共轴静电纺丝制备新型生物功能材料

发布时间：2018-03-24 00:19

本文选题：静电纺丝　切入点：核壳结构　出处：《浙江理工大学》2017年硕士论文

【摘要】：1.用共轴静电纺丝的方法将天然高分子肝素和纤维素溶解在1-乙基-3-甲基咪唑醋酸盐(1-ethyl-3-methylimidazolium acetate,[EMIM][Ac])离子液体作为壳溶液,同时将四氧化三铁纳米颗粒(5 nm)溶解在[EMIM][Ac]离子液体作为核溶液,高压纺丝后得到具有核壳结构的纤维。并用SEM,TEM对其表面形貌和内部结构进行了表征,用EDX元素分析确定了复合纤维材料元素的组成,用XRD对纤维素及四氧化三铁纳米颗粒的晶型物相进行了分析,用TGA热重分析的方法确定了核壳纤维中四氧化三铁的含量,LC-MS液相色谱与质谱联用技术确定了肝素的存在,同时也对肝素、纤维素和四氧化三铁核壳纤维进行了Xa和IIa抗凝血活性分析,结果表明壳结构里包含肝素的核壳纤维具有很好的抗凝血活性。2.用共轴静电纺丝的方法将合成高分子聚己内酯(polycaprolactone,PCL)溶于2,2,2-三氟乙醇溶剂中作为纺丝壳溶液,同时分别将40%,60%,80%(w/v)合成弹性高分子聚合物聚癸二酸丙三醇酯(poly-glycerol sebacate,PGS)溶于2,2,2-三氟乙醇溶剂中作为核溶液进行纺丝,为了突出具有核壳结构PCL/PGS支架材料形貌、机械强度等方面的特点,将PCL和PGS溶于2,2,2-三氟乙醇溶剂的混合溶液进行单泵纺丝作为对照。另一方面,对于具有核壳结构的PCL/PGS纤维,由于PCL是疏水性材料,缺乏血管细胞生长的附着位点,同时作为人工血管材料要有一定的抗凝血性,我们通过化学的方法将肝素嫁接了在PCL/PGS支架表面。我们对材料的化学性能、机械性能以及生物性能进行了表征,PCL/PGS核壳材料具有可控制的降解性能以及可调谐的机械性能。降解缓慢的PCL外壳主要作为机械支撑和结构框架,降解快速的PGS材料则增加了材料的弹性性能,随着PGS/PCL比例的变化,得到材料的杨氏模量变化范围是5.6M-15.7M,极限拉伸应力变化范围是2.0M-2.9M,伸长率的变化范围是291.4%-906.8%,该材料在组织工程应用方面提供了可能。
[Abstract]:1. Natural macromolecule heparin and cellulose were dissolved in 1-ethyl-3-methylimidazolium acetate1 ethyl-3-methylimidazolium acetate, [EMIM] [ac] ionic liquid as shell solution by coaxial electrospinning, and iron trioxide nanoparticles (5 nm) were dissolved in [EMIM] [ac] ionic liquid as nuclear solution. The fibers with core-shell structure were obtained by high pressure spinning. The surface morphology and internal structure of the fibers were characterized by EDX, and the composition of the composite fibers was determined by EDX elemental analysis. The crystalline phase of cellulose and iron trioxide nanoparticles was analyzed by XRD. The content of iron trioxide in core-shell fiber was determined by TGA thermogravimetric analysis. The presence of heparin was determined by LC-MS liquid chromatography and mass spectrometry. The anticoagulant activities of heparin, cellulose and ferric oxide core-shell fibers were also analyzed by Xa and IIa. The results show that the core-shell fibers containing heparin have good anticoagulant activity. The synthetic polycaprolactone polycaprolactone PCL (PCL) is dissolved as a spinning shell solution in 2C 2N 2fluoroethanol solvent by coaxial electrostatic spinning. At the same time, the elastic polymer poly-glycerol sebacate (PGSs), which was synthesized from 40 / 60 / 80 / w / v / w / / /,, respectively, was spun as a nuclear solution in the solvent of 2o 2o 2 fluoroethanol, in order to highlight the characteristics of the PCL/PGS scaffold with core-shell structure, such as morphology, mechanical strength, and so on. In contrast, PCL and PGS were spun by single pump in the mixed solution of 2H 2H 2F ethanol solvent. On the other hand, for PCL/PGS fibers with core-shell structure, because PCL was hydrophobic, there was no adhesion site for the growth of vascular cells. At the same time, as an artificial blood vessel material to have a certain degree of anticoagulant, we chemically grafted heparin on the surface of the PCL/PGS scaffold. The mechanical and biological properties of PCL / PGS core-shell materials are characterized by their controllable degradation and tunable mechanical properties. The slow-degrading PCL shells are mainly used as mechanical support and structural framework. The fast degradable PGS material increased the elastic properties of the material, with the change of PGS/PCL ratio. The Young's modulus is 5.6M-15.7m, the ultimate tensile stress is 2.0M-2.9M, and the elongation is 291.4-906.8m, which provides the possibility for the application of the material in tissue engineering.
【学位授予单位】：浙江理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB34

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