淀粉基植物复合膜材的制备工艺及性能研究

发布时间：2018-02-03 12:39

本文关键词： 木薯醋酸酯淀粉果胶卡拉胶膜材　出处：《聊城大学》2017年硕士论文　论文类型：学位论文

【摘要】：传统的空心胶囊是以动物胶-明胶为膜材,已经具有百年的历史。但随着医药品种的增加和技术的进步,明胶胶囊的弊端逐渐显现。近年来,寻找明胶替代品成为胶囊研究的热点,其中以植物来源的多糖类物质如海藻酸钠、变性淀粉作为膜材成为研究的重点。本文选用木薯醋酸酯淀粉、玉米淀粉、卡拉胶和果胶等天然植物材料,分别制备了普通型和pH依赖型植物复合膜材,研究了膜材性能以及对药物释放的影响。主要研究内容如下:1.卡拉胶植物淀粉复合膜的制备采用木薯醋酸酯淀粉、玉米淀粉和卡拉胶复配制备植物空心胶囊成膜材料。首先分别研究了木薯醋酸酯淀粉、玉米淀粉和卡拉胶的浓度-粘度-温度关系、溶解度、膨胀率等性能;基于差式扫描量热分析和成膜性分析确定了木薯变性淀粉、玉米淀粉和卡拉胶三种配比,测定三种膜的透光率、水蒸气透过率和吸湿率等性能。结果表明醋酸酯淀粉对膜各种参数影响显著,随着醋酸酯淀粉所占比例的增大,膜的吸湿性和水蒸气透过率随之增大。2.pH依赖型植物高分子膜的制备及性能研究选用pH敏感型的果胶与木薯醋酸酯变性淀粉与卡拉胶复配制备pH依赖型天然高分子膜,研究了膜材料的性能。以膜断裂伸长率作为评价指标,正交优化复合膜组分配比,并对复合膜进行了透光性、吸湿性和水蒸气透过等性能测试。结果表明,三种组分中卡拉胶对膜性能影响最大,包括断裂伸长率和水蒸气透过系数2个关键膜性能指标,果胶主要影响复合膜的透光率,最优的复合膜组方为卡拉胶3%,醋酸酯淀粉3%,果胶1%。3.pH依赖型植物基微胶囊的制备和体外释药研究为研究新型肠溶植物基微胶囊,实现消化道定位给药,以植物多糖变性淀粉、果胶和卡拉胶为基质,制备了微胶囊并考察了其性质、体外释药行为和安全性。选用果胶、木薯醋酸酯淀粉与卡拉胶为材料,制备了pH依赖型微胶囊。考察了微胶囊的红外吸收特征、溶解性能、显微结构和细胞毒性,并进一步研究了微胶囊的释药行为。结果表明:当微胶囊中不含果胶时,其在不同pH条件下均不溶解且没有药物释放;当基质中果胶含量为1%,pH在中性偏酸性时微胶囊的释药量最大,随着果胶浓度的增大微胶囊的释药量降低,3%果胶含量微胶囊几乎没有释药。细胞毒性结果表明微胶囊是安全无毒的。果胶是pH依赖型微胶囊的关键成分,通过调节果胶含量可以实现微胶囊在pH中性环境下的有效释药,实现药物在肠道的定位释放,提高药物的生物利用度。4.空心胶囊的质量评价和稳定性研究选用羟丙基淀粉空囊与明胶空心胶囊两种空囊,依照《中国药典》2015年版的要求进行性能测定。将两种胶囊分别装载酸碱两种药物,载药后对胶囊在高温、高湿、高管照条件下的稳定性及溶出度情况进行测定。结果表明,淀粉空囊与明胶空心胶囊的性能相当。
[Abstract]:Traditional hollow capsules are made of animal gelatin and gelatin, which has a history of 100 years. But with the increase of medicine variety and the progress of technology, the disadvantages of gelatin capsules appear gradually in recent years. Finding a substitute for gelatin has become the focus of the capsule research, in which plant derived polysaccharides such as sodium alginate, modified starch as membrane materials become the focus of the study. This paper selected cassava acetate starch, corn starch. Natural plant materials, such as carrageenan and pectin, were used to prepare common and pH dependent plant composite membranes, respectively. The main contents of the study were as follows: 1. Cassava acetate starch was used as the preparation of carrageenan plant starch composite membrane. Corn starch and carrageenan were mixed to prepare plant hollow capsule film-forming materials. Firstly, the concentration-viscosity temperature relationship, solubility and expansion rate of cassava acetate starch, corn starch and carrageenan were studied respectively. Based on differential scanning calorimetry and film forming analysis, the ratio of cassava modified starch, corn starch and carrageenan was determined, and the transmittance of the three films was measured. The results showed that the effect of acetate starch on the membrane parameters was significant with the increase of the proportion of starch acetate. Hygroscopicity and water vapor transmittance of the membranes increased. 2. Preparation and properties of pH-dependent plant polymer membranes; Pectin, cassava acetate modified starch and carrageenan were mixed with pH-sensitive pectin and cassava acetate modified starch and carrageenan to prepare pH dependent polymer membranes. Natural polymer membrane. The properties of the composite films were studied. The elongation at break was taken as the evaluation index, the distribution ratio of composite films was optimized by orthogonal method, and the transmittance, moisture absorption and water vapor permeability of the composite films were tested. Carrageenan had the greatest influence on the membrane properties, including the elongation at break and the water vapor transmittance. Pectin mainly affected the transmittance of the composite membrane. The optimum compound membrane was carrageenan 3, acetate starch 3and pectin 1.3.The preparation and in vitro release of pH-dependent plant-based microcapsules were used to study the new enteric-soluble plant-based microcapsules. Using plant polysaccharide modified starch pectin and carrageenan as the matrix microcapsules were prepared and their properties in vitro drug release behavior and safety were investigated. Cassava acetate starch and carrageenan were used as materials to prepare pH-dependent microcapsules. The infrared absorption, solubility, microstructure and cytotoxicity of microcapsules were investigated. The drug release behavior of microcapsules was further studied. The results showed that when pectin was not found in microcapsules, the microcapsules did not dissolve at different pH and there was no drug release. When the content of pectin in the matrix was 1% pH, the release amount of microcapsule was the largest, and the release amount of microcapsule decreased with the increase of pectin concentration. The results of cytotoxicity showed that microcapsules were safe and non-toxic. Pectin was a key component of pH dependent microcapsules. By adjusting the content of pectin, the effective release of microcapsules in pH neutral environment can be realized, and the drug release in the intestinal tract can be realized. Improve the bioavailability of drugs .4.The quality evaluation and stability of hollow capsules were studied by using hydroxypropyl starch empty capsules and gelatin hollow capsules. According to the requirements of the 2015 edition of the Chinese Pharmacopoeia, the two capsules were loaded with acid-base drugs respectively, and the capsules were loaded with high temperature and high humidity. The stability and dissolution of gelatin hollow capsules were measured under irradiation conditions. The results showed that the properties of the empty starch capsules were similar to those of the gelatin hollow capsules.
【学位授予单位】：聊城大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.2;TQ460.4

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