聚合修饰磁性纳米粒子的合成、表征及对蛋白质分离方法学的研究

发布时间：2018-01-24 12:59

本文关键词： 血红素蛋白分离聚合修饰磁性粒子　出处：《北京化工大学》2015年硕士论文　论文类型：学位论文

【摘要】：在生命科学的领域中,对于蛋白质进行选择性分离和检测都具有重要的意义。磁性材料的引入使得蛋白质的分离技术发展迅速,其快速高效的分离能力得到了研究者们的广泛青睐,越来越多的蛋白质分离研究倾向于选用磁性材料作为载体。通过磁性材料表面的修饰基团和蛋白质中的目标位点进行结合就可实现分离。本论文主要采用聚合方法对于磁性载体进行修饰并对其在蛋白质分离中的能力进行研究。在本论文研究中,目的是将磁性材料和聚合物材料的优势结合起来,合成一种高效分离血红素蛋白质材料。通过自由基聚合的方法,将具有咪唑环的涂层接枝在磁性粒子表面,通过咪唑环与血红素蛋白中铁的配位作用达到分离蛋白质的目的。通过一系列的表征对于所合成材料的结构和组成进行了研究,例如透射电子显微镜,傅里叶变换红外光谱和热重分析等。结果表明,聚乙烯基咪唑改性的磁性微米颗粒(PVIM-MMPs)在磁场中表现出强烈的磁响应,对于血红蛋白有较高的吸附能力(吸附量为6.96 g/g),较好的选择性(对比了非血红素蛋白质溶菌酶)和良好的可回收性。此外,研究中还制备了一种由聚乙烯基咪唑修饰的磁性纳米颗粒(PVIM-MNPs),并对其蛋白分离能力进行了考察,通过一系列的表征及测试,结果表明聚合物修饰的纳米颗粒表现出更好的吸附能力,对于未来生物大分子的分离载体提供了选择。综上,论文中建立的聚合方法所制备的材料对于血红素蛋白的分离具有选择性,分离方法简化了各种前处理,分离效率高,操作简便,可以快速实现磁性粒子的聚集并从母液分离,这一技术将有助于为蛋白质组学提供高丰度蛋白质去除技术,或低丰度蛋白质的富集研究,特别是针对血红素蛋白的分离纯化研究方面有很好的意义。
[Abstract]:In the field of life science, the selective separation and detection of proteins are of great significance. The introduction of magnetic materials makes the rapid development of protein separation technology. Its rapid and efficient separation ability has been widely favored by researchers. More and more researches on protein separation tend to use magnetic materials as carriers. The separation can be realized by binding the modified groups on the surface of magnetic materials and the target sites in proteins. Methods to modify the magnetic carrier and study its ability in protein separation. The aim of this study was to combine the advantages of magnetic materials with polymer materials to synthesize a highly efficient heme protein separation material. The coating with imidazole ring was grafted onto the surface of magnetic particles by free radical polymerization. The protein was isolated by the coordination between imidazole ring and iron in heme protein. The structure and composition of the synthesized materials were studied by a series of characterization, such as transmission electron microscopy. Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The results show that PVIM-MMPsmodified by polyvinylimidazolium has a strong magnetic response in the magnetic field. High adsorption capacity for hemoglobin (6.96 g / g), better selectivity (compared with non-heme protein lysozyme) and good recoverability. A kind of magnetic nanoparticles PVIM-MNPsN modified by polyethylene imidazole was prepared and its protein separation ability was investigated. A series of characterization and tests were carried out. The results show that polymer modified nanoparticles exhibit better adsorption ability and provide a choice for the separation of biomolecules in the future. The materials prepared by the polymerization method in this paper have selectivity for the separation of heme protein. The separation method simplifies all kinds of pretreatment, and the separation efficiency is high and the operation is simple. Magnetic particles can be rapidly aggregated and separated from mother liquor. This technique will be helpful to provide proteomics with high abundance protein removal technology or low abundance protein enrichment research. In particular, the study on the separation and purification of heme proteins is of great significance.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O631;TB383.1

【参考文献】