含聚六亚甲基单胍的抗菌材料的制备与性能研究

发布时间：2018-01-31 15:20

本文关键词： 聚六亚甲基单胍紫外辐照抗菌水凝胶天然植物纤维生物相容性　出处：《浙江大学》2017年硕士论文　论文类型：学位论文

【摘要】：细菌感染已成为人类健康的主要威胁之一。抗生素被广泛用于各种细菌感染的治疗,但是抗生素的过量使用导致了耐药细菌的产生,并且对环境造成污染。因此,开发高效、安全的抗菌材料,在人体外抑制细菌的生长,减少对人体的伤害,具有非常重要的现实意义和广阔的应用前景。聚六亚甲基单胍作为一种新型环保型高分子杀菌消毒剂,具有广谱抗菌性能,且价格低廉、无毒无害。本论文采用商品化的聚六亚甲基单胍盐酸盐,通过化学改性法制备了具有长效抗菌性能的水凝胶和天然植物纤维,并详细研究了它们的物理化学性能。水凝胶是一种由物理或化学作用形成的具有特定三维网络结构的材料,在组织工程支架、伤口敷料、药物载体等生物医学领域被广泛应用。抗菌水凝胶能够避免或减少抗生素的使用,具有临床应用的潜力。本论文首先合成了可聚合的聚六亚甲基单胍大分子前体,然后通过光引发的自由基聚合制备了聚六亚甲基单胍-聚丙烯酰胺水凝胶。采用抑菌圈法及光密度值法表征了水凝胶的抑菌性能。由于聚六亚甲基单胍链段通过共价键结合于水凝胶的骨架上,经多次长时间浸泡洗涤后仍不会流失,该水凝胶对金黄色葡萄球菌和大肠杆菌具有良好的长效抗菌性能。此外,溶血实验、细胞存活率测试表明该抗菌水凝胶具有良好的生物相容性,有望成为一种新型生物医学材料。天然植物纤维来源丰富、价格低廉,且易于降解,是属于符合可持续发展要求的天然可再生资源,广泛应用于纺织品材料领域。织物在环境中易产生细菌感染问题,与人体接触时会对人体造成潜在危险。因此,对天然植物纤维进行化学改性,使其具有良好的抗菌性能,意义深远。本论文选取了两种天然植物纤维,即醋酸纤维和棉纱线,通过简单的水相接枝改性方法,制备了聚六亚甲基单胍接枝改性的抗菌天然植物纤维。抑菌圈法、光密度值法等抗菌实验表明其对金黄色葡萄球菌和大肠杆菌均具有良好的抗菌性能,有望成为新型纺织品材料。
[Abstract]:Bacterial infection has become one of the major threats to human health. Antibiotics are widely used in the treatment of various bacterial infections, but the excessive use of antibiotics leads to the production of drug-resistant bacteria. Therefore, the development of efficient and safe antimicrobial materials to inhibit the growth of bacteria outside the human body, reduce the harm to the human body. Poly (hexamethylene monoguanidine), as a new environmental protection macromolecule sterilizing disinfectant, has broad spectrum antibacterial properties and low price. In this paper, hydrogels and natural plant fibers with long-term antibacterial properties were prepared by chemical modification with commercial polyhexamethylene monoguanidine hydrochloride. The physical and chemical properties of hydrogels are studied in detail. Hydrogel is a kind of material with specific three-dimensional network structure formed by physical or chemical action, in tissue engineering scaffold, wound dressing. Drug carriers and other biomedical fields are widely used. Antibacterial hydrogels can avoid or reduce the use of antibiotics. In this paper, we first synthesized polymerizable polyhexamethylene monoguanidine macromolecular precursor. Then the polyhexamethylene monoguanidine polyacrylamide hydrogel was prepared by photo-initiated free radical polymerization. The bacteriostatic properties of the hydrogel were characterized by bacteriostasis method and optical density method. Due to the passage of polyhexamethylene-monoguanidine chain through the polyhexamethylene-monoguanidine chain. The covalent bond binds to the framework of the hydrogel. The hydrogel has good long-term antibacterial properties against Staphylococcus aureus and Escherichia coli. In addition, the hemolysis experiment was carried out. The cell viability test shows that the antibacterial hydrogel has good biocompatibility and is expected to be a new biomedical material. The natural plant fiber is rich in sources, low in price and easy to degrade. It is a natural renewable resource which meets the requirements of sustainable development. It is widely used in the field of textile materials. Fabric in the environment is prone to the problem of bacterial infection, and contact with human body will cause potential danger to human body. Chemical modification of natural plant fiber makes it have good antibacterial properties. In this paper, two kinds of natural plant fiber, acetate fiber and cotton yarn, are selected and modified by water grafting. The antimicrobial natural plant fiber modified by polyhexamethylene monoguanidine was prepared. The antibacterial experiments showed that it had good antibacterial properties against both Staphylococcus aureus and Escherichia coli. It is expected to become a new textile material.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB34

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