功能化介孔硅材料的合成及其对富含裸露组氨酸蛋白质的选择性吸附

发布时间：2018-03-23 21:36

本文选题：自由基聚合　切入点：介孔硅　出处：《色谱》2017年03期

【摘要】：通过自由基聚合反应合成了具有高比表面积的介孔硅(PPOSS),并对其表面进行修饰和铜离子(Cu~(2+))的固定,最终得到功能化介孔硅材料(PPOSS-IDA-Cu~(2+)),然后利用PPOSS-IDA-Cu~(2+)中的Cu~(2+)与蛋白质裸露组氨酸的螯合作用,选择性吸附富含裸露组氨酸的蛋白质。对牛血红蛋白、牛血清白蛋白、肌红蛋白和溶菌酶的吸附结果表明,所合成的PPOSS-IDA-Cu~(2+)材料对富含组氨酸的牛血红蛋白有较好的吸附选择性和较大的吸附容量(3 150mg/g),并且有望与其他材料联合使用,以检测到更多相对丰度较低的蛋白质,丰富人类蛋白质组学信息,为疾病的临床诊断及病理研究提供帮助。
[Abstract]:A mesoporous silica PPOSS with high specific surface area was synthesized by free radical polymerization, and its surface was modified and fixed by copper ion. Finally, the functionalized mesoporous silicon material PPOSS-IDA-CuO2 was obtained, and then the chelation of Cu~(2 with protein exposed histidine was used to selectively adsorb the protein rich in naked histidine, and to treat bovine hemoglobin, bovine serum albumin, bovine hemoglobin, bovine serum albumin, bovine hemoglobin, bovine serum albumin, bovine hemoglobin, bovine serum albumin, bovine hemoglobin, bovine serum albumin (BSA). The adsorption results of myoglobin and lysozyme showed that the synthesized PPOSS-IDA-Cu~(2) had good adsorption selectivity and adsorption capacity for histidine rich bovine hemoglobin, and was expected to be used in combination with other materials. In order to detect more proteins with low relative abundance and enrich human proteomics information, it is helpful for clinical diagnosis and pathological study of diseases.
【作者单位】：兰州大学化学化工学院;兰州大学中卫高新技术研究院;
【基金】：国家自然科学基金项目(21105039,21375052)~~
【分类号】：TB383.4;O647.3;O613.72

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