暴露纳米氧化锌对雄性小鼠糖、脂代谢及组织学的影响

发布时间：2018-07-03 17:50

本文选题：纳米氧化锌 + 血脂　；参考：《中国兽医学报》2017年03期

【摘要】：根据小鼠的体质量,以不同剂量经口连续染毒纳米氧化锌,90d后摘眼球取血,对血脂、血糖、肝脏抗氧化指标进行检测。结果显示:对照组、80mg/kg剂量组小鼠血糖显著高于160mg/kg剂量组(P0.05)。对照组、80mg/kg剂量组小鼠血糖极显著高于40mg/kg剂量组(P0.01);40,80 mg/kg剂量组小鼠血脂含量显著高于对照组(P0.05),160mg/kg剂量组小鼠血脂含量极显著高于对照组(P0.01);40,160 mg/kg剂量组小鼠血清总胆固醇含量显著高于对照组(P0.05);40mg/kg剂量组小鼠血清低密度脂蛋白含量显著高于对照组、80mg/kg剂量组(P0.05),各组间血清高密度脂蛋白含量无显著差异;80,160mg/kg剂量组小鼠肝脏过氧化氢酶活性显著高于对照组、40mg/kg剂量组(P0.05),80mg/kg剂量组小鼠肝脏过氧化氢酶活性极显著高于40 mg/kg剂量组(P0.01);40,80mg/kg剂量组小鼠肝脏Cu-Zn SOD活性显著高于对照组(P0.05);160 mg/kg剂量组小鼠肝脏抑制羟自由基能力显著低于对照组、40mg/kg剂量组及80mg/kg剂量组(P0.05);随染毒剂量增加,小鼠肝脏MDA含量增加,但各组间含量差异不显著;随染毒剂量增加,小鼠肝脏谷胱甘肽过氧化物酶活性提高;但各组间活性差异不显著。各组间肝脏H2O2含量、总SOD活性无显著性差异。病理学检查发现,大脑细胞存在噬神经现象,脾脏红髓与白髓的界限不清晰;肺泡壁增厚,局部炎性细胞浸润,曲精小管间的间隙增大,生精细胞排列紊乱、脱落。结果表明:经口连续染毒纳米氧化锌90d能影响雄性小鼠肝脏抗氧化系统,导致小鼠糖、脂代谢紊乱,引起高脂血症,纳米氧化锌对雄性小鼠具有神经毒性及生殖毒性。
[Abstract]:According to the body weight of mice, blood lipid, blood glucose and liver antioxidation index were detected after 90 days of continuous oral administration of nanometer zinc oxide. The results showed that the blood glucose in the control group (80 mg / kg) was significantly higher than that in the 160mg/kg group (P0.05). The blood glucose content of the mice in the control group (80 mg / kg) was significantly higher than that in the 40mg/kg group (P0.01) and that in the 4080 mg/kg group was significantly higher than that in the control group (P0.05). The blood lipid content of the mice in the control group (P0.01) was significantly higher than that in the control group (P0.01) and the serum total cholesterol content of the mice in the 40160 mg/kg group was significantly higher than that in the control group (P0.01). The content of serum low density lipoprotein in 40 mg / kg group was significantly higher than that in 80 mg / kg group (P0.05). There was no significant difference in the content of serum high density lipoprotein in mice of 80160 mg / kg dose group (P0.05). The liver catalase activity of mice in 80160 mg / kg dose group was significantly higher than that in control group (P0.05). The liver catalase activity of the mice in the 80mg / kg group was significantly higher than that in the 40 mg/kg group (P0.01) and the activity of Cu-Zn SOD in the liver was significantly higher than that in the control group (P0.05) and the liver inhibition of hydroxyl free radical activity in the control group (P0.05). The power was significantly lower than that in the control group (40 mg / kg) and 80mg/kg (P0.05). The content of MDA in liver of mice increased, but the content of glutathione peroxidase increased with the increase of dose, but the activity of glutathione peroxidase in each group was not significant. There was no significant difference in H _ 2O _ 2 content and total SOD activity among groups. Pathological examination showed that there was a phenomenon of macrophage in brain cells, the boundary between red and white marrow of spleen was not clear, the alveolar wall was thickened, local inflammatory cells were infiltrated, the gap between seminiferous tubules was enlarged, and the spermatogenic cells were arranged disorderly and dropped off. The results showed that 90 days of continuous oral administration of nano-zinc oxide could affect the liver antioxidant system of male mice, lead to disorder of glucose and lipid metabolism, and induce hyperlipidemia. Nanometer zinc oxide had neurotoxicity and reproductive toxicity to male mice.
【作者单位】：河南科技大学动物科技学院;河南省高等学校重点学科环境与畜产品安全开放实验室;
【基金】：河南科技大学自然科学领域创新能力培育基金资助项目(4025/13350065);河南科技大学SRTP资助项目(2014261)
【分类号】：TB383.1;S865.13

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