可持续建筑材料中的生物矿化研究
发布时间：2021-01-31 11:09
伴随着我国的城市化进程加快,建筑行业迅速发展。混凝土成为了世界上用途最广、用量最大的建筑材料,而其主要成分的水泥并不是一种可持续发展的胶结材料。每生产1吨水泥就会排放近1吨CO2,严重影响了城市的生态环境。我国已把推进建筑业可持续发展作为节能减排的重要内容,寻找生态环境材料是发展低碳建筑的内在要求。而自然的生态智慧可用于解决建筑材料与维护生态环境之间的矛盾。许多天然生物材料,比如珊瑚、骨骼、牙齿和贝壳等,是生物矿化过程中形成的钙质陶瓷和高分子复合物。这为建筑材料的可持续发展提供了一种新思路。某些微生物可用于模拟自然界的矿化过程,产生具有胶结作用的碳酸钙沉积。这类微生物主要是产脲酶菌,其矿化产物被称为“生物水泥”,这一生物矿化过程也被称为Microbially Induced calcium Carbonate Precipitation（MICP）。本文围绕基于MICP的生物矿化过程在可持续建筑材料中的应用潜力这一中心问题,研究了生物水泥修复受损建筑材料以延长服役寿命,探索了生物水泥与辅助性胶凝材料（偏高岭土、粉煤灰）复合用于降低水泥基材料中水泥的含量以及改良土体...
【文章来源】：华东师范大学上海市 211工程院校 985工程院校 教育部直属院校
【文章页数】：156 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Abbreviations
Chapter 1: Introduction
1.1 General Introduction
1.2 Ecological wisdom in nature for sustainable building materials
1.3 Microbially induced calcium carbonate precipitation
1.4 Problem and gap in studies
1.5 Research objectives
1.6 Thesis organization
Chapter 2: Literature Review
2.1 Microbial activities leading to carbonate precipitation
2.2 MICP process driven by urease enzyme
2.3 Factors affecting the efficiency of MICP
2.4 Urease producing bacterial isolation source
2.5 Polymorphism of carbonate crystals
2.6 Production of MICP: Biocement
2.7 Biocement and properties of building materials
2.8 Applications of MICP in building materials
2.8.1 Biocement in remediation of building materials
2.8.2 Biocement in low energy building materials
2.8.3 MICP in ground improvement
2.9 Summary of literature review and future prospective
Chapter 3: Complete bacterial community analysis of Yixing Shanjuan Cave and bio-consolidation of cracks in masonry cement mortars by one of urease producing isolate
3.1 Introduction
3.2 Materials and methods
3.2.1 Sample collection
3.2.2 Bacterial community analysis using Illumina Mi Seq
3.2.2.1 Total DNA extraction and DNA sequence analysis
3.2.2.2 Bioinformatics analysis
3.2.3 Isolation and characterization analysis
3.2.3.1 Bacterial isolation
3.2.3.2 Identification of best urease producing bacteria
3.2.3.3 Optimization of conditions for urease activity
3.2.3.4 Bacterial growth profile and p H profile
3.2.3.5 Urease activity
3.2.3.6 Calcite estimation
3.2.4 Bio-consolidation of cracks in masonry cement mortars
3.2.4.1 Biocement production
3.2.4.2 Mortar preparation and crack generation
3.2.4.3 Consolidation of cracks
3.2.4.4 Water absorption
3.2.4.5 Compressive strength
3.2.4.6 Micro-structural analyses
3.2.4.7 Thermogravimetric and differential scanning calorimetry
3.3 Results and Discussion
3.3.1 Microbial diversity of Yixing Shanjuan karst cave of China
3.3.2 Isolation and characterization analysis
3.3.2.1 Isolation and identification of best ureolytic isolate
3.3.2.2 Optimization of conditions for urease activity
3.3.2.3 Bacterial growth and p H profiles
3.3.2.4 Urease activity and calcite estimation
3.3.3 Bio-consolidation of cracks in masonry cement mortars
3.3.3.1 Compressive strength
3.3.3.2 Water absorption
3.3.3.3 Micro-structural analyses
3.3.3.4 Thermogravimetric analysis
3.4 Conclusion
Chapter 4: Improvement in the performance and properties of cement mortars with secondary cementitious material by biomineralization
