聚脲多孔材料的简单制备及在酶固定和手性拆分中的应用

发布时间：2018-07-11 15:01

本文选题：沉淀聚合 + 聚脲多孔材料　；参考：《高等学校化学学报》2017年03期

【摘要】：以甲苯二异氰酸酯(TDI)为单体,在水与丙酮混合溶剂中通过沉淀聚合一步法制备了富含胺基的聚脲多孔材料(PPU),通过扫描电镜和压汞法对其表面形貌和孔结构进行了表征.PPU经戊二醛(GA)活化后用于荧光假单胞菌脂肪酶(PFL)的固定,考察了GA活化过程中GA浓度对酶固定量及固定酶活性的影响.结果表明,PPU是一种粒子尺寸分布在30~50μm范围的形状不规则的多孔粒子,孔径在2 nm~100μm之间呈连续分布.在pH=8.0的缓冲溶液中用0.17 mol/L的GA对PPU进行改性,将改性后的PPU用于PFL的固定,当酶溶液浓度为2.56 mg/m L时,得到酶的最大固定量为95.2 mg/g,固定酶的活性为375 U/mg,相对活性为76%.将此固定酶作为催化剂,用于1-苯乙醇外消旋化合物的手性拆分,并与游离酶催化的结果相比较.结果表明,固定酶的反应活性和立体选择性都明显优于游离酶.通过沉淀聚合制备的聚脲多孔材料在酶固定及手性分子拆分方面具有应用前景.
[Abstract]:Using toluene diisocyanate (TDI) as monomer, Amino-rich polyurea porous materials (PPU) were prepared by precipitation polymerization in aqueous and acetone mixed solvents. The surface morphology and pore structure of PPU were characterized by scanning electron microscopy (SEM) and mercury porosimetry. PPU was activated by glutaraldehyde (GA). Immobilization of lipase from Pseudomonas fluorescens (PFL), The effects of GA concentration on enzyme fixation and enzyme activity during GA activation were investigated. The results show that nm~100 is an irregular porous particle with particle size distribution in the range of 30 ~ 50 渭 m, and its pore size is continuous between 2 nm~100 渭 m. PPU was modified with 0.17 mol / L GA in buffer solution of pH = 8.0. The modified PPU was used for immobilization of PFL. When the concentration of enzyme solution was 2.56 mg/m / L, the maximum immobilization amount was 95.2 mg / g, the activity of immobilized enzyme was 375 U / mg, and the relative activity was 76.5%. The immobilized enzyme was used as a catalyst for the chiral resolution of 1-phenylethanol racemic compounds and the results were compared with those obtained by free enzyme catalysis. The results showed that the activity and stereoselectivity of immobilized enzyme were better than that of free enzyme. Polyurea porous materials prepared by precipitation polymerization have promising applications in enzyme immobilization and chiral molecular resolution.
【作者单位】：济南大学化学化工学院;中国科学院大学;
【基金】：国家自然科学基金(批准号:21274054;21304038;51473066)资助~~
【分类号】：TB383.4

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