海藻酸钙复合多孔薄膜的制备及其电化学研究

发布时间：2018-01-15 09:43

本文关键词：海藻酸钙复合多孔薄膜的制备及其电化学研究　出处：《青岛大学》2015年硕士论文　论文类型：学位论文

【摘要】：对第三代新型电化学生物传感器的构造及优化是本论文研究的主要内容,选用合适的新材料用于构造更加新颖且实用的电化学生物传感界面,使蛋白质、酶在修饰电极表面的电化学方面的性质得以更好地研究。为此,本文在原有多孔海藻酸钙(CA)膜修饰电极的基础上通过引入新物质,探究了肌红蛋白(Mb)如何更加稳定的通过薄膜固定在电极表面,构造出一种含水率高、稳定性好、电化学性能优良的电化学生物传感器,并且通过一系列的表征和电化学测试对其进行研究。具体内容如下:具有良好成膜性的海藻酸钠(SA)结合制孔剂聚乙二醇(PEG)已成功制备出多孔CA膜,在此基础上,为了得到一种稳定性更好、含水率更高的薄膜,将聚乙烯醇(PVA)、甘油和明胶引入多孔CA膜,从而构造出CA’s复合多孔薄膜。将CA’s复合多孔薄膜涂布于玻碳电极上,然后将Mb固载于此复合多孔薄膜修饰电极便成功制备出一种具有良好电化学性能的Mb-CA’s复合多孔薄膜修饰电极。利用扫描电子显微镜(SEM)、紫外-可见吸收光谱(UV-vis)、交流阻抗(EIS)、循环伏安(CV)、方波伏安(SWV)、计时安培等方法分别对所构造的复合多孔薄膜进行表征和性能测定。UV-vis表明Mb在所构造的CA’s复合多孔薄膜中依然保持了其原有的二级结构。EIS数据反映出加入PVA、甘油、明胶三种新物质后,电荷传递电阻(Rct)明显下降。CV曲线中在㧟0.37 V(vs SCE)处出现了蛋白质血红素FeIII/II电对的可逆氧化还原峰。对SWV法得到的数据进行拟合获得了表观异相电子传递速率常数(ks)及氧化还原式电势(Eο?)。Mb-CA’s复合多孔薄膜修饰电极在催化氧气(O2)、过氧化氢(H2O2)和亚硝酸钠(NaNO2)时均表现出良好的电催化性能,稳定性好,灵敏度高,检测限低。
[Abstract]:The structure and optimization of the third generation new electrochemical biosensor is the main content of this paper. The suitable new materials are selected to construct a more novel and practical electrochemical biosensor interface to make protein. The electrochemical properties of enzyme on the surface of modified electrode can be better studied. Therefore, a new material was introduced into the modified electrode based on the original porous calcium alginate CAA membrane modified electrode. This paper explores how myoglobin Mb) is more stable to be fixed on the electrode surface through thin film to construct an electrochemical biosensor with high moisture content, good stability and excellent electrochemical performance. It was studied by a series of characterization and electrochemical tests. The main contents are as follows: sodium alginate with good film forming property combined with polyethylene glycol (PEG). Porous CA membranes have been successfully prepared. On this basis, in order to obtain a film with better stability and higher moisture content, polyvinyl alcohol PVA, glycerol and gelatin were introduced into porous CA membrane. The CA's composite porous film was constructed and the CA's composite porous film was coated on the glassy carbon electrode. Then a Mb-CA's composite porous film modified electrode with good electrochemical performance was successfully prepared by immobilization of Mb on the composite porous film modified electrode. UV-Vis absorption spectra, AC impedance, cyclic voltammetry (CV), square wave voltammetry (SWV). The composite porous films were characterized and characterized by chronoamperometric method. UV-vis showed that Mb still maintained its original secondary structure in the CA's composite porous films. EIS data reflect the addition of PVA. After glycerin, gelatin three new substances, charge transfer resistance (Rct) significantly decreased in the. CV curve in? 0.37 VX vs SCE). The reversible redox peak of protein heme FeIII/II pair appeared. The apparent heterogeneous electron transfer rate constant was obtained by fitting the data obtained by SWV method. And redox potential? The modified electrode has good electrocatalytic performance and good stability when it is used to catalyze O _ 2O _ 2 H _ 2O _ 2 (H _ 2O _ 2 H _ 2O _ 2) and Na _ 2O _ 2 (Na _ (2) O _ (2) O _ (2) O _ (2) H _ (2) O _ (2)). High sensitivity and low detection limit.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.2

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