基于金属纳米簇的光学分析方法研究

发布时间：2018-01-27 01:10

本文关键词： 纳米簇化学发光 G-quadruplex-hemin DNAzyme 胰蛋白酶荧光聚胸腺嘧啶 L-半胱氨酸　出处：《陕西师范大学》2015年硕士论文　论文类型：学位论文

【摘要】：随着纳米科学的发展,纳米材料已被用于物理、化学、材料、生物、力学等研究领域。金属纳米簇是一种新型的荧光纳米材料。它具有尺寸小、稳定性好、荧光强、斯托克斯位移大、生物相容性好等优点,近年来在生物传感及细胞成像等方面有着广泛的应用。本论文着重对金纳米簇的化学发光性能以及铜纳米簇的荧光性能进行了研究,探索了金属纳米簇在生物传感中的新应用。本论文分为两章。第一章是综述,简单介绍了金属纳米簇的基本概念,总结了金属纳米簇的合成方法、光学特性以及在生物传感中的应用。第二章是研究报告,分为两个部分,具体内容如下：一、金纳米簇-过氧化氢-DNAzyme化学发光体系检测胰蛋白酶本研究工作将牛血清蛋白稳定的金纳米簇(BSA-Au NCs)引入化学发光,构建了Au NCs-H2O2-DNAzyme化学发光体系,考察了pH以及金属离子对体系化学发光强度的影响,探讨了体系的化学发光性质。基于胰蛋白酶可以消解金纳米簇的稳定剂牛血清蛋白(BSA),引起Au NCs-H2O2-DNAzyme体系化学发光强度减弱,由此建立了测定胰蛋白酶的化学发光新方法。该方法测定胰蛋白酶在1.0×10-m g/mL～1.0×10-7g/mL及1.0×10-7g/mL～1.0×10-5g/mL范围内呈现良好的线性关系,检出限为28 pg/mL。本法的灵敏度与文献报道的荧光法测定胰蛋白酶相比提高了71倍,表现出较好的应用前景。二、聚胸腺嘧啶寡核苷酸介导的铜纳米簇荧光探针测定L-半胱氨酸本研究工作将聚胸腺嘧啶寡核苷酸作为模板分子,利用一步法温和快速合成了稳定性好、荧光性能优异的铜纳米簇。L-半胱氨酸能通过巯基与铜形成Cu-S键,而与纳米簇发生相互作用,从而猝灭铜纳米簇的荧光。基于此现象,建立了测定L-半胱氨酸的荧光分析新方法。与已报道测定法相比,本方法无需任何洗涤、分离等操作步骤,操作简单方便,且整个实验过程在10分钟内即可完成,高效快速。本方法测定L-半胱氨酸的线性范围为3 μM-50μM,检出限为0.56 μM,已成功用于尿液中L-半胱氨酸含量的测定。
[Abstract]:With the development of nanoscience, nanomaterials have been used in physics, chemistry, materials, biology, mechanics and so on. Metal nanoclusters are a new kind of fluorescent nanomaterials with small size and good stability. It has the advantages of strong fluorescence, large Stokes shift and good biocompatibility. In recent years, it has been widely used in biosensor and cell imaging. In this paper, the chemiluminescence properties of gold nanoclusters and the fluorescence properties of copper nanoclusters have been studied. This paper is divided into two chapters. The first chapter is a review. The basic concepts of metal nanoclusters are briefly introduced, and the synthesis methods of metal nanoclusters are summarized. Optical properties and applications in biosensors. The second chapter is a study report, which is divided into two parts, the specific contents are as follows: 1. Determination of trypsin using gold nanoclusters-hydrogen peroxide -DNAzyme chemiluminescence system. In this study, BSA-Au NCs), a stable gold nanocluster with bovine serum protein, was introduced into chemiluminescence. Au NCs-H2O2-DNAzyme chemiluminescence system was constructed and the effects of pH and metal ions on the chemiluminescence intensity of the system were investigated. The chemiluminescence properties of the system were investigated. Bovine serum protein (BSA), a stabilizer based on trypsin, which could dissolve gold nanoclusters, was studied. The chemiluminescence intensity of au NCs-H2O2-DNAzyme system decreases. A new chemiluminescence method for the determination of trypsin was established, and the method was used to determine trypsin at 1.0 脳 10 ~ (-m) m. There is a good linear relationship between 1.0 脳 10 ~ (-7) g / mL and 1.0 脳 10 ~ (-7) g / mL. The detection limit is 28 PG / mL. The sensitivity of this method is 71 times higher than that of fluorescence method reported in literature. Determination of L- cysteine by fluorescent probe mediated by Polythymidine oligonucleotides Polythymine oligonucleotides were synthesized with one step method and rapid synthesis with good stability. Copper nanoclusters with excellent fluorescence properties. L- cysteine can form Cu-S bond with copper via sulfhydryl group and interact with nanoclusters to quench the fluorescence of copper nanoclusters. A new fluorescence analysis method for the determination of L-cysteine was established. Compared with the reported method, the method has no need of washing, separation and other operation steps, and the operation is simple and convenient. The whole experiment can be completed in 10 minutes. The linear range of determination of L- cysteine is 3 渭 M-50 渭 M and the detection limit is 0.56 渭 M. It has been successfully applied to the determination of L-cysteine in urine.
【学位授予单位】：陕西师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O657.3;TB383.1

【相似文献】