基于纳米材料的毛细管电泳分离检测性能研究及应用
发布时间：2018-08-04 13:54
【摘要】：本论文研究了基于纳米材料的毛细管电泳分离检测性能研究以及其在生物样品分析中的应用。首先,研究了量子点的合成及机理,成功合成了CdSe和巯基乙酸(TGA)包被的CdSe/CdS量子点,并对两种量子点从尺寸上和发光性质方面进行了详细的表征和各种光谱扫描测定。将毛细管电泳与量子点电化学发光分析相结合,研究了量子点的电化学发光性能。在此基础上,研究了生物胺分子对量子点电化学发光的增强作用,构建了毛细管电泳量子点电化学发光分析平台检测生物胺,研究了生物胺催化量子点电化学发光的反应机理。本文还研究了毛细管电泳-酶联免疫分析法检测海洋鱼类样品中CTXIB毒素。通过制作HRP和Ab标记的金纳米粒子作为探针,利用金纳米粒子的放大作用和毛细管电泳电化学检测相结合检测分析了鱼类样品中一种扩增免疫毒素CTXIB。全文共分为四章：第一章,详细介绍了毛细管电泳的基本理论,系统概括了毛细管电泳的基本原理、仪器操作、进样方法的选择等；从不同的角度、各个方面介绍了毛细管电泳的研究现状及应用前景。介绍了量子点的概念以及特点、量子点的制备方法和应用前景,重点介绍了量子点的电化学发光原理和发光方式。对纳米材料的概念及特点作了简要介绍,重点对纳米金及其制备方法作了详细的概述,并介绍了纳米金的应用。第二章,制备了水溶性量子点CdSe和CdSe/CdS合金量子点,利用电镜和光谱仪器对制备的量子点从尺寸上以及发光性质方面进行了表征。探究了影响量子点合成的各种因素以及量子点的合成机理及其结构组成。第三章,探究了基于纳米材料的毛细管电泳量子点电化学发光检测生物胺的研究。包括电化学检测平台的建立,对实验体系中的缓冲溶液的种类、缓冲溶液的pH值、缓冲溶液的浓度、毛细管两端所加的分离电压、所设置的检测电位、毛细管的进样时间、电位扫描速率等因素在内的诸多条件进行了详细优化,确定了最佳检测条件。并在最佳检测条件下,对6种生物胺样品进行了分离检测,不同生物胺分子对量子点电化学发光信号有不同程度的增强作用。第四章,利用纳米金粒子的放大作用通过毛细管电泳和电化学检测相结合分离检测了鱼类样品中一种扩增免疫毒素CTXIB的研究。介绍了纳米金的合成、用辣根过氧化酶和抗体(Ab)修饰纳米金粒子,获得了辣根过氧化酶和抗体标记的纳米金探针,纳米金可以大量吸附游离的HRP和Ab从而使检测的灵敏度大大加强。采用非竞争性免疫分析反应模式,利用电化学检测方法检测了鱼类样品中免疫毒素CTXIB。结论：对前文中所述实验进行了简单的总结概括。
[Abstract]:In this paper, the performance of capillary electrophoresis based on nanomaterials and its application in biological sample analysis were studied. Firstly, the synthesis and mechanism of quantum dots were studied. CdSe/CdS quantum dots coated with CdSe and (TGA) were successfully synthesized. The size and luminescence properties of the two quantum dots were characterized in detail and various spectral scanning measurements were carried out. The electrochemical luminescence properties of quantum dots were studied by combining capillary electrophoresis with electrochemical luminescence analysis of quantum dots. On this basis, the enhancement effect of biogenic amine molecules on electrochemiluminescence of quantum dots was studied. A capillary electrophoresis platform was constructed for the detection of biogenic amines, and the reaction mechanism of biogenic amines catalyzed by biogenic amines was studied. Capillary electrophoresis-enzyme-linked immunosorbent assay (Elisa) for the detection of CTXIB toxin in marine fish samples was also studied. By making gold nanoparticles labeled with HRP and Ab as probes, the amplification of gold nanoparticles and the electrochemical detection of capillary electrophoresis were used to detect and analyze a kind of amplified immunotoxin CTX B in fish samples. The paper is divided into four chapters: the first chapter introduces the basic theory of capillary electrophoresis in detail, systematically summarizes the basic principle of capillary electrophoresis, instrument operation, selection of sampling methods, etc. The present situation and application prospect of capillary electrophoresis were introduced. In this paper, the concept and characteristics of quantum dots, the preparation methods and application prospect of quantum dots are introduced. The electrochemiluminescence principle and luminescence mode of quantum dots are emphatically introduced. The concept and characteristics of nanomaterials are briefly introduced, and the application of nanocrystalline gold is introduced in detail. In chapter 2, water-soluble quantum dots (CdSe) and CdSe/CdS alloy quantum dots (QDs) were prepared. The size and luminescence properties of the QDs were characterized by electron microscopy and spectrometer. The factors affecting the synthesis of quantum dots, the synthesis mechanism and structure composition of quantum dots are discussed. In chapter 3, the electrochemiluminescence (ECL) detection of biogenic amines based on nanomaterials by capillary electrophoresis quantum dots (QDs) was investigated. Including the establishment of electrochemical detection platform, the type of buffer solution in the experimental system, the pH value of buffer solution, the concentration of buffer solution, the separation voltage added at both ends of the capillary, the detection potential set up, the injection time of the capillary, Many factors, such as potential scanning rate, were optimized in detail and the best detection conditions were determined. Six biogenic amines were separated and detected under the best detection conditions. Different biogenic amine molecules enhanced the electrochemiluminescence signal of quantum dots in different degrees. In chapter 4, the amplification effect of gold nanoparticles was used to separate and detect an amplified immunotoxin CTXIB in fish samples by capillary electrophoresis and electrochemical detection. The synthesis of gold nanoparticles was introduced. The gold nanoparticles were modified with horseradish peroxidase and antibody (Ab), and the nano-gold probes labeled with horseradish peroxidase and antibody were obtained. Nanocrystalline gold can adsorb free HRP and Ab in large quantities, so that the sensitivity of detection can be greatly enhanced. The immunotoxin CTXB in fish samples was detected by electrochemical assay using a non-competitive immunoassay model. Conclusion: the experiments mentioned above are summarized briefly.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O658.9
