三种不同银纳米粒子SERS基底比较研究

发布时间：2018-03-23 08:43

本文选题：表面增强拉曼散射　切入点：毛细管　出处：《大连理工大学》2015年硕士论文　论文类型：学位论文

【摘要】：表面增强拉曼光谱(Surface-Enhanced Raman Spectra,简称SERSp)技术是一种新兴的分析检测技术,由于其对样品分析时,具有灵敏度高、检测时间短以及样品所需量小等优点,在最近几年里,SERSp技术已在众多的领域如：生物医学,药物学,化学等都得到了广泛的应用,同时表面增强拉曼散射(Surface-Enhanced Raman Scattering,简称SERS)基底的制备也已成为该领域的研究热点。本文不仅介绍了拉曼光谱相关的理论知识,而且重点对基于银纳米粒子的三种SERS基底进行了研究比较。本文的主要内容如下：首先,本文从拉曼散射的基本原理、拉曼光谱的基本特征以及拉曼光谱在生物医学及药物学中的应用与前景这几方面对拉曼光谱进行了概述。其次,对表面增强拉曼光谱进一步论述,介绍了表面增强拉曼光谱的发现与发展,同时重点介绍了表面增强拉曼散射的两种增强机理：基于电磁场效应的增强机理和基于化学效应的增强机理。最后,重点对三种以银纳米粒子(AgNPs)的SERS效应为基质的拉曼活性基底：毛细管-AgNPs基底,二氧化钛-AgNPs基底和滤纸-AgNPs基底进行了比较研究。分别用这三种基底对罗丹明6G(R6G)分子进行拉曼光谱数据的采集及分析,从而找出这三种SERS基底相应的最佳制备条件。最后用这三种最佳条件下制备的SERS基底对同一个健康人血清进行拉曼光谱检测,并对结果进行分析比较。初步结果：这三种基于AgNPs的SERS基底都是可靠的和实用的；二氧化钛-AgNPs基底灵敏度相对较高,但制备过程较复杂；滤纸-AgNPs基底灵敏度其次；毛细管-AgNPs基底及滤纸AgNPs基底的制备均较为简单。因此,从实用的角度考虑,滤纸-AgNPs基底比较适合血清的表面增强拉曼光谱的检测与分析。
[Abstract]:Surface-enhanced Raman Spectra (SERSpS) technology is a new analytical and detection technique, which has the advantages of high sensitivity, short detection time and small amount of sample. In recent years, SERSp technology has been widely used in many fields such as biomedicine, pharmacology, chemistry and so on. At the same time, the preparation of surface-enhanced Raman scattering (SERS-based) substrates has become a hotspot in this field. Three kinds of SERS substrates based on silver nanoparticles are studied and compared. The main contents of this paper are as follows: firstly, the basic principle of Raman scattering is introduced. The basic characteristics of Raman spectrum, the application and prospect of Raman spectrum in biomedicine and pharmacology are summarized. Secondly, surface enhanced Raman spectroscopy is further discussed. In this paper, the discovery and development of surface-enhanced Raman spectroscopy are introduced, and two enhancement mechanisms of surface-enhanced Raman scattering are emphasized: the enhancement mechanism based on electromagnetic field effect and the enhancement mechanism based on chemical effect. Three kinds of Raman active substrates based on the SERS effect of silver nanoparticles were focused on: capillary-AgNPs substrates. A comparative study of titanium dioxide-AgNPs substrate and filter paper-AgNPs substrate was carried out. The Raman spectra data of Rhodamine 6GN R6G) molecules were collected and analyzed by using these three substrates. The optimal preparation conditions of the three SERS substrates were found out. Finally, the Raman spectra of the same healthy serum were detected by using the SERS substrates prepared under these three optimal conditions. The three kinds of SERS substrate based on AgNPs are all reliable and practical, the sensitivity of titania-AgNPs substrate is relatively high, but the preparation process is more complicated, the sensitivity of filter paper-AgNPs substrate is second; The preparation of capillary-AgNPs substrate and filter paper AgNPs substrate is relatively simple. Therefore, the filter paper-AgNPs substrate is more suitable for the detection and analysis of serum surface enhanced Raman spectroscopy from a practical point of view.
【学位授予单位】：大连理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1

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