基金】：天津市卫生局科技基金资助项目(2013KY02)
【分类号】：R446.5

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