本文编号：2164093
[Abstract]:In this paper, the performance of capillary electrophoresis based on nanomaterials and its application in biological sample analysis were studied. Firstly, the synthesis and mechanism of quantum dots were studied. CdSe/CdS quantum dots coated with CdSe and (TGA) were successfully synthesized. The size and luminescence properties of the two quantum dots were characterized in detail and various spectral scanning measurements were carried out. The electrochemical luminescence properties of quantum dots were studied by combining capillary electrophoresis with electrochemical luminescence analysis of quantum dots. On this basis, the enhancement effect of biogenic amine molecules on electrochemiluminescence of quantum dots was studied. A capillary electrophoresis platform was constructed for the detection of biogenic amines, and the reaction mechanism of biogenic amines catalyzed by biogenic amines was studied. Capillary electrophoresis-enzyme-linked immunosorbent assay (Elisa) for the detection of CTXIB toxin in marine fish samples was also studied. By making gold nanoparticles labeled with HRP and Ab as probes, the amplification of gold nanoparticles and the electrochemical detection of capillary electrophoresis were used to detect and analyze a kind of amplified immunotoxin CTX B in fish samples. The paper is divided into four chapters: the first chapter introduces the basic theory of capillary electrophoresis in detail, systematically summarizes the basic principle of capillary electrophoresis, instrument operation, selection of sampling methods, etc. The present situation and application prospect of capillary electrophoresis were introduced. In this paper, the concept and characteristics of quantum dots, the preparation methods and application prospect of quantum dots are introduced. The electrochemiluminescence principle and luminescence mode of quantum dots are emphatically introduced. The concept and characteristics of nanomaterials are briefly introduced, and the application of nanocrystalline gold is introduced in detail. In chapter 2, water-soluble quantum dots (CdSe) and CdSe/CdS alloy quantum dots (QDs) were prepared. The size and luminescence properties of the QDs were characterized by electron microscopy and spectrometer. The factors affecting the synthesis of quantum dots, the synthesis mechanism and structure composition of quantum dots are discussed. In chapter 3, the electrochemiluminescence (ECL) detection of biogenic amines based on nanomaterials by capillary electrophoresis quantum dots (QDs) was investigated. Including the establishment of electrochemical detection platform, the type of buffer solution in the experimental system, the pH value of buffer solution, the concentration of buffer solution, the separation voltage added at both ends of the capillary, the detection potential set up, the injection time of the capillary, Many factors, such as potential scanning rate, were optimized in detail and the best detection conditions were determined. Six biogenic amines were separated and detected under the best detection conditions. Different biogenic amine molecules enhanced the electrochemiluminescence signal of quantum dots in different degrees. In chapter 4, the amplification effect of gold nanoparticles was used to separate and detect an amplified immunotoxin CTXIB in fish samples by capillary electrophoresis and electrochemical detection. The synthesis of gold nanoparticles was introduced. The gold nanoparticles were modified with horseradish peroxidase and antibody (Ab), and the nano-gold probes labeled with horseradish peroxidase and antibody were obtained. Nanocrystalline gold can adsorb free HRP and Ab in large quantities, so that the sensitivity of detection can be greatly enhanced. The immunotoxin CTXB in fish samples was detected by electrochemical assay using a non-competitive immunoassay model. Conclusion: the experiments mentioned above are summarized briefly.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1;O658.9
【参考文献】
相关期刊论文 前1条
1 谢天尧,郑一宁,莫金垣,赖蓉;一种简易型毛细管电泳电导检测装置及其应用[J];分析测试学报;2000年03期
，本文编号：2164093
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