基于低维纳米材料的可穿戴电化学生物传感器电极研究

发布时间：2018-03-11 04:28

本文选题：电化学　切入点：生物传感器　出处：《深圳大学》2017年硕士论文　论文类型：学位论文

【摘要】：随着电化学生物传感器的不断发展,出现了众多性能优异,测试精准的电化学生物传感器,并被运用于医疗、安全检测、食品安全和环境保护等多个领域。由于生物传感器具有检测过程简单,准确性高,灵敏度高等优点,成为各种检测应用的研究重点。但随着电化学生物传感器的不断普及,其研究更多是考虑到用户的需求,传感器设备逐渐趋于微型化、便携化。因此,近年来出现了印刷电极、酶膜反应器等新型传感器。他们除了具有检测的高效性和稳定性外,还具有方便用户使用与测量的优势。本文通过制备出柔性葡萄糖和乳酸传感器,并制备出性能优异的酶膜传感器,实现传感器的高效化、便携化和微型化等目的。首先,通过在SBS薄膜中掺入PEDOT:PSS水溶液以及MWNT-OH制备出导电高分子薄膜,并作为一种传感器电极使用,使用壳聚糖与戊二醛的交联来固定氧化酶,制备出电化学性能良好的柔性葡萄糖生物传感器和柔性乳酸生物传感器。制备的柔性葡萄糖生物传感器,其检测线性范围为0~6 m M,线性回归方程为I=2.21*c+1.4455,R~2=0.996,检测灵敏度为2.21μA/m M,检出限为12μM(S/N=3)。制备的柔性乳酸生物传感器,其检测线性范围为0.2~1.6 m M,线性回归方程为I=7.00*c+0.5796,R~2=0.9935,检测灵敏度为7.00μA/m M,检出限为7.87μM(S/N=3)。这两种传感器均具有良好的稳定性和抗干扰性能。其次,通过改进后的Hummers法成功制备出氧化石墨烯,并通过溶剂热法制备出G-Pt NPs,作为电化学敏感材料制备出乳酸生物传感器,具有良好的检测稳定性和抗干扰性能,其检测线性范围为0~1.2 m M,线性回归方程为I=2.08*c+0.1623,R~2=0.990,检测灵敏度为2.08μA/m M,检出限为12.5μM(S/N=3)。同时,所制备出的柔性乳酸生物传感器,具有良好的检测稳定性和重现性,检测灵敏度为2.08μA/m M,通过对人体汗液进行实时检测,发现该柔性乳酸传感器可以对人体汗液进行检测。最后,通过在PVB薄膜中加入MWNT-OH制备导电薄膜基底,修饰上氧化酶,制备出性能优异的葡萄糖和乳酸生物传感器。其中,使用1%PVB制备出的葡萄糖传感器,其检测线性范围为0~3 m M,线性回归方程为I=0.03*c+0.0305,R~2=0.993,检测灵敏度为0.03μA/m M,检出限为49.1μM(S/N=3)。加入TTF后,其检测线性范围增加到为0~32 m M,线性回归方程为I=0.26*c+0.4899,R~2=0.990,检测灵敏度为0.26μA/m M,检出限为62.8μM(S/N=3),线性检测浓度范围大大提高。另外,使用1%PVB制备出的乳酸传感器,其检测线性范围为0.5~1.5 m M,线性回归方程为I=0.20*c+0.0967,R~2=0.992,检测灵敏度为0.20μA/m M,检出限为18.8μM(S/N=3)。加入TTF后,使用0.1%GA固定LOx制备出乳酸传感器,其检测线性范围为2~5 m M,线性回归方程为I=1.51*c+1.4956,R~2=0.991,检测灵敏度为1.51μA/m M,检出限为2.98μM(S/N=3),线性检测浓度范围有所提高。
[Abstract]:With the development of electrochemical biosensors, many electrochemical biosensors with excellent performance and accurate testing have been used in medical and safety detection. Food safety, environmental protection and other fields. Because biosensor has the advantages of simple detection process, high accuracy, high sensitivity and so on, it has become the research focus of various detection applications. However, with the continuous popularization of electrochemical biosensor, In recent years, new types of sensors, such as printed electrodes and enzyme membrane reactors, have emerged. In addition to the high efficiency and stability of detection, the sensor devices tend to be miniaturized and portable. In this paper, the flexible glucose and lactic acid sensors and the enzyme membrane sensors with excellent performance are prepared to achieve the high efficiency, portability and miniaturization of the sensors. Conductive polymer films were prepared by adding PEDOT:PSS aqueous solution and MWNT-OH into SBS thin films and used as a sensor electrode to immobilize oxidase by cross-linking chitosan and glutaraldehyde. A flexible glucose biosensor and a flexible lactate biosensor with good electrochemical performance were prepared. The linear range of detection is 0 ~ 6 mm, and the linear regression equation is I ~ (2.21) C ~ (1.4455A) R ~ (2 +) (0.996). The detection sensitivity