超顺磁性氧化铁纳米粒子的制备及其磁共振成像研究

发布时间：2018-05-09 08:50

本文选题：氧化铁纳米粒子 + 高温热分解　；参考：《桂林理工大学》2015年硕士论文

【摘要】：本文选用的铁源为乙酰丙酮铁(Fe(acac)3),通过高温热分解法制备超顺磁性氧化铁纳米粒子(SPIONs)。利用X射线衍射分析(XRD)和透射电子显微镜(TEM)对样品的晶相结构及形貌进行表征,通过傅里叶红外光谱仪对样品的表面修饰的有机物层进行分析,使用马尔文激光粒度仪测量样品的zeta电位值和水合动力学粒径,样品的磁性能通过SQUID来分析,采用感应耦合等离子体光谱仪进行测定Fe的浓度,通过磁共振成像仪分析样品的在昆明(KM)小鼠脑部的磁共振成像(MRI)效果。主要的研究内容如下:以PEG为溶剂,聚乙烯亚胺(PEI)为添加剂制备的PEG和PEI共同修饰的SPIONs(PEG/PEI-SPIONs)。研究结果表明所合成的PEG/PEI-SPIONs的粒径为10.3±1.9 nm。经红外光谱分析表明,PEG和PEI均包覆在氧化铁纳米粒子表面,其具有较高的稳定性和超顺磁性,饱和磁化强度为50.4 emu/g,能够在水中保持3个月以上的稳定性,同时也能在磷酸缓溶液(PBS)中保持较好的稳定性,PEG/PEI-SPIONs在KM小鼠脑部具有良好的MRI造影效果。将PEG/PEI-SPIONs与吐温80(Tween 80)进行进一步接枝,接枝后得到PEG/PEI/Tween 80-SPIONs,其水合动力学粒径由21.0 nm变为24.0 nm,zeta电位值由35.0 mV变为19.0 mV,经红外分析表明,PEG、PEI及Tween 80共同包裹在氧化铁纳米粒子表面,其具有很高的稳定性及超顺磁性,能够在水和PBS中保持较好的稳定性,PEG/PEI/Tween 80-SPIONs在KM小鼠脑部具有一定的MRI造影效果。将合成的PEG/PEI-SPIONs进一步包覆聚天冬氨酸(PASP),包覆PASP后得到PEG/PEI/PASP-SPIONs,其水合动力学粒径由21 nm变为23.4 nm,zeta电位值由35 mV变为-20.4 mV,红外分析表明PEG、PEI及PASP共同修饰在氧化铁纳米粒子表面,其具有很高的稳定性及超顺磁性,PEG/PEI/PASP-SPIONs在KM小鼠脑部的磁共振成像具有一定的造影效果。以PEG为溶剂,0和0.3 g PVP为添加剂制备的PEG和PEG/PVP修饰的SPIONs(PEG-SPIONs、PEG/PVP-SPIONs)。进一步接枝N-(β-氨乙基-β-氨丙基)甲基二甲氧基硅烷(AEAPS)或马来酸酐(Mal),接枝Mal后PEG-SPIONs的再分别与转铁蛋白(Tf)、多肽(Angiopep-2,ANG)进行连接,成功制备得到由PEG/Mal/Tf、PEG/Mal/ANG修饰的SPIONs(PEG/Mal/Tf-SPIONs、PEG/Mal/ANGSPIONs)。
[Abstract]:In this paper, the superparamagnetic ferric oxide nanoparticles (SPION) were prepared by high temperature thermal decomposition of Tie Yuan. The crystal structure and morphology of the samples were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The surface modified organic layer was analyzed by Fourier transform infrared spectroscopy (FTIR). The zeta potential and the particle size of hydration kinetics were measured by Ma Erwen laser particle size analyzer. The magnetic properties of the samples were analyzed by SQUID, and the concentration of Fe was determined by inductively coupled plasma spectrometer. Magnetic resonance imaging (MRI) was used to analyze the effect of magnetic resonance imaging (MRI) in the brain of Kunming KM mice. The main research contents are as follows: SPIONs / PEI-SPIONs modified by PEG and PEI were prepared by using PEG as solvent and polyethyleneimine (PEI) as additive. The results show that the particle size of the synthesized PEG/PEI-SPIONs is 10.3 卤1.9 nm. The infrared spectrum analysis shows that both PEG and PEI are coated on the surface of iron oxide nanoparticles, which have high stability and superparamagnetism, and the saturation magnetization is 50.4 emu / g, which can keep the stability in water for more than 3 months. At the same time, the stability of PEG / PEI-SPIONs in the brain of km mice was better than that of PBSs in slow solution of phosphoric acid. The results showed that PEG / PEI-SPIONs had a good effect on brain MRI in km mice. PEG/PEI-SPIONs was grafted with Twain 80(Tween 80), and PEG/PEI/Tween 80-SPIONs were grafted. The particle size of PEG/PEI/Tween 80-SPIONs was changed from 21.0 nm to 24.0 nm from 35.0 MV to 19.0 MV. Ir analysis showed that PEG/PEI/Tween 80-SPIONs were encapsulated on the surface of iron oxide nanoparticles. It has high stability and superparamagnetism, and can maintain good stability in water and PBS. PEG / PEI / Tween 80-SPIONs has a certain effect of MRI imaging in brain of km mice. The synthesized PEG/PEI-SPIONs was further coated with polyaspartic acid (PASPN) and coated with PASP to obtain PEG / PEI / PASP-SPIONs. The particle size of PEG / PEI / PASP-SPIONs changed from 21 nm to 23.4 nm Zeta potential from 35 MV to -20.4 MV. Ir analysis showed that PEGN PEI and PASP were co-modified on the surface of iron oxide nanoparticles. It has high stability and magnetic resonance imaging of PEG / PEI / PASP-SPIONs in km mice. SPIONs modified by PEG and PEG/PVP were prepared with PEG as solvent 0 and 0. 3 g PVP as additives. Further grafted N-( 尾 -aminoethyl- 尾 -aminopropyl) methyl dimethoxysilane (AEAPS) or maleic anhydride (maleic anhydride), and then grafted with Mal, the PEG-SPIONs was connected with transferrin (TfN) and polypeptide Angiopep-2ANGseparately.
【学位授予单位】：桂林理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;TQ138.11

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