基于纳米粒子的功能化技术用子细胞内小分子活性物质的检测

发布时间：2018-07-03 15:52

本文选题：金纳米粒子 + ATP　；参考：《青岛科技大学》2015年硕士论文

【摘要】：第一章,对相关技术背景进行了详细地介绍,比如金纳米粒子的制备及其在生物医学中的应用、DNA、肿瘤细胞标志物、电致化学发光、荧光及激光共聚焦显微镜等,了解了目前研究的现状及检测的技术水平,对自已研究的课题具有指导意义。最后介绍了本课题的研究目的及研究内容。第二章,制备金纳米粒子(AuNRs),并且以金纳米粒子作为标记物,能够和多种生物分子结合。通过对DNA的一些处理,建立了金纳米粒子的功能化核酸适体的体系来检测溶液中的ATP含量,并将此体系来与细胞结合并进一步检测细胞内ATP的含量。检测限为4.0×10.8 M浓度的ATP,并可以检测200-5000个Ramos细胞。第三章,以合成的硫化镉(CdS)量子点为基础,本章设计了一个基于CdS传感器的电致化学发光法和荧光分光光度法来检测ATP的含量的方法,对ATP有快速的响应。当体系中存在ATP时,它就会和ATP适体链结合,从而释放出CdS-DNA’链,CdS在包含0.1 M KC1和0.05 M K2S208的PBS缓冲溶液中能够产生ECL信号,通过ECL强度的改变,确定ATP的浓度。优化实验条件后,此传感器可以对2.0×10-9-1.0×10-7 M浓度范围内的ATP有响应。也可以在金片上进行修饰,就可以用于荧光的测量,对2.0×10.9-1.0×10-7M浓度范围内的ATP同样有响应,因此此传感器在生物分析领域有着很好的应用前景。
[Abstract]:In the first chapter, the related technical background is introduced in detail, such as the preparation of gold nanoparticles and their applications in biomedical applications, such as DNA, tumor cell markers, electrochemiluminescence, fluorescence and laser confocal microscopy, etc. The present situation of research and the technical level of detection are understood, which is of guiding significance to the subject of our own research. Finally, the purpose and content of the research are introduced. In chapter 2, gold nanoparticles (AuNRs) were prepared, and gold nanoparticles were used as markers to bind to a variety of biomolecules. Through the treatment of DNA, a system of functional aptamer of gold nanoparticles was established to detect the ATP content in the solution, and the system was used to bind to the cells and further detect the ATP content in the cells. The detection limit was 4.0 脳 10.8 M, and 200-5000 Ramos cells could be detected. In chapter 3, based on the synthesized cadmium sulphide (CDs) quantum dots, we design a new method to detect ATP content by electrochemiluminescence and fluorescence spectrophotometry based on CDs sensor, which has a rapid response to ATP. When ATP is present in the system, it binds to the aptamer chain of ATP, thus releasing CdS-DNA 'chain CDs can produce ECL signal in PBS buffer solution containing 0.1 MKC1 and 0.05 MK2S208. The concentration of ATP is determined by changing the intensity of ECL. After optimizing the experimental conditions, the sensor can respond to ATP in the concentration range of 2.0 脳 10-9-1.0 脳 10-7 M. It can also be modified on a gold chip and can be used for fluorescence measurement. It is also responsive to ATP in the concentration range of 2.0 脳 10.9-1.0 脳 10 ~ (-7) M, so this sensor has a good application prospect in the field of biological analysis.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;TP212

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