B. cepacia TZ-1合成磁性纳米颗粒的类酶性质及其对染料脱色的研究

发布时间：2018-03-22 05:26

本文选题：洋葱伯克霍尔德氏菌　切入点：生物矿化　出处：《东北林业大学》2015年硕士论文　论文类型：学位论文

【摘要】：从山东省聊城市东昌湖水域取湖水样品,通过富集培养、分离纯化的方法筛选出一株产磁性纳米颗粒的菌株,经生理生化测定及16S rDNA测序分析结果,鉴定为洋葱伯克霍尔德氏菌(Burkholderia cepacia),将其命名为B. cepacia TZ-11。B. cepacia TZ-1为兼性厌氧菌,在氧分压低于1%条件下才能合成磁性纳米颗粒,本研究独创性采用微氧密封、120 r/min摇床培养的方法来培养B. cepacia TZ-1。B. cepacia TZ-1为杆菌,菌体大小约1-2μm×0.5-1 μm,有鞭毛,革兰氏阴性。在平板上菌落直径约为3-5mm、呈圆形、表面光滑、边缘不规则、菌落湿润呈黄绿色、半透明、有黏性。透射电镜下可观察到菌体细胞壁周围有大小约60 nm致密晶体颗粒围成一圈、菌体内部含有3-5颗180 nm的致密晶体颗粒。采用超声破碎方法提取纯化磁性纳米颗粒,透射电镜、扫描电镜对其进行元素分析,结果一致,主要元素为Fe、P、O,X射线衍射分析磁性纳米颗粒成分为Fe3(PO4)2·8H2O、Fe3O4,磁性纳米颗粒为单斜晶体。磁性纳米颗粒在H202存在条件下具有过氧化物酶类酶活性,与商品HRP进行比较,磁性纳米颗粒对TMB的亲和力稍强,且对于H202浓度、pH、温度有极强的耐受性。磁性纳米颗粒在H202存在条件下可以催化染料脱色,本研究选择偶氮类染料甲基橙、蒽醌类染料酸性媒介红、芳甲烷类染料固绿FCF、靛族染料靛红作为研究对象,磁性纳米颗粒对上述4种染料均具有较好的降解脱色效果,且磁性纳米颗粒对染料脱色过程中,有极强的H202浓度、pH、温度耐受性。
[Abstract]:A strain producing magnetic nanoparticles was isolated from lake water samples from Dongchang Lake, Liaocheng City, Shandong Province. The results of physiological and biochemical analysis and 16s rDNA sequencing were analyzed. It was identified as Burkholderia cepacia of Onion and named B. cepacia TZ-11.B. cepacia TZ-1 as facultative anaerobes. The magnetic nanoparticles could be synthesized only when the oxygen partial pressure was lower than 1%. In this study, the microoxygen seal 120 r/min shaker culture method was used to culture B. cepacia TZ-1.B. cepacia TZ-1. The bacteria were about 1-2 渭 m 脳 0.5-1 渭 m in size, flagellum and Gram-negative. The diameter of colony on the plate was about 3 ~ 5 mm, the surface was round, the surface was smooth, and the edge was irregular. The colony was yellowish green, translucent and sticky. Under transmission electron microscope, it was observed that there were about 60 nm dense crystal particles around the cell wall in a circle. There were 3 to 5 dense crystal particles of 180 nm in the cell. The magnetic nanoparticles were extracted and purified by ultrasonic fragmentation. The results were consistent with the results of elemental analysis by transmission electron microscope and scanning electron microscope. The main element is Fe3(PO4)2 8 H 2O O 3 O 4, and the magnetic nanoparticles are monoclinic crystals. The magnetic nanoparticles have peroxidase activity in the presence of H202, and are compared with commercial HRP. Magnetic nanoparticles have stronger affinity to TMB, and have strong tolerance to H202 pH and temperature. Magnetic nanoparticles can catalyze the decolorization of dyes in the presence of H202. In this study, azo dyes methyl orange was selected. Anthraquinone dyes, acid mordant red, aromatic methane dyes, solid green FCFs and indigo dye indirubin were studied. The magnetic nanoparticles had good degradation and decolorization effect on the above four dyes, and the magnetic nanoparticles decolorized the dyes. It has strong pH value of H 202 and temperature tolerance.
【学位授予单位】：东北林业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1

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