静电纺丝法制备纳米纤维膜及其纤溶功能化应用研究

发布时间：2018-05-14 21:16

本文选题：静电纺丝 + 纳米纤维膜　；参考：《苏州大学》2015年硕士论文

【摘要】：本论文主要是基于静电纺丝技术,制备了具有纤溶功能的纳米纤维膜材料。一方面,制备了表面含有ε-赖氨酸配体的功能纳米纤维膜,通过纤维膜材料表面固定的ε-赖氨酸配体特异性结合人体纤溶系统中的核心蛋白质血纤维蛋白溶酶原(Plg)和组织型血纤维蛋白溶酶原激活剂(t-PA),并利用电纺纤维膜大的比表面积和高的孔隙率实现了材料对Plg和t-PA的高负载量,从而有效溶解材料表面形成的初生血栓,提高了材料的血液相容性。另一方面,通过同轴静电纺丝方法,制备了内芯中负载t-PA的“核-壳”结构纳米纤维,以获得可长时间持续释放t-PA的血液接触材料。研究内容如下:1.基于静电纺丝技术,提出了一种于材料表面方便负载t-PA的方法。我们首先设计合成了一种ε-赖氨酸配体修饰的PVA(PVA-Lys),然后通过静电纺丝技术一步法便制备了ε-赖氨酸配体功能化的PVA/PVA-Lys纤维膜。该纤维膜能够很好地抑制非特异性蛋白纤维蛋白原(Fg)的吸附并有着良好的生物相容性,通过纤维膜上ε-赖氨酸配体对t-PA之间的特异性亲和力和电纺膜大的比表面积特点有效地实现了t-PA的负载。血浆复钙化实验表明,纤维膜上t-PA的释放可有效溶解初生血栓。该方法最大的特点是:通过在电纺过程中,简单地改变电纺溶液中PVA-Lys的加入量,就可以方便地调控t-PA在纤维膜上的负载量。2.基于席夫碱反应,提出了一种适用于多种基材表面的ε-赖氨酸配体功能化改性方法。受醛基和氨基之间的席夫碱反应,可形成稳定聚合物凝胶的启发,我们首先设计合成了两种侧链上分别带有醛基的均聚物P(-CHO)和氨基的ε-赖氨酸共聚物P(-NH2),并以乙酸纤维素(CA)为模型基材,利用静电纺丝技术制备了CA的纳米纤维膜,然后将CA纤维膜依次浸泡在P(-CHO)和P(-NH2)的水溶液中,通过这种简单的温和的水相浸泡法,就可得到含有ε-赖氨酸残基聚合物凝胶层改性的纳米纤维膜。利用ε-赖氨酸配体和Plg之间的特异性亲和力及电纺膜大的比表面积效应,其能够大量吸附血浆中的Plg,从而简便地实现了具有潜在纤溶功能材料表面的构建。3.基于同轴静电纺丝方法,以具有良好生物相容性和可降解性的CA为外层材料,制备了内芯中负载t-PA的“核-壳”结构纳米纤维。TEM测试表明t-PA位于纤维的内层,Bradford法测试结果显示纤维膜中负载的t-PA能够长时间的持续缓慢释放。
[Abstract]:In this paper, the fiber membrane materials with fibrinolytic function were prepared based on the electrostatic spinning technology. On the one hand, the functional nanofiber membrane containing the epsilon lysine ligand was prepared. The protein lyzase, the core protein of the human fibrinolytic system, was specifically combined with the epsilon lysine ligands fixed on the surface of the fiber membrane material. The original (Plg) and the tissue type plasminogen activator (t-PA), and the high specific surface area and high porosity of the electrospun fiber membrane, have realized the high load of the material to Plg and t-PA, thus effectively dissolving the primary thrombus formed on the surface of the material and improving the blood compatibility of the material. On the other hand, the coaxial electrospinning method is made. A "nuclear shell" nano fiber loaded with t-PA in the inner core was prepared to obtain a blood contact material that could release t-PA for a long time. 1. based on the electrostatic spinning technology, a method of convenient loading of t-PA on the surface of the material was proposed. First, we designed and synthesized a PVA (PVA-Lys) modified by an epsilon lysine ligand. The functionalized PVA/PVA-Lys fiber membrane of the epsilon lysine ligand is prepared by one step method of electrostatic spinning. The membrane can effectively inhibit the adsorption of non specific protein fibrinogen (Fg) and have good biocompatibility. The specific affinity of the t-PA and the electrospun membrane are large through the epsilon lysine ligand on the fiber membrane. The load of t-PA is effectively realized by the specific surface area characteristics. The experiment of plasma calcification shows that the release of t-PA on the fiber membrane can effectively dissolve the primary thrombus. The greatest feature of this method is that by simply changing the amount of PVA-Lys added in the electrospun solution during the electrospinning process, the.2. base on the load of t-PA in the fiber membrane can be easily regulated. In the Schiff base reaction, a functional modification method of the epsilon lysine ligand suitable for various substrates is proposed. Inspired by the reaction between the aldehyde group and the amino group, the stability of the polymer gel can be formed. We first designed and synthesized two kinds of epsilon lysine copolymers with aldehyde based homopolymer P (-CHO) and amino group on the side chain, respectively. P (-NH2), and using cellulose acetate (CA) as the model base material, the nano fiber membrane of CA was prepared by electrostatic spinning technology, and then the CA fiber membrane was soaked in P (-CHO) and P (-NH2) water solution in turn. By this simple mild water immersion method, the nanofiber membrane containing the polymer gel layer of epsilon lysine residue polymer could be obtained. With the specific affinity between the epsilon lysine ligand and Plg and the large specific surface area effect of the electrospun membrane, it can adsorb a large amount of Plg in the plasma, thereby simply realizing the construction of.3. based on the surface of the potential fibrinolytic material, which is based on the coaxial electrospinning method, with good biocompatibility and degradability of CA as the outer material. The nuclear shell structure nanofiber.TEM test of the load t-PA in the inner core showed that t-PA was located in the inner layer of the fiber. The test results of Bradford method showed that the t-PA of the load in the fiber membrane could be released slowly and slowly for a long time.

【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.2;TQ340.1

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