静电纺丝法制备纳米羟基磷灰石基生物材料及其性能研究

发布时间：2018-01-12 13:21

本文关键词：静电纺丝法制备纳米羟基磷灰石基生物材料及其性能研究　出处：《暨南大学》2015年硕士论文　论文类型：学位论文

【摘要】：羟基磷灰石(hydroxyapatite,Ca5(PO4)3OH,Hap)拥有良好的生物相容性,以及优异的表面生物吸附活性,其在生物医学领域有着广泛的应用。至少一维上尺度小于100 nm的纳米Hap有着极佳生物学性能。本研究的目的是通过新型的方法合成的纳米Hap,并对其合成机理进行探讨。首先,通过水热法以柠檬酸钠与十六烷基三甲基溴化铵(CTAB)为调控剂,研究大小有机分子对Hap晶体形貌的影响。制备出了长200~500nm,宽20~50nm的片状Hap晶体;长约100~200 nm,直径约10~20 nm的细针状Hap晶体及长约为50~100 nm直径约为10~20nm的短棒状晶体。研究表明,CTAB(大分子)胶束为控制Hap晶体形貌的模板;小分子柠檬酸钠对Hap的结晶有抑制作用。当反应体系中同时存在CTAB与柠檬酸钠作为调控剂时,CTAB对Hap晶体的形貌起主要的模板作用。采用静电纺丝法,以磷灰石溶胶-凝胶前驱体复合高分子溶液制备出纤维直径在100nm以下的Hap纳米陶瓷纤维。纤维直径为80~110nm,由颗粒直径为20~40 nm的Hap晶粒聚集成多孔形态。调控Hap前驱体浓度与热处理温度可改变Hap纤维的形貌。Hap纤维直径随前驱体含量的增加而增加,随热处理温度的提高而减少;高前驱体浓度可导致Hap纤维形成多孔结构。以牛血清蛋白(BSA)为蛋白质模型药物,考察Hap纤维对BSA载药性能。Hap的载药量随纤维前驱体含量和热处理温度的升高而降低;BSA的缓释可持续18天以上。在Hap纤维对成骨细胞活性及ALP活性检测的分析中,Hap纤维表现出对成骨细胞的生长的抑制作用但同时对ALP表达的促进作用。通过静电纺丝法,将Ca P溶胶与聚(丙交酯-乙交酯)共聚物(PLGA)共混纺丝并进行原位矿化,探索一种有机无机复合材料的新型制备方法。成功制备了钙磷元素分布均匀的PLGA-Ca P前驱体共混复合纤维。Ca P溶胶的加入使纤维形貌发生了缠结、分支,纤维直径增大。之后通过氨化碱性处理,制备出Hap结晶度较低的PLGA/Hap复合纤维。经处理后,复合材料的力学性能提高,但断裂伸长率降低。细胞实验表明,PLGA/Hap复合纤维对于MC3T3前成骨细胞的生长与ALP表达的促进作用。
[Abstract]:Hydroxyapatite has good biocompatibility and excellent surface biosorption activity. It has been widely used in biomedical field. At least one dimensional nanometer Hap with upper scale less than 100nm has excellent biological properties. The purpose of this study is to synthesize nanometer Hap by a new method. Firstly, sodium citrate and cetyltrimethylammonium bromide (CTAB) were used as regulators by hydrothermal method. The effect of organic molecules on the morphology of Hap crystal was studied. A Hap crystal with a length of 200 ~ 500 nm and a width of 20 ~ 50 nm was prepared. The thin needle-like Hap crystals with a diameter of 10 ~ 20 nm and a short rod crystal with a diameter of about 50 ~ 100 nm and a diameter of about 10 ~ 20 nm were obtained. CTAB micelles are templates for controlling the morphology of Hap crystals. The crystallization of Hap was inhibited by small molecule sodium citrate. When CTAB and sodium citrate were used as regulators in the reaction system. CTAB plays an important role as template for the morphology of Hap crystal. Electrostatic spinning method is used. Hap nanoceramic fibers with fiber diameter less than 100nm were prepared from apatite sol-gel precursor composite polymer solution with the fiber diameter of 80,110nm. The diameter of the particle is 20 ~ 40. The morphology of Hap fiber can be changed by adjusting the concentration of Hap precursor and heat treatment temperature. The diameter of Hap fiber increases with the increase of precursor content. It decreases with the increase of heat treatment temperature. High concentration of precursor resulted in the formation of porous structure of Hap fibers. BSA was used as a protein model drug. The drug loading capacity of Hap fiber on BSA decreased with the increase of precursor content and heat treatment temperature. The sustained release of BSA lasted more than 18 days. In the analysis of the activity of Hap fiber to osteoblast and ALP activity. Hap fibers can inhibit the growth of osteoblasts but at the same time promote the expression of ALP. Capp sol and poly (lactide-glycolide) copolymer (PLGA) were blended and spun and in situ mineralized. A new preparation method of organic-inorganic composite material was explored. The PLGA-Ca P precursor blend fiber, Ca with uniform distribution of calcium and phosphorus, was successfully prepared. The morphology of the fibers was entangled by the addition of P sol. After the branching, the diameter of the fiber increased, and then the PLGA/Hap composite fiber with low crystallinity of Hap was prepared by ammoniation and alkaline treatment. After treatment, the mechanical properties of the composites were improved. But the elongation at break decreased. Cell experiments showed that PLGA / Hap composite fibers promoted the growth and ALP expression of MC3T3 preosteoblasts.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;TQ340.64

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