SEBS表面图案化及其血液相容性的研究

发布时间：2018-02-20 01:59

  本文关键词： SEBS 糖基功能单体 表面图案化 血液相容性 水凝胶　出处：《烟台大学》2017年硕士论文　论文类型：学位论文

【摘要】：以D-(+)-葡萄糖酸-1,5-内酯和甲基丙烯酸-2-氨基乙基酯盐酸盐为原料,制备了含有不饱和双键的糖基功能单体-甲基丙烯酸-2-(N-葡萄糖酰胺)乙酯(GAMA),利用傅立叶红外光谱(FTIR)和氢核磁共振谱(1H NMR)对单体结构进行了表征。采用紫外引发表面接枝技术将GAMA接枝到聚(苯乙烯-b-(乙烯-co-丁二烯)-b-苯乙烯)(SEBS)膜表面,形成分子刷,对材料表面进行生物相容性改性,功能化的SEBS膜结构通过衰减全反射红外光谱(ATR-FTIR)和X-光电子能谱(XPS)进行分析,通过静态去离子水接触角、蛋白质吸附、血小板粘附以及溶血等实验,考察了改性后SEBS表面的亲水性和血液相容性。结果表明,与SEBS原膜相比,PGAMA分子刷改性的SEBS膜表面具有良好的亲水性、抗蛋白吸附、抗血小板粘附性能,并且有效的降低了溶血的发生。通过紫外引发表面接枝在SEBS表面接枝2-丙烯酰胺-2-甲基丙磺酸(AMPS)分子刷得到良好的亲水性表面,用于抵抗蛋白质吸附和血细胞粘附;然后,结合光掩模板在PAMPS分子刷表面制备图案化的PGAMA分子刷区域,从而构建了二元图案化的亲水性分子刷结构。随后利用糖与凝集素之间的作用,得到了尺寸可控、形貌均匀清晰的植物血球凝集素(PHA)阵列。利用PHA对红细胞的特异性识别作用,通过血细胞粘附实验发现,PHA阵列几乎不粘附血小板而能够高效捕捉红细胞(RBCs),并且捕获的红细胞基本保持其原有的完整性和功能。因此,实现了红细胞在材料表面的无损害捕获。通过紫外引发表面接枝技术方法在SEBS膜表面接枝第一层聚乙二醇(PEG)分子刷;然后采用紫外引发接枝聚合与光刻蚀技术相结合的方法,在第一层分子刷的基础上构建了结构层次清晰均匀,图案化区域边界明显的二元三维图案化水凝胶结构。利用ATR-FTIR和XPS对功能化的SEBS各层进行表面结构分析;利用偏光显微镜(POM)和扫描电子显微镜(SEM)等手段观察该体系的表面形貌;通过静态去离子水接触角、蛋白质吸附、血小板粘附以及红细胞粘附等实验,考察了该体系表面的亲水性和血液相容性。其中,第一层亲水层PEG分子刷,可以有效抵抗蛋白质和血细胞的粘附,第二层图案化水凝胶,由于表面亲水性和表面独特的拓扑结构的共同影响,既不吸附蛋白质也不粘附血细胞,因此,该体系能够很好的抵抗蛋白质的非特异性吸附和血细胞的粘附,进而改善了SEBS的血液相容性。
[Abstract]:D-Gluconoic acid-5-lactone and methacrylate -2-aminoethyl ester hydrochloride were used as raw materials, The glycosyl functional monomers of glycosyl groups with unsaturated double bonds were prepared. The structure of the monomers was characterized by Fourier transform infrared spectroscopy (FTIR) and hydrogen nuclear magnetic resonance spectroscopy (1H NMRs). GAMA was grafted onto the surface of poly (styrene-b-(ethylene-co- butadiene)-b-styrene) film by surface grafting. Molecular brushes were formed to modify the biocompatibility of the materials. The functionalized SEBS membrane structure was analyzed by attenuated total reflectance infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS), and the protein was adsorbed by static deionized water contact angle. The hydrophilicity and blood compatibility of modified SEBS surface were investigated by platelet adhesion and hemolysis experiments. The results showed that the surface of SEBS membrane modified by PGAMA molecular brush had good hydrophilicity and anti-protein adsorption. The anti-platelet adhesion property, and effectively reduced the occurrence of hemolysis. The surface of SEBS was grafted onto the surface of SEBS to obtain good hydrophilic surface. It is used to resist protein adsorption and blood cell adhesion; then, a patterned PGAMA molecular brush region is prepared on the surface of the PAMPS molecular brush by combining the photomask template. Thus, a binary patterned hydrophilic molecular brush structure was constructed. Then, by using the interaction between sugar and lectin, a controllable size and uniform morphology of plant hemagglutinin (PHA) array was obtained. The specific recognition effect of PHA on red blood cells was obtained. By blood cell adhesion test, it was found that PHA array could capture RBCsN efficiently and retain its original integrity and function. The red blood cells on the surface of the material were captured without damage. The first layer of polyethylene glycol (PEG) molecular brush was grafted on the surface of SEBS membrane by UV-induced surface grafting, and then the UV initiated graft polymerization was combined with photoetching. On the basis of the first layer molecular brush, a binary three-dimensional patterned hydrogel structure with clear and uniform structure and obvious boundary of patterned region was constructed. The surface structure of the functional SEBS layers was analyzed by ATR-FTIR and XPS. The surface morphology of the system was observed by means of polarizing microscope (POM) and scanning electron microscope (SEM). The surface morphology of the system was observed by static deionized water contact angle, protein adsorption, platelet adhesion and erythrocyte adhesion. The surface hydrophilicity and blood compatibility of the system were investigated. The first hydrophilic layer PEG molecular brush could effectively resist the adhesion of protein and blood cells, and the second layer was patterned hydrogel. Because of the influence of surface hydrophilicity and surface unique topological structure, neither protein nor blood cells are adsorbed. Therefore, the system can resist the non-specific adsorption of proteins and the adhesion of blood cells. Thus, the blood compatibility of SEBS was improved.
【学位授予单位】：烟台大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB306

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本文编号：1518495