新型荧光纳米材料的制备及其应用研究

发布时间：2018-06-08 08:25

本文选题：稀土纳米粒子 + 上转换荧光　；参考：《南京师范大学》2015年硕士论文

【摘要】：积极地寻找性能优异的光致化学发光新材料,成为研发基于光致化学发光技术的高灵敏生物传感器和高清晰生物成像的关键。本论文以稀土上转换发光纳米材料和碳量子点为研究对象,对这两类材料的制备新技术及生物医学应用进行了探索,具体研究结果如下：(1)要实现对生物分子的高灵敏检测,就要使稀土上转换发光纳米材料具备良好的水溶性、生物相容性、高的荧光发射量子产率和多色光发射等多功能化特性。本论文以氨基酸作为有机包裹剂,合成了由30nm左右大小稀土上转换纳米粒子自组装形成的稻草束状超结构YF3:Yb3+, Er34颗粒,探讨了氨基酸以及氟源在形貌形成过程中的作用；此外,实验当中通过掺杂过渡金属铜元素的方法,在保持稻草束状结构基本不变的前提下,得到了铜掺杂的六方β-NaYF4: Yb3+, Er3+上转换荧光纳米粒子,并且发现随着铜元素掺杂比例的提高,稀土上转换荧光纳米材料的荧光强度也随之增强,当掺杂比例为40%时,得到最大的荧光强度；将上述制备得到的铜掺杂NaYF4:Yb3+.上转换荧光纳米粒子应用到谷胱甘肽的检测当中,达到了40 nM的检测限。(2)以不同氨基酸为碳源,通过水热法制备了具有良好水溶性和较高荧光量子产率的碳量子点。对其进行了X射线衍射表征和拉曼光谱表征,确认得到的碳量子点主要成分为无定形碳。通过XPS表征数据和傅里叶红外表征数据,进一步得知其中碳原子和氮原子的成键方式。当使用不同的激发光对碳量子点溶液进行激发之后,其发射峰的位置会随着激发光位置的改变而改变,即具有" Excitation-Dependent"性质。在使用396nm光源激发下,可得到碳量子点荧光的最强值。细胞毒性检测试验表明该类碳量子点细胞毒性较低,与Hela细胞培养24h后,使用相关激发光源进行激发,发现细胞呈现较好的形态,并且能够发出绿色荧光,这为该类碳量子点未来应用于其他生物成像及荧光防伪领域提供了可能。
[Abstract]:Actively looking for new photochemiluminescence materials with excellent performance has become the key to the development of high sensitivity biosensor and high resolution biometric imaging based on photoluminescence technology. In this paper, the upconversion luminescent nanomaterials and carbon quantum dots (QDs) of rare earths are taken as the research objects, and the preparation technology and biomedical application of these two kinds of materials are explored. The specific results are as follows: 1) High sensitive detection of biomolecules should be realized. It is necessary to make rare earth up-conversion luminescent nanomaterials with good water-solubility, biocompatibility, high fluorescence emission quantum yield and multi-color emission. In this paper, the rice straw bunchy superstructure YF3: Yb3, Er34 particles were synthesized by using amino acids as organic encapsulating agent, and the effects of amino acids and fluorine sources on morphology formation were discussed. In the experiment, the copper doped hexagonal 尾 -NaYF4: Yb3, Er3 up-conversion fluorescent nanoparticles were obtained by doping the transition metal copper and keeping the straw beam structure basically unchanged, and it was found that with the increase of the doping ratio of copper, The fluorescence intensity of rare earth up-conversion fluorescent nanomaterials also increased, and the maximum fluorescence intensity was obtained when the doping ratio was 40%, and the copper doped NaYF _ 4: Yb _ 3 was obtained. Up-conversion fluorescent nanoparticles were applied to the detection of glutathione, reaching the detection limit of 40 nm. (2) carbon quantum dots with good water solubility and high fluorescence quantum yield were prepared by hydrothermal method using different amino acids as carbon source. X-ray diffraction and Raman spectroscopy were used to characterize the QDs. The main components of the QDs were identified as amorphous carbon. The bonding modes of carbon and nitrogen atoms were further obtained by XPS and Fourier transform infrared spectroscopy. When different excitation light is used to excite the carbon quantum dot solution, the position of the emission peak will change with the change of the excited light position, that is, it has the property of "Excitation-Dependent". The strongest fluorescence value of carbon quantum dots can be obtained when excited by 396nm light source. The cytotoxicity test showed that the cytotoxicity of this kind of QDs was low. After cultured with Hela cells for 24 hours, the cells were stimulated with the related excitation light source, and the cells were found to be in good shape and could emit green fluorescence. This provides the possibility for the future application of this kind of carbon quantum dots in other biological imaging and fluorescence anti-counterfeiting fields.
【学位授予单位】：南京师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O657.3;TB383.1

【相似文献】