基于多功能纳米探针的细胞实时监测

发布时间：2018-03-09 17:34

  本文选题：荧光探针　切入点：温敏高聚物　出处：《华东师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：纳米材料具有颗粒小、可修饰性强、特异性识别等特性,因此其复合材料在化学分析、生物传感和医疗等领域扮演着重要角色。在医学上,纳米材料的应用常侧重于生物传感和药物运输。癌症的早期诊断与有效治疗仍是当前医疗上的一大瓶颈,本文设计合成了一种可用于各种比率化的温度相关检测以及癌症诊疗应用的温敏核壳复合荧光纳米材料(CD-MSN@UP38),并将其应用在细胞内温度检测和活体癌症诊疗中。该材料作为温度传感器时,装载极性敏感染料BBD并结合荧光寿命显微成像技术,能做到细胞内温度的比率化准确检测以及单个细胞内温度分布,具有极高的时空分辨率和温度分辨(0.2℃)。更有意思的是,CD-MSN@UP38粒子可以进一步作为温控药物传递系统应用于癌症治疗,由癌细胞本身的热效应触发释药。该智能载药物治疗系统在活体实验中显示了高效疗和低副作用。另一方面,在生物分析领域中,由于人脐带间充质干细胞(hMSCs)可以分化为神经干细胞(NSCs),在中枢神经系统紊乱和细胞置换疗法中有巨大的潜在应用。端粒酶(Telomerase,TE)可以添加端粒(TTAGGG)n重复到人的染色体末端,与干细胞寿命息息相关。然而,其活性在维持hMSCs细胞增殖过程中的生物学特性以及分化潜力变化中扮演的角色仍是未知数。本文开拓了一种新型诱导细胞自发合成荧光cDNA银纳米簇(cDNA-AgNCs),长期实时成像hMSCs细胞增殖和分化过程中TE活性的变化。该生物原位合成的以DNA为模板的荧光AgNCs尺寸小、光稳定性好且生物相容性优异,突破了外源合成的纳米复合材料易被免疫排斥而不能长期在生物内传感的瓶颈。
[Abstract]:Nanomaterials play an important role in the fields of chemical analysis, biosensor and medical treatment because of their small particle size, strong modifiability, specificity recognition and so on. The application of nanomaterials is often focused on biosensors and drug transport. Early diagnosis and effective treatment of cancer remains a major medical bottleneck. In this paper, we have designed and synthesized a thermo-sensitive core-shell composite fluorescent nanomaterial, CD-MSN @ UP38, which can be used for various ratios of temperature correlation detection and cancer diagnosis and treatment. The material has been applied to intracellular temperature detection and in vivo cancer diagnosis and treatment. When the material acts as a temperature sensor, Loaded with polarity sensitive dye BBD and fluorescence lifetime microimaging technology, the ratio of intracellular temperature and the temperature distribution in a single cell can be measured accurately. It has a very high space-time resolution and a temperature resolution of 0.2 鈩，

本文编号：1589548