环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性

发布时间：2018-05-17 13:46

本文选题：姜黄素 + β-环糊精　；参考：《食品科学》2017年21期

【摘要】：采用纳米银材料制备负载姜黄素(curcumin,Cur)的β-环糊精(β-cyclodextrin,β-CD)功能化纳米银——Ag@β-CD@Cur,并进一步对其抗肿瘤活性进行测定。运用透射电子显微镜和纳米粒度仪鉴定Ag@β-CD@Cur的表面形貌、粒径大小和稳定性。运用倒置荧光显微镜和3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法检测Ag@β-CD@Cur对人肝癌细胞HepG2形态和活性的影响,并进一步采用流式细胞术检测细胞凋亡比例。实验结果表明,所制备的Ag@β-CD@Cur是纯度很高的单质银,且大小均一、表面光滑,粒径约为2 nm。同时,Ag@β-CD@Cur具有较好的稳定性,能在水中稳定存在30 d。经Ag@β-CD@Cur处理HepG2细胞24 h后,HepG2细胞的形态出现明显的变圆、细胞核固缩等细胞凋亡特征,同时,细胞存活率从对照组的100%降低到24%,进一步的流式细胞术分析结果表明,细胞凋亡比例从对照组的2.5%增加到51.0%。实验结果表明,纳米化后的Cur具有较强的抗肿瘤活性效果,这为Cur在纳米制剂的应用提供了一定的技术手段和理论数据参考。
[Abstract]:尾 -cyclodextrin (尾 -CDN) -functionalized nano-silver (Ag@ 尾 -CD-protein CurCurin) loaded curcumin (Curcumin) was prepared and its antitumor activity was further determined. The surface morphology, particle size and stability of Ag@ 尾 -CD-Cur were characterized by transmission electron microscope (TEM) and nano-particle size analyzer. The effects of Ag@ 尾 -CDCur on the morphology and activity of human hepatoma cell line (HepG2) were detected by inverted fluorescence microscope and 3-chloride-5-dimethylthiazole-2-diphenyl tetrazolium bromide method, and the apoptosis ratio was determined by flow cytometry. The results show that Ag@ 尾 -CD@ curl is a pure silver with uniform size, smooth surface and a particle size of about 2 nm. At the same time, Ag@ 尾 -CD-Cur has good stability and can exist in water for 30 days. After treated with Ag@ 尾 -CDC-Cur for 24 h, the morphology of HepG2 cells became round obviously, the cell nuclear pyknosis and other apoptotic characteristics, and the cell survival rate decreased from 100% in the control group to 24% in the control group. The results of flow cytometry showed that the cell viability of HepG2 cells decreased from 100% in the control group to 24% in the control group. The percentage of apoptosis increased from 2.5% in the control group to 51.0% in the control group. The experimental results show that the nano-sized Cur has a strong anti-tumor effect, which provides a certain technical means and theoretical data reference for the application of Cur in nano-preparations.
【作者单位】：广东海洋大学食品科技学院;广东省天然产物绿色加工与产品安全重点实验室;江西省粮油质量监督检验中心;
【基金】：国家自然科学基金青年科学基金项目(21602034) 广东省自然科学基金博士启动项目(2016A30310332) 广东省公益研究与能力建设项目(2015A020209166) 广东省天然产物绿色加工与产品安全重点实验室开放基金项目(201617)
【分类号】：R283.6;TB383.1

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