卵清溶菌酶模板法组装贵金属纳米粒子链

发布时间：2018-06-06 12:18

本文选题：生物模板 + 卵清溶菌酶纤维　；参考：《燕山大学》2015年硕士论文

【摘要】：卵清溶菌酶是一种单股肽链的碱性蛋白,在强酸中可以稳定性的存在,但是当加热到一定温度时便可以发生结构变化,即形成网状交错的纳米纤维。卵清溶菌酶由于其自身的优点引起了许多研究学者的兴趣,现阶段国内外以卵清溶菌酶为模板组装金属纳米粒子链的很少。本论文选用卵清溶菌酶纤维为生物模板,通过TEM、UV、EDS、SAED、XRD、CV表征手段对样品进行分析表征,本文主要研究了Pt纳米粒子链、Rh纳米粒子链、Au纳米粒子链的组装。主要完成内容如下:1.通过TEM表征确定卵清溶菌酶及其纤维形貌结构。2.通过讨论不同条件下的对照实验探究出制备铑纳米粒子链的最佳条件,并对其结果进行分析,成功的制备出铑纳米粒子链,纤维化4天组装的铑纳米粒子链具有两种形态,一种是长在卵清溶菌酶纤维里面,其铑纳米粒子链的直径为30nm,一种是长满卵清溶菌酶纤维,其铑纳米粒子链的直径为40nm。纤维化4天时组装的铑纳米粒子链直径为13nm,同时确定铑纳米粒子链具有一定的催化活性。3.通过讨论卵清溶菌酶的纤维化时间及p H条件确定最优条件组装铂的纳米粒子链,并通过EDS和SAED确定其属于多晶型金属;4.成功合成金纳米粒子链。考察了不同浓度的卵清溶菌酶纤维、加入化学体系后p H值及加入化学体系的溶液量对组装金纳米粒子链的影响。同时又通过改变其纤维化时间及最后阶段的加热条件来调控组装的金纳米粒子链的形貌,又通过MTT测试确定金纳米粒子链具有较好的生物相容性,其在生物医学界的应用具有很大的潜在价值。
[Abstract]:Egg white lysozyme is a kind of basic protein with single strand peptide chain, which can be stable in strong acid. However, when heated to a certain temperature, the structure of egg white lysozyme can change, that is to say, it forms a network of interlaced nanofibers. Ovalbum lysozyme has attracted the interest of many researchers because of its own advantages. At present, few metal nanoparticles chains are assembled by using ovalbumin as template at home and abroad. In this paper, the ovalbum lysozyme fiber was selected as the biological template, and the samples were characterized by TEMUVUVSEDSSAEDX XRDX CV. In this paper, the assembly of au nanocrystalline chains of Pt nanocrystalline chain Rh nanoparticles was studied. The main contents are as follows: 1. The morphology and structure of lysozyme and its fiber were determined by TEM. The optimal conditions for the preparation of rhodium nanocrystalline chains were investigated by discussing the control experiments under different conditions and the results were analyzed. The rhodium nanoparticles chains were successfully prepared. The rhodium nanoparticles chains assembled in 4 days of fibrosis had two forms. The diameter of rhodium nanoparticle chain is 30 nm and the diameter of rhodium nanocrystalline chain is 40 nm. The diameter of the rhodium nanoparticles was 13 nm after 4 days of fibrosis. At the same time, it was determined that the rhodium nanoparticles chain had a certain catalytic activity. 3. By discussing the fibrosis time of ovalbum lysozyme and pH conditions, the optimal conditions for assembling platinum nanoparticles chain were determined, and the polycrystalline metal chain was determined by EDS and SAED. Gold nanoparticles chain was successfully synthesized. The effects of different concentrations of ovalbumin lysozyme fibers, pH value and the amount of solution added into the chemical system on the chain of gold nanoparticles were investigated. At the same time, the morphology of the assembled gold nanoparticles was regulated by changing the time of fibrosis and the heating conditions of the final stage, and the biocompatibility of the gold nanoparticles was determined by MTT test. Its application in biomedical field has great potential value.
【学位授予单位】：燕山大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1

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