Cu-Ti-O纳米管阵列的抗菌性能及其对内皮细胞和成骨细胞功能的影响

发布时间：2018-05-06 07:55

本文选题：阳极氧化 + 纳米管　；参考：《太原理工大学》2017年硕士论文

【摘要】：钛及钛合金具有优良的生物相容性、良好的机械性能、耐腐蚀性及核磁共振成像的相容性等优点,已被用作植入材料广泛应用于整形外科领域,但是植入后的感染和松动等并发症长期困扰医患双方。与传统的微观和宏观形貌相比,纳米级结构对调节细胞功能更加有效,其中通过阳极氧化在纯钛(Ti)表面构建的二氧化钛(TiO_2)纳米管阵列结构引起了人们的广泛关注,因为适当尺寸的TiO_2纳米管不仅可促进成骨细胞的成骨分化,也可促进内皮细胞的成血管能力。然而TiO_2纳米管阵列抗菌能力差,无法有效对抗植入体感染。本研究首先在纯Ti片表面利用磁控溅射技术沉积了不同Cu含量的TiCu涂层,然后通过阳极氧化成功地将Cu掺杂至TiO_2纳米管阵列中,并研究了Cu掺杂TiO_2纳米管阵列的长效抗菌性能、细胞相容性及其对内皮细胞和成骨细胞功能的影响。结果表明:(1)利用磁控溅射技术在纯Ti表面沉积不同Cu含量的TiCu复合涂层,Cu的掺杂量可在0 at.%和20.04at.%之间调控;(2)当TiCu涂层中Cu的原子百分比不大于15.14%时,通过阳极氧化可以构建出高度有序的Cu-Ti-O纳米管阵列;当TiCu涂层中Cu的原子百分比为20.04%时,制备出的为纳米孔状结构;(3)Cu-Ti-O纳米管阵列持续释放的铜离子(Cu~(2+))对革兰氏阳性金黄色葡萄球菌表现出良好的长效抗菌能力;(4)所有纳米管阵列表面对内皮细胞的粘附、活性影响不大,且适量的Cu掺杂能够促进内皮细胞的铺展,和TiO_2纳米管阵列(对照组)相比,Cu-Ti-O纳米管阵列表面及其离子提取物均能够上调内皮细胞一氧化氮(NO)的合成和血管内皮生长因子(VEGF)的分泌,而且Cu-Ti-O纳米管阵列的离子提取物培养内皮细胞的体外成血管能力也有所提高;(5)Cu-Ti-O纳米管阵列对成骨细胞的碱性磷酸酶(ALP)活性无显著影响;诱导分化早期,由于Cu~(2+)释放量较大,Cu-Ti-O纳米管阵列能够促进Ⅰ型胶原蛋白的沉积,同时对细胞外基质矿化表现出一定的抑制作用,但随着诱导培养时间的延长,这种现象变得越来越不明显甚至消失。因此良好的抗菌性能、骨生成和血管生成能力使得Cu-Ti-O纳米管阵列成为一种比较有前景的钛植入体涂层。
[Abstract]:Titanium and titanium alloys have been widely used in plastic surgery due to their excellent biocompatibility, good mechanical properties, corrosion resistance and compatibility of nuclear magnetic resonance imaging. But complications such as infection and loosening after implantation have long plagued both doctors and patients. Compared with traditional microcosmic and macroscopic morphology, nanostructures are more effective in regulating cell function. Among them, TiO2 / TiO2) nanotube array structure constructed by anodic oxidation on the surface of pure titanium (Ti) has attracted much attention. The appropriate size of TiO_2 nanotubes can not only promote the osteogenic differentiation of osteoblasts, but also promote the vascular formation ability of endothelial cells. However, TiO_2 nanotube arrays have poor antibacterial ability and are not effective against implant infection. In this study, TiCu coatings with different Cu content were deposited on pure Ti substrates by magnetron sputtering technique, then Cu was successfully doped into TiO_2 nanotube arrays by anodic oxidation. The long-term antibacterial properties of Cu-doped TiO_2 nanotube arrays were studied. Cytocompatibility and its effects on endothelial cells and osteoblasts. The results show that the doping amount of Cu in TiCu composite coating with different Cu content deposited on pure Ti surface by magnetron sputtering technique can be adjusted between 0 at.% and 20. 04at.%) when the atomic percentage of Cu in TiCu coating is less than 15. 14? A highly ordered Cu-Ti-O nanotube array can be constructed by anodic oxidation, when the percentage of Cu atoms in the TiCu coating is 20.04km. The prepared nanostructured cupric copper ion (CuP2), which was continuously released from the nanoporous structure of Ti-Ti-O nanotube arrays, showed a good long-term antibacterial ability against Gram-positive Staphylococcus aureus.) all nanotube arrays adhered to endothelial cells on the surface of the nanotube arrays, and all nanotube arrays exhibited a long term antibacterial activity against S. aureus, and all nanotube arrays adhered to the endothelial cells on the surface of the nanotubes. The effect of Cu on the activity of endothelial cells was not significant, and Cu doping could promote the proliferation of endothelial cells. Compared with TiO_2 nanotube array (control group), the surface of Cu-Ti-O nanotube array and its ion extracts could up-regulate the synthesis of nitric oxide (no) and the secretion of vascular endothelial growth factor (VEGF) in endothelial cells. Moreover, the vascularization ability of endothelial cells cultured with ion extract of Cu-Ti-O nanotube array in vitro was also increased, and the activity of alkaline phosphatase (ALP) of osteoblasts was not significantly affected by Cu-Ti-O nanotube array, and early differentiation was induced. However, Cu-Ti-O nanotube array can promote the deposition of type I collagen protein and inhibit the mineralization of extracellular matrix, but with the prolongation of induction and culture time, Cu-Ti-O nanotube array can promote the deposition of type I collagen protein and inhibit the mineralization of extracellular matrix. This phenomenon becomes less and less obvious and even disappear. Therefore, good antibacterial properties, bone formation and angiogenesis make Cu-Ti-O nanotube arrays a promising coating for titanium implants.
【学位授予单位】：太原理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;R318.08

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