超支化聚缩水甘油表面接支的磁性纳米粒子的制备方法及性能研究

发布时间：2018-05-09 04:11

本文选题：聚缩水甘油 + 磁性纳米粒子　；参考：《天津理工大学》2015年硕士论文

【摘要】：磁性纳米四氧化三铁具有特殊的理化性质,因此有关其制备和应用成为纳米材料领域日渐关注的一个研究热点。超支化聚缩水甘油接枝的磁性Fe3O4纳米粒子(HPG-grafted MNPs)由于其杰出的生物惰性和表面可修饰性已被广泛研究,本论文着重对其合成方法和其作为一种新的磁共振成像(MRI)造影剂的潜在医学应用等方面进行了探索。在本论文中,我们通过一锅法分别以乙酰丙酮铁,草酸铁为前体成功的合成出HPG-grafted MNPs。所合成的HPG-grafted MNPs的粒径可通过调节其前体的浓度进行调节。HPG-grafted MNPs的表面化学态和晶核形态分别进行了X射线光电子能谱(XPS)和X射线衍射(XRD)表征。使用凝胶色谱(GPC),热重分析(TGA),核磁共振(1 H NMR)对HPG-grafted MNPs中表面有机组分的分子量,含量与结构进行表征。纳米颗粒的大小及形态通过透射电子显微镜(TEM)进行了表征。使用动态光散射(DLS)对纳米粒子的动态尺寸进行测定,结果表明HPG-grafted MNPs可高度分散于水相介质中,例如在细胞培养液和血清中都有极好分散性。尤其是平均粒径为8 nm的粒子更是可以以高达45mg/ml的浓度均匀稳定地分散在水相介质中。体外细胞毒性实验(MTT)表明HPG-grafted MNPs对于巨噬细胞和3T3成纤维细胞不具有显著毒性。随后使用振动样品磁强计(VSM)对HPG-grafted MNPs的磁学性质进行了表征,通过分析所得磁通量曲线得出结论:HPG-grafted MNPs具有较高的饱和磁通量和超顺磁性。然后,我们又以HPG-grafted MNPs的粒径为变量考察了其粒径大小对饱和磁通量、横向弛豫(R 2)与R 2/R1比值、细胞毒性、细胞吞噬量等实验结果的影响。最后,我们进行了该纳米粒子的初步的动物体内核磁造影实验,观察了HPG-grafted MNPs在白兔体内的分布情况和造影能力。
[Abstract]:Magnetic nanocrystalline ferric oxide has special physical and chemical properties, so the preparation and application of magnetic nanocrystalline ferric oxide has become a hot research topic in the field of nanomaterials. Hyperbranched polyglycidyl grafted magnetic Fe3O4 nanoparticles have been widely studied for their outstanding biological inertia and surface modifiability. In this paper, the synthetic method and its potential medical application as a new MRI contrast agent are explored. In this thesis, we successfully synthesized HPG-grafted MNPs with iron acetylacetone and ferric oxalate as precursors by one pot method. The particle size of the synthesized HPG-grafted MNPs was characterized by X-ray photoelectron spectroscopy (XPS) and X-ray diffraction X-ray diffraction (XRD), respectively, by adjusting the concentration of the precursor. The surface chemical state and the nucleation morphology of the prepared MNPs were characterized by X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), respectively. The molecular weight, content and structure of the components on the surface of HPG-grafted MNPs were characterized by gel chromatography, thermogravimetric analysis and 1H NMR. The size and morphology of nanoparticles were characterized by TEM. Dynamic light scattering (DLS) was used to determine the dynamic size of nanoparticles. The results showed that HPG-grafted MNPs could be highly dispersed in aqueous media, such as in cell culture medium and serum. In particular, particles with an average diameter of 8 nm can be uniformly and stably dispersed in aqueous media with concentrations as high as 45mg/ml. In vitro cytotoxicity test (MTT) showed that HPG-grafted MNPs was not significantly toxic to macrophages and 3T3 fibroblasts. The magnetic properties of HPG-grafted MNPs were then characterized by vibratory sample magnetometer. The magnetic flux curves obtained from the analysis showed that the high saturation magnetic flux and superparamagnetism of the MNPs were obtained by the analysis of the magnetic flux curves. Then, the effects of the particle size of HPG-grafted MNPs on the saturation magnetic flux, the ratio of transverse relaxation R _ 2) to R 2/R1, cytotoxicity and phagocytosis were investigated. Finally, the distribution and ability of HPG-grafted MNPs in rabbits were observed by nuclear magnetic resonance imaging (NMR) in vivo of the nanoparticles.
【学位授予单位】：天津理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ316.343;TB383.1

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