基于蛋白质的复合纳米粒子的制备及其生物医学应用

发布时间：2018-02-09 09:08

本文关键词： 复合纳米粒子荧光蛋白纳米方块介孔二氧化硅纳米粒子生物影像药物控释　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：复合纳米粒子是有两种或者两种以上的分子或纳米尺度的材料组成的纳米粒子,其往往有着组成材料的物理、化学性质,并且可以很好的取长补短,利用组成材料的优点,形成新的具有多种功能的纳米材料。近年来,随着人们生活水平的提高,人们对于所使用物品的组成材料也越来越关心,按照人们所需要的,新材料应该是是绿色的、环境友好的且对人体没有伤害的。但是如今很多纳米材料都有着各自的缺点,很难完全达到理想的标准,复合纳米粒子的出现恰好解决了这个问题,例如本文中选用了蛋白质作为主体,提高了材料的生物相容性和可降解性,还对其形成机理进行了探究,并且成功应用于生物医学领域。本文主要合成了两种复合纳米粒子,BSA-BPB复合纳米粒子和CTRA-MSNs复合纳米粒子。BSA-BPB复合纳米粒子是我们设计并合成的一种新型水溶性荧光蛋白纳米方块。加热BSA会形成一个类似于立方体的蛋白质框架,在加热过程中,利用BSA与BPB的结合能力,将BPB掺杂于蛋白质框架中,由于BPB本身具有聚集诱导发光(AIE)的特性,所以就形成了一种BSA-BPB纳米立方体(BBNCs)AIE-dots。此AIE-dots合成方法简单快速,合成原料低廉,并且蛋白质框架提供了一个非常好的生物相容性结构。在生物影像的实验中,它可以通过细胞的胞吞作用进入酵母和动物细胞,其荧光信号也可以穿透小鼠组织,非常适用于生物成像的领域。CRTA-MSNs复合纳米粒子,是我们设计的一个新型的基于介孔二氧化硅纳米粒子(MSNs)与α-糜蛋白酶A蛋白(CTRA)的超分子相互作用复合形成的纳米粒子,由于CTRA与其抑制剂苯基甲磺酰氟(PMSF)之间存在相互作用,并且引起CTRA发生蛋白构象的变化,CTRA-MSNs复合纳米粒子从而解组装。所以CTRA-MSNs复合纳米粒子可以装载药物,适用于药物控释系统。这种主客体控释系统可以在体外和细胞内平稳工作,并且这种类型的生物开关可以通过使用存在于自然界中的不同蛋白质-抑制剂,从而扩展到其它药物载体系统。
[Abstract]:Composite nanoparticles are nanoparticles made up of two or more molecules or nanoscale materials. They often have the physical and chemical properties of the constituent materials, and they can make good use of the advantages of the constituent materials. In recent years, with the improvement of people's living standards, people are more and more concerned about the composition of the materials used, according to what people need, the new materials should be green. But many nanomaterials today have their own shortcomings, and it is very difficult to fully meet the ideal standard. The appearance of composite nanoparticles just solves this problem. For example, in this paper, protein is chosen as the main body to improve the biocompatibility and biodegradability of the material, and the mechanism of its formation is also explored. In this paper, two kinds of composite nanoparticles, BSA-BPB composite nanoparticles and CTRA-MSNs composite nanoparticles. BSA-BPB composite nanoparticles are a new kind of water-soluble fluorescent eggs designed and synthesized. White nanometers. Heating BSA creates a protein frame that looks like a cube. In the heating process, using the binding ability of BSA and BPB, the BPB is doped into the protein frame. Because BPB has the characteristic of aggregation induced luminescence (AIEs), a kind of BSA-BPB nano-cube BBNCsO AIE-dots.This AIE-dots synthesis method is simple and rapid. The synthetic material is cheap, and the protein framework provides a very good biocompatibility structure. In bioimaging experiments, it can enter yeast and animal cells through the cellular endocytosis. Its fluorescence signals can also penetrate mouse tissues, and are well suited to the field of biological imaging. CRTA-MSNs composite nanoparticles, It is a new type of nanocrystalline particles based on mesoporous silica nanoparticles (MSNs) and 伪 -chymotrypsin A protein (CTRAA) supermolecular interaction. Because of the interaction between CTRA and its inhibitor Phenylmethanesulfonyl fluoride (PMSF), The protein conformation of CTRA is changed and CTRA-MSNs composite nanoparticles are unassembled. So the CTRA-MSNs composite nanoparticles can be loaded with drugs and can be used in drug controlled release system. This kind of host and guest controlled release system can work smoothly in vitro and in cells. And this type of biological switch can be extended to other drug carrier systems by using different protein-inhibitors that exist in nature.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;R318

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