基于不同保护剂制备的银纳米颗粒的抗菌性能研究

发布时间：2018-02-24 11:06

本文关键词： 纳米银抗菌材料抗菌效果机理　出处：《安徽农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：随着生活水平的提高,人们对生活坏境的关注也越来越重视,因此对抗菌材料的研究也越来越受到重视。纳米银作为一种重要的纳米抗菌剂,其广谱、高效的抗菌性能,获得了研究人员极大的关注。该实验分别制备了两种不同的纳米银颗粒,并对其抗菌效果、抗菌机理进行了初步的研究。本论文分别以聚乙烯吡咯烷酮(PVP)和葡聚糖(Dextran)为保护剂制备了两种不同的银纳米颗粒,并且利用紫外-可见分光光度计(UV-vis)、动态光散射(DLS)等方法对制备的材料进行了性能表征。该实验采用生长曲线、涂布平板、WST-8试剂盒、可溶性总糖的测定进行研究,对这两种材料的抑菌效果进行比较分析,同时对其抑菌机理进行了初步探讨,主要研究成果如下：1.制备了分散性良好、性能稳定的PVP-Ag及Dextran-Ag纳米颗粒。2.以PVP为保护剂制备的银纳米颗粒对革兰氏阴性菌(大肠杆菌)的影响。201μg/mL的银纳米颗粒能够完全抑制107cfu/mL的大肠杆菌的生长。以PVP为保护剂制备的银纳米颗粒对革兰氏阳性菌(金黄色葡萄球菌)的影响。151μg/mL的银纳米材料能够完全抑制107cfu/mL的金黄色葡萄球菌的生长。3.以葡聚糖为保护剂制备的银纳米颗粒对革兰氏阴性菌(大肠杆菌)的影响。151μg/mL的银纳米颗粒能够完全抑制107cfu/mL的大肠杆菌的生长。以葡聚糖为保护剂制备的银纳米颗粒对革兰氏阳性菌(金黄色葡萄球菌)的影响,20μg/mL的银纳米材料能够完全抑制107 cfu/mL的金黄色葡萄球菌的生长。
[Abstract]:With the improvement of living standard, people pay more and more attention to the bad environment of life, so more and more attention is paid to the research of antimicrobial materials. As an important nanometer antimicrobial agent, silver nanoparticles have wide spectrum and high antibacterial properties. Two different kinds of silver nanoparticles were prepared and their antimicrobial effects were studied. In this paper, two different silver nanoparticles were prepared by using PVP and Dextranan as protective agents, respectively, in the presence of polyvinylpyrrolidone (PVP) and dextran (Dextranan) as protective agents. The properties of the prepared materials were characterized by UV-vis spectrophotometer and dynamic light scattering (DLSs). The growth curve, the coated plate WST-8 kit and the determination of total soluble sugar were studied. The bacteriostatic effect of the two materials was compared and analyzed, and the antibacterial mechanism was preliminarily discussed. The main research results were as follows: 1.The dispersity of the two materials was good. Stable PVP-Ag and Dextran-Ag nanoparticles. 2. The effect of silver nanoparticles prepared with PVP as protectant on gram-negative bacteria (E. coli). 201 渭 g / mL silver nanoparticles could completely inhibit the growth of 107 cfur / mL Escherichia coli. PVP was used to protect the growth of Escherichia coli. Effect of silver nanoparticles prepared by preservatives on Gram-positive bacteria (Staphylococcus aureus). 151 渭 g / mL silver nanomaterials could completely inhibit the growth of 107cfu / mL Staphylococcus aureus. 3. Silver nanoparticles prepared with dextran as protectant. The effect of granulocytes on Gram-negative bacteria (Escherichia coli). 151 渭 g / mL silver nanoparticles could completely inhibit the growth of 107 cfur / mL Escherichia coli. Silver nanoparticles prepared with dextran as protective agent against Gram-positive bacteria (Grape aureus). The silver nanomaterials of 20 渭 g / mL could completely inhibit the growth of Staphylococcus aureus at 107 cfu/mL.
【学位授予单位】：安徽农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O614.122;TB34

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