肺炎克雷伯菌分离株的耐药性与毒力基因分布及相互关系研究

发布时间：2018-12-17 05:17

【摘要】：目的研究肺炎克雷伯菌的耐药性与毒力基因的分布及二者间的关系,为临床合理使用抗菌药物和控制感染提供依据。方法分离出172株肺炎克雷伯菌,采用纸片扩散法(K-B法)做药物敏感试验;聚合酶链反应(PCR)检测rmpA、aer-1、aer-2、magA、gyrB-2、ybt、wcaG毒力基因。结果 172株肺炎克雷伯菌分离株对头孢菌素类头孢唑啉、头孢呋辛、头孢噻肟与头孢曲松的耐药率分别为38.4%、34.9%、33.1%、32.0%。rmpA、aer-1、aer-2、magA、gyrB-2、ybt、wcaG毒力基因的阳性率分别为49.4%、55.8%、42.4%、16.3%、51.7%、53.5%、20.9%。肺炎克雷伯菌毒力基因分布与耐药性呈负相关,差异有统计学意义(P0.01)。结论肺炎克雷伯菌毒力基因数与耐药性密切相关,临床应注意抗菌药物的合理应用,防止肺炎克雷伯菌耐药性的产生和传播。
[Abstract]:Objective to study the relationship between drug resistance and virulence gene distribution of Klebsiella pneumoniae and to provide evidence for rational use of antimicrobial agents and infection control. Methods 172 strains of Klebsiella pneumoniae were isolated, and the susceptibility test was performed by disk diffusion method (K-B), and the virulence gene of rmpA,aer-1,aer-2,magA,gyrB-2,ybt,wcaG was detected by polymerase chain reaction (PCR). Results the resistance rates of 172 Klebsiella pneumoniae isolates to cephalosporin-like cefazolin, cefuroxime, cefotaxime and ceftriaxone were 38.4% and 38.9% respectively. The positive rates of gyrB-2,ybt,wcaG virulence genes were 49.4 and 42.4, respectively. The distribution of virulence gene of Klebsiella pneumoniae was negatively correlated with drug resistance (P0.01). Conclusion the number of virulence genes of Klebsiella pneumoniae is closely related to drug resistance. The rational use of antimicrobial agents should be paid attention to in order to prevent the emergence and spread of drug resistance of Klebsiella pneumoniae.
【作者单位】：苏州大学附属第一医院检验科;山东省日照市人民医院检验科;
【基金】：江苏省卫生厅医学科研项目(Q201401) 江苏省研究生培养创新工程项目(KYLX-1261) 苏州市“科教兴卫”青年课题项目(kjxw2014008)
【分类号】：R440

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