多孔二氧化硅薄膜的制备及其在蛋白质分子印迹中的应用

发布时间：2018-01-18 11:01

本文关键词：多孔二氧化硅薄膜的制备及其在蛋白质分子印迹中的应用　出处：《华中农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：现行蛋白质的分析传感等几乎完全依赖于抗体系统,而抗体系统存在着制备繁琐、价格昂贵、结构不稳定、不能重复性使用等缺点。本论文设计了一种基于多孔纳米材料的蛋白质印迹新方法,将现行的蛋白质三维印迹的高结合容量与二维印迹的高质量传递、易与传感平台集成等优点有机结合,并以有机硅烷试剂为功能单体,采取接近于生理水相环境的溶胶凝胶技术,提供了一种新的基于分子印迹的稳定、廉价、通用的蛋白质分离与分析方法。本论文主要研究内容包括以下几个方面:1、溶胶凝胶法制备的二氧化硅薄膜性质研究,包括机械性能(硬度、模量和脆性)、表面形貌和膜厚:研究了溶胶组分和溶胶老化时间对其机械性能的影响,当DMDMOS与TMOS的摩尔比为1:1、H2O与TMOS的摩尔比为4:1且溶胶老化时间在16h左右时,所形成的二氧化硅薄膜具有较稳定的机械性能和良好的表面形貌;研究了溶胶浓度对薄膜厚度的影响,可以通过简单的稀释方法得到厚度在1200nm到160nm可调的自支撑二氧化硅薄膜。2、可转移、自支撑多孔二氧化硅薄膜的制备:结合垂直沉积法和溶胶凝胶技术,分别利用细菌和不同直径的聚苯乙烯微球(200nm,290nm,400nm)为模板,制备了孔径大小可调、孔的层数可调的大面积自支撑二氧化硅薄膜。3、蛋白质印迹薄膜的制备及性质研究:结合分子印迹技术、垂直沉积法和溶胶凝胶技术,以细胞色素C为模板分子,制备了蛋白质印迹薄膜,通过调节聚苯乙烯微米球的自组装密度、结合识别蛋白质时缓冲液的p H、调节印迹薄膜的厚度和层数来提高蛋白质印迹薄膜的结合容量与质量传递能力。
[Abstract]:The current protein analysis sensor almost completely depends on the antibody system, and the antibody system has the characteristics of complicated preparation, expensive price and unstable structure. In this paper, a new method of Western imprinting based on porous nanomaterials is designed to transfer the high binding capacity of 3D imprinting to the high quality of two-dimensional imprinting. It is easy to integrate with the sensing platform and organic silane reagent is used as functional monomer and the sol-gel technology which is close to the physiological water phase environment provides a new kind of stable and cheap molecular imprinting. The main contents of this thesis include the following aspects: 1. The properties of silica films prepared by sol-gel method, including mechanical properties (hardness, modulus and brittleness). Surface morphology and film thickness: the effects of sol composition and aging time on mechanical properties were studied when the molar ratio of DMDMOS to TMOS was 1: 1. When the molar ratio of H2O to TMOS is 4: 1 and the aging time of sol is about 16 h, the Sio _ 2 film has stable mechanical properties and good surface morphology. The influence of sol concentration on the film thickness was studied. The self-supporting silica thin film with adjustable thickness from 1200 nm to 160 nm can be obtained by simple dilution method, which can be transferred. Preparation of self-supporting porous silica thin films: by means of vertical deposition and sol-gel technique, bacteria and polystyrene microspheres with different diameters were used at 290nm, respectively. 400nm) as template, a large area self-supporting silica thin film with adjustable pore size and adjustable number of pore layers was prepared. The preparation and properties of Western imprinted films were studied: combined with molecular imprinting technology. Western imprinted films were prepared by vertical deposition and sol-gel technique using cytochrome C as template molecule. By adjusting the self-assembly density of polystyrene microspheres, the buffer pH of protein recognition was combined. The binding capacity and mass transfer capacity of Western imprinted films were improved by adjusting the thickness and the number of layers.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ127.2;TB383.2

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