纳米金颗粒与牛血清蛋白作用机制的研究

发布时间：2018-03-01 16:34

本文关键词： 金纳米颗粒牛血清蛋白(BSA) 单分散结合结合位点榫hY嵌合　出处：《内蒙古农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：随着纳米技术的发展,·纳米颗粒由于其优质的物理和化学性质,使其在科学研究及工业领域得到了广泛的关注。然而,当一些功能化的纳米颗粒应用于生物体内时,生物体内的蛋白迅速的吸附在纳米颗粒的表面,形成纳米颗粒-蛋白质冠的结构,导致了纳米颗粒预先设计功能的改变。因此,探索蛋白质与纳米颗粒的作用机制对功能性纳米颗粒在生物体内功能的发挥是至关重要的。本文以牛血清蛋白为例,通过牛血清蛋白(BSA)与纳米颗粒的物理吸附、蛋白酶酶解、结合聚丙烯酰胺凝胶电泳(PAGE)和基质辅助激光解吸／电离质谱-飞行时间质谱(MALDI-TOF-MS)的鉴定,我们发现,BSA中有107个氨基酸、分子量大小约为12.8kDa的肽链为BSA与AuNP的结合位点。为了进一步解释为什么会有如此长的肽段被金纳米颗粒的表面所保护,以至于不受蛋白酶酶解,我们提出了一种新颖的机械榫眼和榫舌嵌合的结合模式,其中充当榫舌的结合肽插入到疏水槽金纳米粒子的表面上的榫眼中。我们选取分子量小于BSA的牛血红蛋白(Hgb),通过化学连接的方式并结合分子筛以及动态光散色仪两种方式来认证该模型的可能性,另外小分子布洛芬、SDS与BSA竞争结合纳米金颗粒的实验来进步证明该模型的正确性。
[Abstract]:With the development of nanotechnology, nanoparticles have attracted wide attention in the field of scientific research and industry because of their excellent physical and chemical properties. However, when some functional nanoparticles are used in organisms, Proteins are rapidly adsorbed on the surface of nanoparticles, forming the structure of nanocrystalline protein crowns, resulting in changes in the pre-designed function of nanoparticles. It is very important to explore the mechanism of action between protein and nanoparticles for the function of functional nanoparticles in vivo. In this paper, bovine serum protein (BSA) is used as an example to study the physical adsorption and enzymatic hydrolysis of bovine serum protein (BSA) with nanoparticles. Combined with the identification of polyacrylamide gel electrophoresis (page) and matrix assisted laser desorption / ionization mass spectrometry (MALDI-TOF-MS), we found that there are 107 amino acids in BSA. A peptide chain with a molecular weight of about 12.8kDa is the binding site between BSA and AuNP. To further explain why such a long peptide segment is protected from proteolysis by the surface of gold nanoparticles, We propose a novel mechanical mortise and tongue-tongue-inlay mode. The binding peptide, which acts as a tenon, is inserted into the tenon hole on the surface of gold nanoparticles. We select bovine hemoglobin (HGB) with molecular weight less than BSA, and combine it with molecular sieve and dynamic photochromograph by chemical bonding. There are two ways to authenticate the possibility of the model, In addition, small molecule ibuprofen SDS competes with BSA in the experiment of gold nanoparticles to prove the correctness of the model.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O614.123

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