4.1 Introduction
4.2 Materials and methods
4.2.1 Sample collection
4.2.2 Isolation and identification of urease producing bacterium
4.2.3 Materials
4.2.4 Biocement development
4.2.5 Biomineralization in MK
4.2.6 Mortar specimens preparation with MK
4.2.7 Porosity of mortar specimens
4.2.8 Micro-structural analyses
4.3 Results and discussion
4.3.1 Urease producing bacterium
4.3.2 Compressive strength
4.3.3 Porosity
4.3.4 SEM-EDS
4.3.5 FTIR
4.3.6 XRD
4.4 Conclusions
Chapter 5: Fly ash incorporated with biocement to improve engineering properties of expansive soil
5.1 Introduction
5.2 Materials and Methods
5.2.1 Materials
5.2.2 Biocement production
5.2.3 Sample preparation
5.2.4 Atterberg limits
5.2.5 Free swell testing method
5.2.6 Unconfined Compressive Strength （UCS） test
5.2.7 Micro-structural analyses
5.3 Results and Discussion
5.3.1 Atterberg limits
5.3.2 Swelling potential
5.3.3 Unconfined Compressive Strength （UCS）
5.3.4 SEM-EDX
5.3.5 FTIR and XRD
5.4 Conclusions
Chapter 6: Bio-grout based on microbially induced sand solidification by means of asparaginase activity
6.1 Introduction
6.2 Materials and Methods
6.2.1 Materials
6.2.2 Asparaginase assay
6.2.3 Bio-grout preparation
6.2.4 Strength and permeability of Bio-grout
6.2.5 Micro-structural analyses
6.2.6 X-ray computed tomography （XCT）
6.2.7 Thermogravimetry analysis （TGA）
6.3 Results
6.3.1 Asparaginase activity
6.3.2 Mechanical properties
6.3.3 SEM-EDS analysis
6.3.4 XRD and XCT
6.3.5 Thermogravimetric analysis （TGA）
6.4 Discussion
Chapter 7: Conclusion, Innovation and Future Perspectives
7.1 Conclusion
7.2 Innovation
7.3 Future perspectives
References
Appendix: Complete genome sequence of carbonic anhydrase producing Psychrobacter sp. SHUES
致谢
攻读博士学位期间发表的论文
本文编号：3010749
【文章来源】：华东师范大学上海市 211工程院校 985工程院校 教育部直属院校
【文章页数】：156 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Abbreviations
Chapter 1: Introduction
1.1 General Introduction
1.2 Ecological wisdom in nature for sustainable building materials
1.3 Microbially induced calcium carbonate precipitation
1.4 Problem and gap in studies
1.5 Research objectives
1.6 Thesis organization
Chapter 2: Literature Review
2.1 Microbial activities leading to carbonate precipitation
2.2 MICP process driven by urease enzyme
2.3 Factors affecting the efficiency of MICP
2.4 Urease producing bacterial isolation source
2.5 Polymorphism of carbonate crystals
2.6 Production of MICP: Biocement
2.7 Biocement and properties of building materials
2.8 Applications of MICP in building materials
2.8.1 Biocement in remediation of building materials
2.8.2 Biocement in low energy building materials
2.8.3 MICP in ground improvement
2.9 Summary of literature review and future prospective
Chapter 3: Complete bacterial community analysis of Yixing Shanjuan Cave and bio-consolidation of cracks in masonry cement mortars by one of urease producing isolate
3.1 Introduction
3.2 Materials and methods
3.2.1 Sample collection
3.2.2 Bacterial community analysis using Illumina Mi Seq
3.2.2.1 Total DNA extraction and DNA sequence analysis
3.2.2.2 Bioinformatics analysis
