侵袭性感染金黄色葡萄球菌的毒力基因系统研究

发布时间：2019-03-14 10:21

【摘要】：目的研究侵袭性感染金黄色葡萄球菌分离株的毒力基因存在状况,为临床治疗提供参考依据。方法收集杭州市余杭区中医院2013年2-7月20株金黄色葡萄球菌侵袭性感染临床分离株,采用聚合酶链反应(PCR)的方法,分析共6大类94种毒力基因;并对毒力基因检测结果作菌株系统发育分析,数据采用SPSS 17.0软件进行统计分析。结果 20株侵袭性感染金黄色葡萄球菌中共检测到66种毒力基因:包括11种黏附毒素毒力基因、6种细胞毒素毒力基因、3种侵袭性酶毒力基因、12种超抗原毒力基因、32种外毒素毒力基因、2种荚膜抗原毒力基因;系统发育分析结果为20株金黄色葡萄球菌可分为A、B两个簇群,其中B簇群中的11、14号株和2、3、19号株可能为克隆播散。结论侵袭性感染金黄色葡萄球菌携带多种毒力基因,具有极强的侵袭性和定植能力,对人体致病性强,对金黄色葡萄球菌人体侵袭性感染分离株进行黏附毒素、细胞毒素、侵袭性酶、超抗原、外毒素、荚膜抗原等六大类94种毒力基因检测研究为国内首次。
[Abstract]:Objective to study the existence of virulence genes in invasive Staphylococcus aureus isolates and to provide reference for clinical treatment. Methods from February to July 2013, 20 clinical isolates of invasive infection of Staphylococcus aureus were collected from Yuhang Hospital, Hangzhou City, and 94 virulence genes of 6 categories were analyzed by polymerase chain reaction (PCR). The results of virulence gene detection were analyzed by phylogenetic analysis. The data were statistically analyzed by SPSS 17.0 software. Results 66 virulence genes were detected in 20 strains of invasive Staphylococcus aureus, including 11 virulence genes of adhesion toxin, 6 virulence genes of cytotoxin, 3 virulence genes of invasiveness enzyme and 12 virulence genes of superantigen. 32 exotoxin virulence genes, 2 capsular antigen virulence genes; Phylogenetic analysis showed that 20 strains of Staphylococcus aureus could be divided into A and B clusters, of which 11, 14 and 2,3, 19 strains were probably cloned and disseminated. Conclusion Staphylococcus aureus with invasive infection has many virulence genes, it has strong invasiveness and colonization ability, and has strong pathogenicity to human body. It can be used as adhesion toxin and cytotoxin to human invasive infection strains of Staphylococcus aureus. Invasive enzyme, superantigen, exotoxin, capsule antigen and other 94 virulence genes were detected for the first time in China.
【作者单位】：杭州市余杭区中医院检验科;宁波市第一医院检验科;浙江大学医学院附属第一医院传染病诊治国家重点实验室;
【基金】：浙江省教育厅科研基金资助项目(Y201327731) 杭州市科技发展计划基金资助项目(20140633B56)
【分类号】：R446.5

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