不同亚抑菌浓度头孢他啶抑制大肠埃希菌生物膜形成的量效分析

发布时间：2019-05-08 23:42

【摘要】：目的分析亚抑菌浓度头孢他啶在抑制临床分离大肠埃希菌株生物膜形成过程中的浓度变化以及其作用机制。方法根据CLSI倍比稀释法检测大肠埃希菌临床耐药株E46对15种抗生素的最低抑菌浓度(minimum inhibitory concentration,MIC),通过PCR检测其超广谱β-内酰胺酶(superspectrumβ-lactamase,ESBLs)相关基因的表达,采用高效液相色谱法(HPLC)分析头孢他啶在抑制E46生物膜形成过程中药物的浓度变化。结果 E46仅对亚胺培南、美罗培南、阿米卡星、呋喃妥因敏感,对头孢他啶等9种抗菌药物耐药,头孢他啶在1/16~1/2 MIC范围内呈剂量依赖性地抑制E46生物膜的形成(P0.05),E46携带TEM和CTX-M-15型酶,在对E46生物膜的抑制过程中,头孢他啶的浓度逐渐降低。结论亚抑菌浓度的头孢他啶可以抑制大肠埃希菌临床耐药株E46生物膜的形成,但其作用浓度随时间延长降低显著。
[Abstract]:Aim to analyze the concentration changes of ceftazidime in inhibiting the biofilm formation of clinically isolated Escherichia coli strains and the mechanism of Ceftazidime in inhibiting biofilm formation of clinically isolated Escherichia coli strains. Methods the minimal inhibitory concentration (minimum inhibitory concentration,MIC) of clinical resistant Escherichia coli strain E46 to 15 antibiotics was detected by CLSI dilution method, and the expression of extended spectrum 尾-lactamase (superspectrum 尾-lactamase,ESBLs) related gene was detected by PCR. The concentration changes of ceftazidime during the inhibition of E46 biofilm formation were analyzed by high performance liquid chromatography (HPLC). Results E46 was only sensitive to imipenem, meropenem, amikacin, furantoin, and resistant to ceftazidime. Ceftazidime inhibited the formation of E46 biomembrane in a dose-dependent manner within the range of 1 ~ 16 ~ 1 ~ 2 MIC (P0.05). The concentration of ceftazidime decreased gradually during the inhibition of E46 biofilm by carrying TEM and CTX-M- 15 enzymes. Conclusion Ceftazidime with sub-inhibitory concentration could inhibit the formation of E46 biofilm, but the concentration of ceftazidime decreased significantly with the prolongation of time.
【作者单位】：第三军医大学西南医院药剂科;
【基金】：国家自然科学基金面上项目(81373451)~~
【分类号】：R446.5

【相似文献】