氧化锌纳米晶合成表征及抗菌性能研究

发布时间：2018-01-03 23:19

本文关键词：氧化锌纳米晶合成表征及抗菌性能研究　出处：《内蒙古工业大学》2015年硕士论文　论文类型：学位论文

【摘要】：近几十年来,抗生素在医疗领域、养殖领域的大量过度使用,使本已严重的耐药问题日益恶化。超级细菌的出现更是为人们敲响了警钟,同时对抗菌制剂提出了更高的要求。目前科研人员开始转向寻找新型的抗耐药性强,广谱抗菌而且生物相容性良好的抗菌制剂。无机纳米材料作为一类新型抗菌剂,具有抗耐药性强、广谱抗菌等优势,近年来受到人们的普遍关注,其中,氧化锌作为无机纳米材料重要组成部分,在抗菌领域已经得到了一定的应用。然而,利用简单廉价的方法合成单分散性和生物相容性良好的小尺寸氧化锌纳米晶仍存在困难。同时,目前有关氧化锌纳米晶的抗菌应用多集中于体外研究,将其应用于体内抗菌的报道很少。本文利用小分子配体低温溶剂合成方法合成了小尺寸氧化锌,并首次对其生物安全性及其体内外抗菌活性进行了评价,主要内容包括:一、采用小分子配体低温溶剂热法合成水中稳定及单分散性良好的小尺寸氧化锌纳米晶。利用TEM,XRD等手段对其超微结构、尺寸、成分、表面性质及其光学性能进行表征评价。二、评价所制备氧化锌纳米晶对Hep G-2细胞的生物相容性及其导致细胞凋亡机理。同时考察氧化锌纳米晶在小鼠模型上的急性毒性表现,结果表明氧化锌纳米晶对Hep G-2细胞具有一定的毒性。纳米晶在高剂量时会导致小鼠死亡,但与先前报道相比,所制备氧化锌具有良好生物相容性和生物安全性。三、以大肠杆菌和金黄色葡萄球菌为检测模型,评价氧化锌纳米晶抑菌效果。抑菌圈实验和最小抑菌浓度(MIC)结果表明所制备氧化锌纳米晶拥有优良的抑菌效果。为了进一步评价氧化锌作为抗菌制剂在医学领域的潜在应用,本文以大肠杆菌诱导的小鼠阴道炎为模型,考察纳米氧化锌复合PVA对该感染模型的治疗效果。结果表明,氧化锌对大肠杆菌所引起的小鼠阴道炎具有良好的治疗效果,说明纳米氧化锌作为抗菌制剂在医学抗感染领域具有巨大的应用前景。综上所述,本文主要介绍了以一种崭新的方法合成小尺寸氧化锌纳米晶,并系统研究其生物相容性,抗菌活性及其在细菌抗感染模型上的应用,本文合成氧化锌纳米晶展示优越的抗菌性能和良好的生物相容性。这为纳米氧化锌进一步走向临床应用提供了重要的实验基础和理论指导。
[Abstract]:In recent decades, antibiotics in the medical field, a large number of breeding areas of excessive use, making the already serious problem of drug resistance is deteriorating. The super bacteria is a wake-up call for people at the same time, put forward higher requirements on antimicrobial agents. At present, researchers began to seek a new anti strong drug resistance, antibacterial good biocompatibility and antibacterial agents. The inorganic nano materials as a new type of antibacterial agent, anti drug resistance, antibacterial and other advantages, is widely concerned by people in recent years, including Zinc Oxide, as an important part of inorganic nano materials, has been widely used in the field of antimicrobial. However, the method is simple and cheap the synthesis of monodisperse nano crystalline Zinc Oxide compatible with small size and good biological difficulties still exist. At the same time, the antibacterial nano crystal with the Zinc Oxide focus In vitro study, its application in the in vivo antibacterial is rarely reported. In this paper, the small size of Zinc Oxide was synthesized by using small molecule ligands at low temperature solvent synthesis method, and for the first time on the biological safety and in vivo antibacterial activity was evaluated. The main contents include: first, using small molecule ligands low-temperature solvothermal synthesis and water stability good dispersion of small size Zinc Oxide nanocrystals. The use of TEM, XRD and other means on the ultrastructure, size, composition, characterization of surface properties and optical properties of the evaluation. Two, evaluation of the prepared nanocrystals of Hep Zinc Oxide G-2 cell biocompatibility and leads to apoptosis mechanism. At the same time to study acute toxicity of Zinc Oxide nanocrystals in a mouse model of performance, results show that Zinc Oxide nanocrystalline has certain toxicity to Hep cells. G-2 nanocrystals can lead to the death of mice in high dose, but with the previously reported Over, the prepared Zinc Oxide has compatibility and good biological safety. Biological three, Escherichia coli and Staphylococcus aureus as the detection model, to evaluate the effect of nano antibacterial. Zinc Oxide antibacterial assay and minimum inhibitory concentration (MIC) results indicated that the prepared nanocrystals have Zinc Oxide excellent antibacterial effect to. Further evaluation of Zinc Oxide as an antimicrobial potential application in the field of medicine preparation, mice taking vaginitis induced by Escherichia coli as a model to investigate the therapeutic effect of nano PVA on the Zinc Oxide composite infection model. The results showed that the mice caused by vaginitis Zinc Oxide on Escherichia coli has a good therapeutic effect, nano antibacterial agents has application prospect as Zinc Oxide giant in the field of anti infective medicine. To sum up, this paper mainly introduces a new method for the synthesis of small size nano Zinc Oxide Crystal, and study its biocompatibility, antibacterial activity and anti bacterial infection in the application model, this paper synthesized nanocrystals show Zinc Oxide compatibility and good antibacterial performance. The biological nano Zinc Oxide further clinical application provides an important experimental basis and theoretical guidance.

【学位授予单位】：内蒙古工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O614.241

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