红光响应眼镜蛇神经毒素PEG-PLGA纳米囊的制备与表征
发布时间：2018-09-06 11:58
【摘要】：以聚乙二醇-聚乳酸-聚乙醇酸嵌段共聚物(PEG-PLGA)为囊材,添加脱镁叶绿酸作为光敏剂,采用复乳法制备了光响应的眼镜蛇神经毒素纳米囊。以纳米囊的包封率、载药量和粒径为指标,采用单因素法对纳米囊的制备条件进行优化;以差示量热扫描分析其热流变性能,并以累积释药量研究其光控释行为。优化的PEGPLGA、眼镜蛇神经毒素及光敏剂脱镁叶绿酸质量配比为40∶12.5∶1,获得的纳米囊包封率为72.3%±3.6%,载药量为15.1%±1.3%,平均粒径为(862±23)nm,电位为(-46.5±3.8)m V,呈紧密球形,光敏剂分布在囊壳;在650 nm半导体激光照射30 min,体外释放明显加快。该纳米囊在不光照时具有增强药物稳定和缓释作用,而红光可促进药物释放,因而可实现光控靶向。
[Abstract]:The optically responsive cobra neurotoxin nanocapsules were prepared using poly (ethylene glycol) polylactic acid-polyethanolid block copolymer (PEG-PLGA) as capsule material and demagnesium chloric acid as Guang Min. Based on the encapsulation efficiency, drug loading and particle size, the preparation conditions were optimized by single factor method, and the thermal fluidity was analyzed by differential scanning calorimetry (DSC), and the photoluminescence behavior was studied by cumulative drug release. The optimized mass ratio of PEGPLGA, neurotoxin and Guang Min was 40: 12.5: 1, the encapsulation efficiency was 72.3% 卤3.6, the drug loading was 15.1% 卤1.3 and the mean particle size was (862 卤23) nm, potential (-46.5 卤3.8) m V,) was compact spherical, and Guang Min was distributed in the capsule. In vitro release was accelerated at 650 nm semiconductor laser irradiation for 30 min,. The nanoparticles can enhance drug stability and sustained release without illumination, while red light can promote drug release, so it can achieve light-controlled targeting.
【作者单位】： 广东岭南职业技术学院;华南理工大学化学与化工学院;
【基金】：国家自然科学基金(81173646) 广州市科技计划项目(1563000123)~~
【分类号】：TB383.1;TQ460.1
本文编号：2226284
[Abstract]:The optically responsive cobra neurotoxin nanocapsules were prepared using poly (ethylene glycol) polylactic acid-polyethanolid block copolymer (PEG-PLGA) as capsule material and demagnesium chloric acid as Guang Min. Based on the encapsulation efficiency, drug loading and particle size, the preparation conditions were optimized by single factor method, and the thermal fluidity was analyzed by differential scanning calorimetry (DSC), and the photoluminescence behavior was studied by cumulative drug release. The optimized mass ratio of PEGPLGA, neurotoxin and Guang Min was 40: 12.5: 1, the encapsulation efficiency was 72.3% 卤3.6, the drug loading was 15.1% 卤1.3 and the mean particle size was (862 卤23) nm, potential (-46.5 卤3.8) m V,) was compact spherical, and Guang Min was distributed in the capsule. In vitro release was accelerated at 650 nm semiconductor laser irradiation for 30 min,. The nanoparticles can enhance drug stability and sustained release without illumination, while red light can promote drug release, so it can achieve light-controlled targeting.
【作者单位】： 广东岭南职业技术学院;华南理工大学化学与化工学院;
【基金】：国家自然科学基金(81173646) 广州市科技计划项目(1563000123)~~
【分类号】：TB383.1;TQ460.1
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