磁性荧光多功能纳米微球的制备及性能研究

发布时间：2018-09-12 04:50

【摘要】：刺激响应型聚合物微球,因其具有随着外部环境刺激,如：温度、pH值、化学环境、光、电场、磁场、机械应力等的变化,而改变自身的物理化学和胶体性质的能力,为其在生物医学和生物技术等领域的药物治疗提供了广泛的科研前景, 主要包括靶向载药、细胞分离和标记、医学成像、诊断和治疗双重功效等。磁性、荧光和温度敏感的多功能材料,因其将单一功能的材料变成多功能组合的材料,从而具有很大的科学和技术研究的发展前景,在多功能成像领域有巨大的潜在研究价值。随着临床科学的大力发展,单一功能的纳米材料已经不能满足需求,研究表明,多功能化的纳米材料将成为今后研究的重点和发展方向。将磁性、荧光和温敏三种功效结合在一起的纳米微球,可以用于光学、MRI成像、温度敏感的多模式的生物医药领域。本章首先制备了发光可聚合配合物单体,通过无皂乳液聚合制备多功能的微球共聚物,结果表明,合成的多功能磁性纳米微球具有良好的超顺磁性、荧光性能和温敏性,可以作为医学研究中潜在的光学探针和MRI造影剂。(1)本文通过无皂乳液聚合法成功合成了多功能性磁性微球,表征了其粒径、形貌、化学结构和组成、荧光和磁性能,并将其作为光学/MRI探针,研究其在大鼠体内的成像效果和积累分布。结果表明,制备的多功能微球的粒径分布在140nm-220nm之间,在特定的激发光下能发射出较强的绿光,易于人眼的分辨。更为重要的是,稀土配合物Tb(AA)3Phen的发光强度到达最大值后可以持续保持。通过对微球进行体内、体外(MRd)以及荧光造影的成像,证实了其对大鼠肝脏和脾脏组织具有较好的造影效果,可以作为光学/MRI探针应用于医学研究。(2)本实验制备了Fe3O4/Poly(MMA-HEMA-Eu(AA)3Phen)纳米微球,结果表明,磁性荧光纳米球具有良好的超顺磁性和荧光性能。TEM图片可以观察到,四氧化三铁纳米粒子被成功包裹于微球内部,微球有清晰的核-壳结构,粒径分布在140-180 nm。通过对微球进行体内、体外(MRI)的造影成像,证实了其对大鼠肝脏和脾脏组织具有较好的造影效果,可以作为潜在的造影剂使用。核磁共振实验中,R2达到568.82 mmM-1s-1。体内磁性和荧光磁共振成像结果,表明微球可以优先积累在肝脏和脾脏组织允许多模式成像,检测在活体内的肿瘤细胞。
[Abstract]:Stimulation-responsive polymer microspheres have the ability to change their physical, chemical and colloidal properties as a result of changes in external environmental stimuli such as temperature, pH, chemical environment, light, electric field, magnetic field, mechanical stress, etc. It provides a wide range of scientific research prospects for drug therapy in biomedicine and biotechnology, including targeting drug delivery, cell separation and labelling, medical imaging, diagnosis and treatment, and so on. Magnetic, fluorescent and temperature-sensitive multifunctional materials have great potential research value in the field of multifunctional imaging, because they turn the single functional materials into multifunctional composite materials, which have a great prospect of scientific and technological research. With the rapid development of clinical science, the single functional nanomaterials can not meet the demand. The research shows that the multifunctional nanomaterials will become the focus and development direction in the future. The nanospheres, which combine magnetic, fluorescent and thermo-sensitive properties, can be used in the field of optical MRI imaging, multi-mode thermo-sensitive biomedicine. In this chapter, the photoluminescence polymeric complex monomer was prepared, and the multifunctional microspheres were prepared by soap-free emulsion polymerization. The results showed that the synthesized multifunctional magnetic nanospheres had excellent superparamagnetism, fluorescence properties and temperature sensitivity. It can be used as a potential optical probe and MRI contrast agent in medical research. (1) Multifunctional magnetic microspheres were successfully synthesized by soap-free emulsion polymerization, and their particle size, morphology, chemical structure and composition, fluorescence and magnetic properties were characterized. It was used as an optical r-MRI probe to study its imaging effect and accumulation distribution in rats. The results show that the size distribution of the multifunctional microspheres is in the range of 140nm-220nm, and can emit strong green light under specific excitation light, which is easy to be resolved by human eyes. More importantly, the luminescence intensity of the rare earth complex Tb (AA) 3Phen can be maintained when it reaches the maximum value. In vivo, in vitro and in vitro (MRd) imaging and fluorescence imaging of the microspheres have proved that the microspheres have a good effect on the liver and spleen of rats, and can be used as an optical / MRI probe for medical research. (2) Fe3O4/Poly (MMA-HEMA-Eu (AA) 3Phen) nanospheres were prepared in this experiment. The results show that the magnetic fluorescent nanospheres have good superparamagnetism and fluorescence properties. Tem images show that Fe _ 2O _ 3 nanoparticles are successfully encapsulated in the microspheres, with a clear core-shell structure and a particle size distribution of 140-180 nm.. The contrast imaging of (MRI) in vivo and in vitro showed that the microspheres had a good effect on the liver and spleen of rats and could be used as a potential contrast agent. The R2 of nuclear magnetic resonance is 568.82 mmM-1s-1.. The results of in vivo magnetic resonance imaging and fluorescence magnetic resonance imaging showed that microspheres could be preferentially accumulated in liver and spleen tissues to allow multi-mode imaging to detect tumor cells in vivo.
【学位授予单位】：湖北大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1

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