3.2.3 Isolation and characterization analysis
3.2.3.1 Bacterial isolation
3.2.3.2 Identification of best urease producing bacteria
3.2.3.3 Optimization of conditions for urease activity
3.2.3.4 Bacterial growth profile and p H profile
3.2.3.5 Urease activity
3.2.3.6 Calcite estimation
3.2.4 Bio-consolidation of cracks in masonry cement mortars
3.2.4.1 Biocement production
3.2.4.2 Mortar preparation and crack generation
3.2.4.3 Consolidation of cracks
3.2.4.4 Water absorption
3.2.4.5 Compressive strength
3.2.4.6 Micro-structural analyses
3.2.4.7 Thermogravimetric and differential scanning calorimetry
3.3 Results and Discussion
3.3.1 Microbial diversity of Yixing Shanjuan karst cave of China
3.3.2 Isolation and characterization analysis
3.3.2.1 Isolation and identification of best ureolytic isolate
3.3.2.2 Optimization of conditions for urease activity
3.3.2.3 Bacterial growth and p H profiles
3.3.2.4 Urease activity and calcite estimation
3.3.3 Bio-consolidation of cracks in masonry cement mortars
3.3.3.1 Compressive strength
3.3.3.2 Water absorption
3.3.3.3 Micro-structural analyses
3.3.3.4 Thermogravimetric analysis
3.4 Conclusion
Chapter 4: Improvement in the performance and properties of cement mortars with secondary cementitious material by biomineralization
4.1 Introduction
4.2 Materials and methods
4.2.1 Sample collection
4.2.2 Isolation and identification of urease producing bacterium
4.2.3 Materials
4.2.4 Biocement development
4.2.5 Biomineralization in MK
4.2.6 Mortar specimens preparation with MK
4.2.7 Porosity of mortar specimens
4.2.8 Micro-structural analyses
4.3 Results and discussion
4.3.1 Urease producing bacterium
4.3.2 Compressive strength
4.3.3 Porosity
4.3.4 SEM-EDS
4.3.5 FTIR
4.3.6 XRD
4.4 Conclusions
Chapter 5: Fly ash incorporated with biocement to improve engineering properties of expansive soil
5.1 Introduction
5.2 Materials and Methods
5.2.1 Materials
5.2.2 Biocement production
5.2.3 Sample preparation
5.2.4 Atterberg limits
5.2.5 Free swell testing method
5.2.6 Unconfined Compressive Strength （UCS） test
5.2.7 Micro-structural analyses
5.3 Results and Discussion
5.3.1 Atterberg limits
5.3.2 Swelling potential
5.3.3 Unconfined Compressive Strength （UCS）
5.3.4 SEM-EDX
5.3.5 FTIR and XRD
5.4 Conclusions
Chapter 6: Bio-grout based on microbially induced sand solidification by means of asparaginase activity
6.1 Introduction
6.2 Materials and Methods
6.2.1 Materials
6.2.2 Asparaginase assay
6.2.3 Bio-grout preparation
6.2.4 Strength and permeability of Bio-grout
6.2.5 Micro-structural analyses
6.2.6 X-ray computed tomography （XCT）
6.2.7 Thermogravimetry analysis （TGA）
6.3 Results
6.3.1 Asparaginase activity
6.3.2 Mechanical properties
6.3.3 SEM-EDS analysis
6.3.4 XRD and XCT
6.3.5 Thermogravimetric analysis （TGA）
6.4 Discussion
Chapter 7: Conclusion, Innovation and Future Perspectives
7.1 Conclusion
7.2 Innovation
7.3 Future perspectives
References
Appendix: Complete genome sequence of carbonic anhydrase producing Psychrobacter sp. SHUES
致谢
攻读博士学位期间发表的论文
本文编号：3010749
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