水溶性纳米富勒烯衍生物的制备及其对细胞氧化应激保护作用的研究

发布时间：2018-07-11 17:27

本文选题：水溶性富勒烯衍生物 + 氨基　；参考：《化工新型材料》2016年08期

【摘要】：富勒烯C60的缺电子多烯结构,容易与自由基发生加成反应,被称为"自由基的海绵",可保护细胞免受自由基伤害。它的笼形结构还可以用作药物载体,实现靶向释放,因此在生物和医学领域中应用受到广泛的关注。但是,富勒烯本身具有强烈的疏水性,难以在生物介质中直接使用,因此,制备水溶性富勒烯衍生物及提高富勒烯在生物体内的有效摄入量,成为目前研究热点。针对这些问题,设计并制备了水溶性富勒烯羟基氨基多官能度的衍生物,通过氨基的引入,增加了富勒烯在水中溶解度,提高细胞对富勒烯衍生物摄入量。用有机元素分析仪、红外光谱、光电子能谱、热失重等手段对其元素组成和结构进行了表征,其结构式为:C60O~10(OH)~16(NH2)~6(NO2)~6·24H_2O。用激光粒度仪测定其粒径分布和Zeta电势,粒径分布在170nm,Zeta电势为-3.54mV(pH=7.0水溶液)。并探究其对细胞的抗氧化应激的保护作用,结果表明所制备的水溶性富勒烯具有较强的清除超氧阴离子自由基的能力,可使细胞免于氧化应激伤害,提高细胞成活率,并具有浓度依赖性。与富勒醇对比,证明氨基引入确实提高了细胞对富勒烯衍生物的摄入量,为富勒烯在生物医药中应用提供了基础数据。
[Abstract]:The deficiency of the fullerene C60, which is easy to react with free radicals, is called free radical sponges, which can protect cells from free radical damage. Its cage structure can also be used as a drug carrier to achieve target release, so it is widely used in the field of biology and medicine. However, fullerene itself is strong. Strong hydrophobicity is difficult to be used in biological medium directly. Therefore, the preparation of water-soluble fullerenes and the increase of the effective intake of fullerenes have become a hot spot of research. In view of these problems, a derivative of water soluble fullerene hydroxyl amino group is designed and prepared, and the introduction of amino group has increased Fuller. The solubility of alkenes in water increased the intake of fullerenes. The composition and structure of the elements were characterized by organic element analyzer, infrared spectroscopy, photoelectron spectroscopy, and thermal weight loss. The structure of C60O~10 (OH) ~16 (NH2) ~6 (NO2) ~6. 24H_2O. by laser particle size analyzer was used to determine the particle size distribution and Zeta potential and particle size distribution. At 170nm, the potential of Zeta was -3.54mV (pH=7.0 water solution) and the protective effect on the antioxidant stress of cells was explored. The results showed that the prepared water soluble fullerenes had a strong ability to scavenge superoxide anion radicals, which could make cells free from oxidative stress injury, increase cell survival rate, and have concentration dependence. With Fuller alcohol, It is proved that the introduction of amino groups has indeed increased the intake of fullerene derivatives and provided basic data for the application of fullerene in biomedicine.
【作者单位】：北师大附属中学;中国科学院化学所;
【分类号】：O613.71;TB383.1

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