is 2.21 渭 A / m ~ (m) and the detection limit is 12 渭 m ~ (-1) S / N ~ (3). A flexible lactic acid biosensor is prepared. The linear range of the two sensors is 0.2mM, the linear regression equation is 0.5796C, the sensitivity is 7.00 渭 A / m and the detection limit is 7.87 渭 M / N ~ (3 +). The two kinds of sensors have good stability and anti-interference performance. Secondly, graphene oxide has been successfully prepared by the improved Hummers method, and the detection limit is 7.87 渭 m 路m ~ (-1) 路m ~ (-1), and the detection limit is 7.87 渭 m ~ (-1) 路m ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). G-Pt NPswas prepared by solvothermal method and lactic acid biosensor was prepared as an electrochemical sensitive material. It has good stability and anti-interference performance. The linear range of detection is 0 ~ 1.2 mm, and the linear regression equation is I _ (2.08) ~ (0.1623) N ~ (2 +) O ~ (2 +), the sensitivity is 2.08 渭 A / m ~ (m), the detection limit is 12.5 渭 m ~ (-1) S / N ~ (3 +). At the same time, the prepared flexible lactate biosensor has good detection stability and reproducibility. The sensitivity of detection is 2.08 渭 A / m M. by real-time detection of human sweat, it is found that the flexible lactate sensor can detect the sweat of human body. Finally, the substrate of conductive film is prepared by adding MWNT-OH to the PVB film, and the oxidase is modified. Glucose and lactic acid biosensors with excellent performance were prepared. The linear range of glucose biosensor prepared by 1PVB was 0 ~ 3 mm, and the linear regression equation was I _ (0.03) C _ (0.0305) ~ R ~ (2 +) 0.993.The detection sensitivity was 0.03 渭 am 路m ~ (-1) and the detection limit was 49.1 渭 m 路s ~ (-1) N ~ (3 +). After adding TTF, the linear regression equation was obtained. The linear range of detection was increased to 0 ~ 32 mm, and the linear regression equation was I _ (0.26) C _ (0.4899) R ~ (2 +) 0.90. The detection sensitivity was 0.26 渭 A / m 路m ~ (-1) and the detection limit was 62.8 渭 m ~ (-1) m ~ (-1) 路m ~ (-1). The linear detection concentration range was greatly increased. In addition, the lactic acid sensor was prepared by using 1PVB. The linear range of detection is 0.5 ~ (1.5) mm, the linear regression equation is I _ (0.20) C 0.0967H ~ (2 +) ~ (2 +) 0.992.The detection sensitivity is 0.20 渭 A / m 路m ~ (-1) and the detection limit is 18.8 渭 m ~ (-1) m ~ (-1). After adding TTF, a lactic acid sensor is prepared with 0.1 GA fixed LOx. The linear range of detection was 2 渭 m / m, the linear regression equation was 1.4956 渭 m / m, the sensitivity was 1.51 渭 A / m / m and the detection limit was 2.98 渭 M / N ~ (-1) N ~ (3 +). The linear detection concentration range was improved.
【学位授予单位】：深圳大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;TP212

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