解脲支原体金标免疫层析检测试剂盒的研制
发布时间：2019-02-28 12:12
【摘要】：目的研制一种解脲支原体(Uu)胶体金免疫层析试剂盒,用于临床快速检测、流行病学调查。方法原核表达解脲支原体MB(Uu-MB)蛋白,制备Uu-MB单抗后胶体金标记,采用双抗体夹心法制备试剂盒,评价试剂盒的特异性、敏感性、稳定性及符合率。结果对Uu阳性培养物和Uu-MB具有良好特异性;37℃放置12d质量稳定;试剂盒与(Uu)支原体分离鉴别管的检测阳性率分别为29.59%和71.42%;Uu金标检测试剂盒与Uu分离鉴别管的阳性、阴性符合率为41.28%、100.0%;Uu分离鉴别管培养物经PCR鉴定,两者的阳性、阴性符合率为100%、75.65%。结论研制的试剂盒操作简便,具有特异性强、敏感性高等特点,是一种适宜普及推广的Uu感染检测产品。
[Abstract]:Objective to develop a gold immunochromatographic kit for rapid detection and epidemiological investigation of Ureaplasma Urealyticum (Uu) colloid. Methods Ureaplasma Urealyticum MB (Uu-MB) protein was expressed in E. coli and labeled with colloidal gold after preparation of Uu-MB monoclonal antibody. The kit was prepared by double antibody sandwich method. The specificity, sensitivity, stability and coincidence rate of the kit were evaluated. Results there was a good specificity for Uu positive culture and Uu-MB, the quality was stable at 37 鈩，
本文编号：2431792
[Abstract]:Objective to develop a gold immunochromatographic kit for rapid detection and epidemiological investigation of Ureaplasma Urealyticum (Uu) colloid. Methods Ureaplasma Urealyticum MB (Uu-MB) protein was expressed in E. coli and labeled with colloidal gold after preparation of Uu-MB monoclonal antibody. The kit was prepared by double antibody sandwich method. The specificity, sensitivity, stability and coincidence rate of the kit were evaluated. Results there was a good specificity for Uu positive culture and Uu-MB, the quality was stable at 37 鈩，
本文编号：2431792
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