感染性病毒PMA联合qPCR定量检测

发布时间：2018-12-18 17:25

【摘要】：细胞培养作为定量检测活病毒的金标准,耗时长,通常需要数天到数周,光敏染料叠氮溴化丙锭联合qPCR(PMA-qPCR)作为一种新型定量检测病毒感染性的分析工具,具有快速便捷的优点PMA预处理待测样品,选择性渗入灭活病毒衣壳,结合核酸后抑制其扩增,使得仅活病毒核酸可以进行qPCR本研究就PMA-qPCR检测活病毒的机理、灭活病毒的方法进行分析讨论,并比较不同方案检测病毒感染性的差异性。
[Abstract]:Cell culture, as a gold standard for quantitative detection of live viruses, takes a long time and usually takes several days to weeks. Guang Min dye azide and qPCR (PMA-qPCR) are used as a new analytical tool for quantitative detection of viral infection. PMA has the advantages of fast and convenient to pretreat the samples to be tested, selectively infiltrate the inactivated virus capsid, combine with nucleic acid to inhibit its amplification, so that only live virus nucleic acid can be carried out qPCR this study on the mechanism of PMA-qPCR detection of live virus. The methods of inactivation of virus were analyzed and discussed, and the differences of different methods for virus infection detection were compared.
【作者单位】：南方医科大学公共卫生学院;南方医科大学第二临床医学院;
【基金】：国家自然科学基金(31270974;31470271) NSFC-广东联合基金(U1132002) 广东省省级科技计划项目(2013A020229004) 广州市科技计划项目(201508020263)
【分类号】：R440

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